2013 Fiscal Year Final Research Report
Multi-functionality of dynamin family and mechanism of integrated control
| Project/Area Number |
23370089
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Okayama University |
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Principal Investigator |
TAKEI Kohji 岡山大学, 医歯(薬)学総合研究科, 教授 (40322226)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (80325092)
TANEBE Kenji 岡山大学, 大学院・医歯薬学総合研究科, 助教 (80423341)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | ダイナミン / コルタクチン / アクチン / 成長円錐 / フィロポディア / 阻害剤 |
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| Research Abstract |
We have clarified a novel regulatory mechanism of actin dynamics by dynamin/cortactin complex. The complex is ring-shaped and it changed the conformation upon dynamin GTP hydrolysis, from open ring to close ring. By the open-cloze motion, the complex bundled F-actins and stabilized the actin bundles. Furthermore, we identified N'-[4-(dipropylamino)benzylidene]-2-hydroxybenzohydrazide(DBHA)as a dynamin inhibitor that suppress dynamin-dependent actin regulation. DBHA inhibited recruitment of dynamin to the leading edge of migrating cancer cell line and ruffle formation. It also showed inhibitory effect in cell migration, invasion, and proliferation.
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[Journal Article] N'-[4-(dipropylamino)benzylidene]-2-hydroxybenzohydrazide is a dynamin GTPase inhibitor that suppresses cancer cell migration and invasion by inhibiting actin polymerization2013
Author(s)
Yamada, H., Abe, T., Li, S.A., Tago, S., Huang, P., Watanabe, M., Ikeda, S., Ogo, N., Asai, A., Takei, K
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Journal Title
Biochem Biophys Res Commun
Volume: 443
Pages: 511-517
DOI
Peer Reviewed
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[Journal Article] Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia2013
Author(s)
Yamada, H., Abe, T., Satoh, A., Okazaki, N., Tago, S., Kobayashi, K., Yoshida, Y., Oda, Y., Watanabe, M., Tomizawa, K., Matsui, H., and Takei, K
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Journal Title
J Neurosci
Volume: 33
Pages: 4514-4526
DOI
Peer Reviewed
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[Journal Article] REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer2012
Author(s)
Hirata, T., Watanabe, M., Kaku, H., Kobayashi, Y., Yamada, H., Sakaguchi, M., Takei, K., Huh, N.H., Nasu, Y, Kumon, H
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Journal Title
Int J Oncol
Volume: 41
Pages: 559-564
DOI
Peer Reviewed
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[Journal Article] A novel gene expression system for detecting viable bladder cancer cells2012
Author(s)
Ueki, H., Watanabe, M., Kaku, H., Huang, P., Li, S.A., Ochiai, K., Hirata, T., Noguchi, H., Yamada, H., Takei, K., Nasu, Y, Kashiwakura Y, and Kumon H
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Journal Title
Int J Oncol
Volume: 41
Pages: 135-140
Peer Reviewed
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[Journal Article] The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice2012
Author(s)
Suzuki, M., Tanaka, H., Tanimura, A., Tanabe, K., Oe, N., Rai, S., Kon, S., Fukumoto, M., Takei, K., Abe, T., Matsumura, I., Kanakura, Y., and Watanabe, T
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Receptor sorting within endosomal trafficking pathway is facilitated by dynamic actin filaments2011
Author(s)
Ohashi, E., Tanabe, K., Henmi, Y., Mesaki, K., Kobayashi, Y., and Takei, K
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Journal Title
DOI
Peer Reviewed
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[Patent(Industrial Property Rights)] フルボキサミンを配合することを特徴とする分子標的型抗癌剤(特に抗脳腫瘍治療薬剤:第二医薬品用途)2012
Inventor(s)
道上宏之, 林圭一郎, 松井秀樹, 山田浩司, 竹居孝二, 宮地弘幸, 浅井章良
Industrial Property Rights Holder
道上宏之, 林圭一郎, 松井秀樹, 山田浩司, 竹居孝二, 宮地弘幸, 浅井章良
Industrial Property Rights Type
特許
Industrial Property Number
特願2012-263317
Filing Date
2012-11-30
