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2013 Fiscal Year Final Research Report

Development of genomics assisted breeding system of salt tolerant barley

Research Project

  • PDF
Project/Area Number 23380007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionOkayama University

Principal Investigator

SATO Kazuhiro  岡山大学, その他部局等, 教授 (60215770)

Co-Investigator(Kenkyū-buntansha) HISANO Hiroshi  岡山大学, 資源生物科学研究所, 助教 (70415454)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywords耐塩性 / 不良環境耐性 / オオムギ / DNAマーカー / 遺伝子単離 / 形質転換 / cDNA
Research Abstract

cDNA sequences are generated by an efficient analysis system and aligned with full length cDNA sequences of Haruna Nijo to develop genome wide SNP detection system. The array system is used to genotype recombinant inbred lines and recombinant chromosome substitution lines for genetic map development and estimation of substitution segments. Salt tolerance of these lines are evaluated to map tolerance QTLs and their responsible substitution segments. On the other hand, a previously isolated tolerance gene by subtraction method is used to make transgenic plant to evaluate its salt tolerance. Markers are also developed to narrow down tolerance genes. The recombinant chromosome substitution liens carrying tolerance gene segments are developed to be used as isogenic lines for salt tolerance.

  • Research Products

    (15 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (5 results) Book (2 results)

  • [Journal Article] Mapping non-recombining regions in barley using multicolor FISH2013

    • Author(s)
      Karafiá tová M., Bartoš J., Kopecký D., Ma L., Sato K., Houben A., Stein N. and Dolež el J.
    • Journal Title

      Chromosome Res

      Volume: 21 Pages: 739-751

    • DOI

      10.1007/s10577-013-9380-x

    • Peer Reviewed
  • [Journal Article] オオムギゲノム多様性の解析と育種への応用2013

    • Author(s)
      佐藤和広
    • Journal Title

      育種学研究

      Volume: 15 Pages: 167-172

  • [Journal Article] Discovery of high-confidence SNPs from large-scale de novo analysis of leaf transcripts of Aegilops tauschii, a wild wheat progenitor2012

    • Author(s)
      Iehisa, M., Shimizu, A., Sato, K., Nasuda, S., and Takumi, S
    • Journal Title

      DNA Res

      Volume: 19 Pages: 487-197

    • DOI

      10.1093/dnares/dss028

    • Peer Reviewed
  • [Journal Article] A physical, genetic and functional sequence assembly of the barley genome2012

    • Author(s)
      The International Barley Genome Sequencing Consortium (IBSC)
    • Journal Title

      Nature

      Volume: 491 Pages: 711-716

    • DOI

      10.1038/nature11543

    • Peer Reviewed
  • [Journal Article] Development of genetic map and alignment of recombinant chromosome substitution lines from a cross of EST donors by high accuracy SNP typing in barley2011

    • Author(s)
      Sato, K., Close, T., Bhat, P., Muñ oz-Amatriaí n, M., and Muehlbauer, G
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Pages: 728-737

    • Peer Reviewed
  • [Journal Article] Construction and sequence analysis of barley 24, 783 full-length cDNAs2011

    • Author(s)
      Matsumoto, T, Tanaka, T., Sakai, H., Amano, N., Kanamori, H., Kurita, K., Kikuta, A., Kamiya, K., Yamamoto, M., Ikawa, H., Fujii, N., Hori, K., Itoh, T., Takeda, K., and Sato, K
    • Journal Title

      Plant Physiol

      Volume: 156 Pages: 20-28

    • DOI

      10.1104/pp.110.171579

    • Peer Reviewed
  • [Journal Article] Unlocking an archetypal 5.1 Gb Triticeae genome by barley chromosomal genomics2011

    • Author(s)
      Mayer, K., et al.
    • Journal Title

      Plant Cell

      Volume: 23 Pages: 1249-1263

    • DOI

      10.1105/tpc.110.082537

    • Peer Reviewed
  • [Journal Article] An improved consensus linkage map of barley based on flow-sorted chromosomes and SNP markers2011

    • Author(s)
      Muñ oz-Amatriaín, M., et al.
    • Journal Title

      The Plant Genome

      Volume: Vol.4No.3

    • DOI

      10.3835/plantgenome2011.08.0023

    • Peer Reviewed
  • [Presentation] オオムギ遺伝資源コレクションの全ゲノム SNP フィンガープリンティング2013

    • Author(s)
      佐藤和広・元井由加
    • Organizer
      日本育種学会講演会
    • Place of Presentation
      鹿児島
    • Year and Date
      20131012-13
  • [Presentation] Barley natural variation and adaptation to global environments2013

    • Author(s)
      Kazuhiro Sato
    • Organizer
      16th Australian Barley Technical Symposium, Sofitel Melbourne on Collins
    • Place of Presentation
      Melbourne, Australia
    • Year and Date
      20130908-11
  • [Presentation] オオムギゲノム多様性の解析と育種への応用 (平成24年度日本育種学会賞受賞講演)2013

    • Author(s)
      佐藤和広
    • Organizer
      日本育種学会講演会
    • Place of Presentation
      東京
    • Year and Date
      20130327-28
  • [Presentation] オオムギゲノム配列の概要2012

    • Author(s)
      佐藤和広
    • Organizer
      ムギ類研究会
    • Place of Presentation
      つくば
    • Year and Date
      20121128-29
  • [Presentation] cDNA 解析によるオオムギゲノム育種用 DNA マーカーの開発2012

    • Author(s)
      佐藤和広・元井由加
    • Organizer
      日本育種学会講演会
    • Place of Presentation
      京都
    • Year and Date
      20120914-15
  • [Book] 植物の遺伝と育種第2版2013

    • Author(s)
      福井希一・向井康比古・佐藤和広
    • Total Pages
      238
    • Publisher
      朝倉書店
  • [Book] Genetic diversity and germplasm management -wild barley, landraces, breeding materials. Kumlehn, J., and Stein, N., (eds.) Biotechnological Approaches to Barley Improvement. Biotechnology in Agriculture and Forestry

    • Author(s)
      Sato, K., Flavell, A., Russell, J., Bö rner, A., and Valkoun, J
    • Publisher
      Springer(印刷中)

URL: 

Published: 2015-06-25  

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