2013 Fiscal Year Final Research Report
Analysis of regulatory mechanisms of epigenetic gene regulation by a complex of nuclear matrix protein and noncoding RNA
| Project/Area Number |
23370093
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
NAKAGAWA Shinichi 独立行政法人理化学研究所, 中川RNA生物学研究室, 准主任研究員 (50324679)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 核マトリクス / hnRNP U / インプリンティング / アリル特異的発現 |
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| Research Abstract |
In the human and mouse, vast majority of the genome is transcribed into RNAs, producing a huge number of non protein-coding RNAs. These noncoding RNAs associate with chromatin-modifying complexes, and are assumed to regulate epigenetic gene expression. In this study, we have performed functional analysis of a nuclear matrix protein hnRNP U that regulate chromosomal localization of Xist, a renowned noncoding RNA involved in the X chromosome inactivation. We found that hnRNP U also regulates the chromosomal localization and function of noncoding RNAs such as Kcnq1ot1 and Airn, and the allele-specific expression of the imprinted loci were impaired upon depletion of hnRNP U. We also performed genome-wide gene expression analysis of the hnRNP U depleted cells, and found that multiple allele-specific expression were also disturbed. We found a novel noncoding RNA expressed from the allele-specific locus, and its transcripts were also associated with the chromosome in a mono-allelic manner.
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[Remarks] 中川真一,石田賢太郎「mRNA 非翻訳領域のレトロトランスポゾン挿入配列による転写後遺伝子発現制御- 潜在的な遺伝的変異の蓄積と表現型としての多様性の急激な解放」 実験医学増刊号, 31(7) 115-121 (2013)
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[Remarks] 中川真一「転移因子・ウイルスと宿主のせめぎ合いによりもたらされるゲノム進化」実験医学増刊号, 31 (7), 83-90 (2013)
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[Remarks] 中川真一 核内構造体に活躍の場を求めたノンコーディング RNA たち 細胞工学, 734-739 (2011)
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[Remarks] 中川真一, 影山裕二 長鎖 ncRNA 研究のはじまりの終わり実験医学, 29 (11),1702-1707 (2011)
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