2013 Fiscal Year Final Research Report
Single-molecule structure-function analysis of the pH-dependent gating of the KcsA potassium channel
| Project/Area Number |
23370067
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Hirofumi 福井大学, 医学部, 講師 (50324158)
IWAMOTO Masayuki 福井大学, 医学部, 助教 (40452122)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | イオンチャネル / ゲーティング / 構造変化 / 膜内構造 / 集合・離散 |
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| Research Abstract |
The pH-dependent gating of the KcsA potassium channel was examined by enhancing the spacial and temporal resolutions of the diffracted X-ray tracking method. Unlike previous steady-state measurements, the solution pH was jumped by using the caged compound, and transient behavior of the gating conformational change was recorded with the high-speed image sampling (5000 frame/sec). We found that the clockwise twisting was initiated upon acidic pH change. By using the atomic force microscopy (AFM), we revealed the substantial shortening of the longitudinal length of the KcsA channel in the membrane embedded condition. These results indicate that channel shows a spring-like motion, involving simultaneous twisting and shortening, upon opening of the channel.
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[Journal Article] Biased Brownian Stepping Rotation of F oF1-ATP Synthase Driven by Proton Motive Force2013
Author(s)
Watanabe, R., Tabata, K.V., Iino, R., Ueno, H., Iwamoto, M., Oiki, S. and Noji, H
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Journal Title
Nature Commun
Volume: 4
Pages: 1631
DOI
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[Journal Article] ATR-FTIR Spectroscopy Revealed the Different Vibrational Modes of the Selectivity Filter Interacting with K+ and Na+ in the Open and Collapsed Conformations of the KcsA Potassium Channel2012
Author(s)
Furutani, Y., Shimizu, H., Asai, Y., Fukuda, T., Oiki, S. and Kandori, H
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Journal Title
J. Phys. Chem. Lett
Volume: 3
Pages: 3806-3810
DOI
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[Journal Article] Functional Equilibrium of the KcsA Structure Revealed by NMR2012
Author(s)
Imai, S., Osawa, M., Mita, K., Toyonaga, S., Machiyama, A., Ueda, T., Takeuchi, K., Oiki, S. and Shimada, I
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 39634-39641
DOI
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[Remarks] 老木成稔 : イオンチャネルの動的構造と分子機構解明のための1分子研究. 科研費NEWS2013年度Vol.2, 17, 2013.
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[Remarks] 日本生理学会サイエンストピックスNo. 82岩本真幸(老木成稔) : K+チャネルが細胞膜の脂質の環境を感じ取り活性を制御する機構の解明
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[Remarks] 日本生理学会サイエンストピックスNo. 92角野歩(老木成稔) : カリウムチャネルKcsAのゲート開閉と連動した膜中集合・離散ダイナミクス
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