2013 Fiscal Year Final Research Report
Exploration of physiological functions of mammalian septins with genetically engineered mouse lines
| Project/Area Number |
23370084
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Nagoya University |
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Principal Investigator |
KINOSHITA Makoto 名古屋大学, 理学(系)研究科(研究院), 教授 (30273460)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | セプチン / アクチン / 微小管 / 細胞骨格 / 細胞表層 / アセチル化 / 神経突起 / シナプス伝達 |
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| Research Abstract |
Septins are evolutionarily conserved polymerizing GTPases like the major cytoskeletal nucleotide-binding proteins of tubulins and actins, however, their physiological functions in metazoans are largely unknown. To address this: 1) We have established a line of conditional SEPT7 knockout mice and uncovered that septins promote dendrite and axon development by negatively regulating microtubule stability via HDAC6-mediated deacetylation. 2) We established prion-promoter-driven SEPT4 transgenic mice and uncovered that chronic overload of SEPT4, a parkin substrate that aggregates in Parkinson's disease, causes behavioral alterations but not neurodegeneration in mice. These and other findings, in addition to mouse lines and a set of reagents created in this study, will help understand cellular/molecular mechanisms of septin functions in relation to other cytoskeletal systems in physiologically relevant contexts.
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Research Products
(28 results)
