Identification of putative plant defense activators of novel mode of action.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Tokyo University of Science
• Principal Investigator: KUCHITSU Kazuyuki 東京理科大学, 理工学部, 教授 (50211884)
• Co-Investigator(Renkei-kenkyūsha): KURUSU Takamitsu 東京工科大学, 応用生物学部, 助教 (50422499) ; KAYA Hidetaka 農業生物資源研究所, 研究員 (80398825)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 膜交通系 / 活性酸素種 / カルシウムイオン / 植物免疫 / 植物防除剤 / 植物免疫活性化剤 / 抵抗性誘導剤 / ケミカルバイオロジー

Research Abstract

Plant defense activators are chemicals that induce disease resistance by enhancing plant immunity upon pathogen infection. Defense signaling pathways induced by cryptogein, a proteinaceous elicitor derived from a pathogenic oomycete, in tobacco BY-2 cells has been extensively studied as a simple model system. Based on the correlation between the temporal pattern of enzymatic production of reactive oxygen species (ROS) and downstream defense responses, we established a new high-throughput screening method to identify novel lead compounds for plant defense activators. From a total of 11,000 compounds screened, we selected 58 candidates for activators. We further examined the effects of selected compounds on the expression of defense-related genes in Arabidopsis plants, and discovered some chemicals that potentially enhance jasmonic acid-dependent signaling pathway as putative plant defense activators with a novel mode of action.

Research Products

• [Journal Article] Seasonal changes in ice nucleation activity in blueberry stems and effects of cold treatments in vitro (2014)
  • Author(s): Kishimoto T, Sekozawa Y, Y amazaki Y, Murakawa H, Kuchitsu K, Ishikawa M
  • Journal Title: Environmental and Experimental Botany Volume: (印刷中)
  • DOI: 10.1016/j.envexpbot.2014.02.010
  • Peer Reviewed
• [Journal Article] Discovering Compounds That Activate Plant Immunity Using Machine Learning (2014)
  • Author(s): Yoshida T, Kitahata N, Ohwada H, Kuchitsu K
  • Journal Title: Proceedings of the 6th International Conference on Bioinformatics and Computational Biology Volume: (in press)
  • Peer Reviewed
• [Journal Article] OsATG7 is required for autophagy-dependent lipid metabolism in rice post-meiotic anther development (2014)
  • Author(s): Kurusu T, Koyano T, Hanamata S, Kubo T, Noguchi Y, Y agi C, Nagata N, Y amamoto T, Ohnishi T, Okazaki Y, Kitahata N, Ando D, Ishikawa M, Wada S, Miyao A, Hirochika H, Shimada S, Makino A, Saito K, Ishida H, Kinoshita T, Kurata N, Kuchitsu K
  • Journal Title: Autophagy Volume: 10 Pages: 860-870
  • URL: https://www.landesbioscience.com/journals/autophagy/article/28279/
  • Peer Reviewed
• [Journal Article] Ca^<2+> -activated ROS production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth (2013)
  • Author(s): Kaya H, Nakajima R, Iwano M, Kanaoka MM, Kimura S, T akeda S, Kawarazaki T, Senzaki E, Hamamura Y, Higashiyama T, T akayama S, Abe M, Kuchitsu K
  • Journal Title: Plant Cell Volume: 26(3) Pages: 1069-1080
  • DOI: 10.1105/tpc.113.120642
  • Peer Reviewed
• [Journal Article] Abscisic acid induced freezing tolerance in chilling-sensitive suspension cultures and seedlings of rice (2013)
  • Author(s): Shinkawa R, Morishita A, Amikura K, Machida R, Murakawa H, Kuchitsu K, Ishikawa M
  • Journal Title: BMC Research Notes Volume: 6 Pages: 351
  • DOI: 10.1186/1756-0500-6-351
  • Peer Reviewed
• [Journal Article] A low temperature-inducible protein AtSRC2 enhances the ROS-producing activity of NADPH oxidase AtRbohF (2013)
  • Author(s): Kawarazaki T, Kimura S, Iizuka A, Hanamata S, Nibori H, Michikawa M, Imai A, Abe M, Kaya H, Kuchitsu K
  • Journal Title: Biochim et Biophys Acta- Mol. Cell Res Volume: 1833 Pages: 2775-2780
  • DOI: 10.1016/j.bbamcr.2013.06.024
  • Peer Reviewed
• [Journal Article] An S-type anion channel SLAC1 is involved in cryptogein-induced ion fluxes and modulates hypersensitive responses in tobacco BY -2 cells (2013)
  • Author(s): Kurusu T, Saito K, Horikoshi S, Hanamata S, Negi J, Yagi C, Kitahata N, Iba K, Kuchitsu K
  • Journal Title: PLOS ONE Volume: 8 Pages: e70623
  • DOI: 10.1371/journal.pone.0070623
  • Peer Reviewed
• [Journal Article] Hedrich R, On the cellular site of two-pore channel TPC1 action 1 in the Poaceae (2013)
  • Author(s): Dadacz-Narloch B, Kimura S, Kurusu T, Farmer EE, Becker D, Kuchitsu K
  • Journal Title: New Phytol Volume: 200 Pages: 663-674
  • DOI: 10.1111/nph.12402
  • Peer Reviewed
• [Journal Article] Tobacco MAP kinase phosphatase (NtMKP1) negatively regulates wound response and induced resistance against necrotrophic pathogens and lepidopteran herbivores (2013)
  • Author(s): Oka K, Amano Y, Katou S, Seo S, Kawazu K, Mochizuki A, Kuchitsu K, Mitsuhara I
  • Journal Title: Mol Plant-Microbe Interact Volume: 26 Pages: 668-675
  • DOI: 10.1094/MPMI-11-12-0272-R
  • Peer Reviewed
• [Journal Article] The calcineurin B-like calcium sensors CBL1 and CBL9 together with their interacting protein kinase CIPK26 regulate the Arabidopsis NADPH oxidase RBOHF (2013)
  • Author(s): Drerup M, Schlücking K, Hashimoto K, Manishankar P, Steinhorst L, Kuchitsu K, Kudla J
  • Journal Title: Molecular Plant Volume: 6 Pages: 559-569
  • DOI: 10.1093/mp/sst009
  • Peer Reviewed
• [Journal Article] Coordination structures of Mg^<2+> and Ca^<2+> in three types of tobacco calmodulins in solution : Fourier-transform infrared spectroscopic studies of side-chain COO groups (2013)
  • Author(s): Suzuki N, Imai LF, Kato K, Nagata K, Ohashi Y, Kuchitsu K, T anokura M, Sakamoto A, Nara M, Nakano M,Yonezawa N
  • Journal Title: Biopolymers Volume: 99 Pages: 472-483
  • DOI: 10.1002/bip.22203
  • Peer Reviewed
• [Journal Article] Plant mechanosensing and Ca^<2+> transport (2013)
  • Author(s): Kurusu T, Kuchitsu K, Nakano M, Nakayama Y, Iida H
  • Journal Title: Trends in Plant Sci Volume: 18 Pages: 227-233
  • DOI: 10.1016/j.tplants.2012.12.002
  • Peer Reviewed
• [Journal Article] The CBL-interacting protein kinase CIPK26 is a novel interactor of Arabidopsis NADPH oxidase AtRbohF that negatively modulates its ROS-producing activity in a heterologous expression system (2013)
  • Author(s): Kimura S, Kawarazaki T, Nibori H, Michikawa M, Imai A, Kaya H, Kuchitsu K
  • Journal Title: J. Biochem Volume: 153(2) Pages: 191-195
  • DOI: 10.1093/jb/mvs132
  • Peer Reviewed
• [Journal Article] Invivoimaging and quantitative monitoring of autophagic flux in tobacco BY-2 cells (2013)
  • Author(s): Hanamata S, Kurusu T, Okada M, Suda A, Kawamura K, T sukada E, Kuchitsu K
  • Journal Title: Plant Signal. Behav Volume: 8 Pages: e22510
  • DOI: 10.4161/psb.22510
  • Peer Reviewed
• [Journal Article] Intracellular localization and physiological function of a rice Ca_<2+> -permeable channel O_sTPC1 (2012)
  • Author(s): Kurusu T, Hamada H, Koyano T, Kuchitsu K
  • Journal Title: Plant Signal. Behav Volume: 7 Pages: 1428-1430
  • DOI: 10.4161/psb.22086
  • Peer Reviewed
• [Journal Article] Reactive oxygen species production and activation mechanism of the rice NADPH oxidase OsRbohB (2012)
  • Author(s): Takahashi S, Kimura S, Kaya H, Iizuka A, Wong HL, Shimamoto K, Kuchitsu K
  • Journal Title: J. Biochem Volume: 152 Pages: 37-43
  • DOI: 10.1093/jb/mvs044
  • Peer Reviewed
• [Journal Article] Tandemly arranged chalcone synthase A genes contribute to the spatially regulated expression of siRNA and the natural bicolor floral phenotype in Petunia hybrida (2012)
  • Author(s): Morita Y, Saito R, Ban Y, T anikawa N, Kuchitsu K, Ando T, Y oshikawa M, Habu Y, Ozeki Y, Nakayama M
  • Journal Title: Plant J Volume: 70 Pages: 739-749
  • DOI: 10.1111/j.1365-313X.2012.04908.x
  • Peer Reviewed
• [Journal Article] Roles of a putative mechanosensitive plasma membrane Ca 2+ -permeable channel OsMCA1 in generation of reactive oxygen species and hypo-osmotic signaling in rice (2012)
  • Author(s): Kurusu T, Iida H, Kuchitsu K
  • Journal Title: Plant Signal. Behav Volume: 7 Pages: 796-798
  • DOI: 10.4161/psb.20521
  • Peer Reviewed
• [Journal Article] Regulation of a proteinaceous elicitor-induced Ca^<2+> influx and production of phytoalexins by a putative voltage-gated cation channel (2012)
  • Author(s): Hamada H, Kurusu T, Okuma E, Nokajima H, Kiyoduka M, Koyano T, Sugiyama Y, Okada K, Koga J, Saji H, Miyao A, Hirochika H, Y amane H, Murata Y, Kuchitsu K
  • Journal Title: OsTPC1, in cultured rice cells., J Biol. Chem Volume: 287 Pages: 9931-9939
  • DOI: 10.1074/jbc.M111.337659
  • Peer Reviewed
• [Journal Article] Plasma membrane protein OsMCA1 is involved in regulation of hypo-osmotic shock-induced Ca^<2+> influx and modulates generation of reactive oxygen species in cultured rice cells (2012)
  • Author(s): Kurusu T, Nishikawa D, Y amazaki Y, Gotoh M, Nakano M, Hamada H, Yamanaka T, Iida K, Nakagawa Y, Saji H, Shinozaki K, Iida H, Kuchitsu K
  • Journal Title: BMC Plant Biol Volume: 12 Pages: e11
  • DOI: 10.1186/1471-2229-12-11
  • Peer Reviewed
• [Journal Article] Involvement of the putative Ca^<2+> -permeable mechanosensitive channels, NtMCA1 and NtMCA2, in Ca^<2+> uptake, Ca^<2+> -dependent cell proliferation and mechanical stress-induced gene expression in tobacco (Nicotia na ta ba cum) BY -2 cells (2012)
  • Author(s): Kurusu T, Yamanaka T, Nakano M, Takiguchi A, Ogasawara Y, Hayashi T, Iida K, Hanamata S, Shinozaki K, Iida H, Kuchitsu K
  • Journal Title: J Plant Res Volume: 125 Pages: 555-568
  • DOI: 10.1007/s10265-011-0462-6
  • Peer Reviewed
• [Journal Article] Fungal elicitor-induced retardation and its restoration of root growth in tobacco seedlings (2012)
  • Author(s): Kawaguchi Y, Nishiuchi T, Kodama H, Nakano T, Nishimura K, Shimamura K, Y amaguchi K, Kuchitsu K, Shinshi H, Suzuki K
  • Journal Title: Plant Growth Regulation Volume: 66 Pages: 59-68
  • DOI: 10.1007/s10725-011-9629-3
  • Peer Reviewed
• [Journal Article] Protein phosphorylation is a prerequisite for the Ca^<2+> -dependent activation of Arabidopsis NADPH oxidases and may function as a trigger for the positive feedback regulation of Ca^<2+> and reactive oxygen species., Biochim. Biophys (2012)
  • Author(s): Kimura S, Kaya H, Kawarazaki T, Hiraoka G, Senzaki E, Michikawa M, Kuchitsu K
  • Journal Title: Acta-Mol. Cell Res Volume: 1823 Pages: 398-405
  • DOI: 10.1016/j.bbamcr.2011.09.011
  • Peer Reviewed
• [Journal Article] A novel calcium binding site in the slow vacuolar cation channel TPC1 senses luminal calcium level (2011)
  • Author(s): Dadacz-Narloch B, Beyhl D, Larisch C, López-Sanjurjo EJ, Reski R, Kuchitsu K, Müller T, Becker D, Schoenknecht G and Hedrich R
  • Journal Title: Plant Cell Volume: 23 Pages: 2696-2707
  • DOI: 10.1105/tpc.111.086751
  • Peer Reviewed
• [Journal Article] Opening a New Era of ABA Research (2011)
  • Author(s): Nambara E, Kuchitsu K
  • Journal Title: J. Plant Res Volume: 124(4) Pages: 431-435
  • DOI: 10.1007/s10265-011-0437-7
  • Peer Reviewed
• [Journal Article] Cryptogeininduced cell cycle arrest at G2 phase is associated with inhibition of cyclindependent kinases, suppression of expression of cell cycle-related genes and protein degradation in synchronized tobacco BY -2 cells (2011)
  • Author(s): Ohno, R., Kadota, Y ., Fujii, S., Sekine, M., Umeda, M., Kuchitsu, K
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 922-932
  • DOI: 10.1093/pcp/pcr042
  • Peer Reviewed
• [Journal Article] Dynamic reorganization and function of the cytoskeleton and vacuoles in defense responses and programmed cell death (2011)
  • Author(s): Higaki, T ., Kurusu, T ., Hasezawa, S., Kuchitsu, K
  • Journal Title: J. Plant Res Volume: 124 Pages: 315-324
  • DOI: 10.1007/s10265-011-0408-z
  • Peer Reviewed
• [Journal Article] Negative Feedback Regulation of Microbe-Associated Molecular Pattern-Induced Cytosolic Ca^<2+> Transients by Protein Phosphorylation (2011)
  • Author(s): Kurusu, T ., Hamada, H., Sugiyama, Y ., Y agala, T ., Kadota, Y ., Furuichi, T ., Hayashi, T ., Umemura, K., Komatsu, S., Miyao, A., Hirochika, H., Kuchitsu, K
  • Journal Title: J. Plant Res Volume: 124 Pages: 415-424
  • DOI: 10.1007/s10265-010-0388-4
  • Peer Reviewed
• [Presentation] オートファジー動態の定量的モニタリング (2014)
  • Author(s): 花俣繁, 来須孝光, 岡田全朗, 須田晶子, 河村康希, 塚田慧美, 朽津和幸
  • Organizer: 第55回日本植物生理学会年会シンポジウム「顕微鏡で測る」
  • Place of Presentation: 富山大学
  • Year and Date: 20140318-20
• [Presentation] Possible involvement of reactive carbonyls species in ROS-mediated regulation of plant immunity and programed cell death. 活性酸素シグナルによる植物免疫・細胞死の制御と活性カルボニル (2014)
  • Author(s): 朽津和幸, 北畑信隆, 助川夏雄, 中内愛由子, 賀 屋 秀 隆 , 木 村 幸 恵 , 真 野 純 一
  • Organizer: 第55回日本植物生理学会年会シンポジウム「カルボニルシグナルの作用と制御
  • Place of Presentation: 富山大学
  • Year and Date: 20140318-20
• [Presentation] 活性酸素-Ca^<2+>シグナルネットワークによる植物の生体防御・発生の制御～植物の免疫力強化を目指して～ (2014)
  • Author(s): 朽津和幸
  • Organizer: 第7回レドックス ・ ライフイノベーションシンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2014-03-07
• [Presentation] 植物のシグナル伝達系におけるイオン・活性酸素種の役割 (2013)
  • Author(s): 朽津和幸, 来須孝光, 北畑信隆, 木村幸恵, 河原崎朋子, 賀屋秀隆
  • Organizer: 日本植物生理学会シンポジウム
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 20130321-23
• [Presentation] 植物と活性酸素の深い関係 : 悪魔か天使か? (2013)
  • Author(s): 朽津和幸
  • Organizer: イメージングフロンティアシンポジウム 2013
  • Place of Presentation: 東京理科大学野田キャンパス
  • Year and Date: 2013-12-21
• [Presentation] ROS-Ca^<2+> signaling network regulating innate immunity , programmed cell death and development in plants (2013)
  • Author(s): 朽津和幸
  • Organizer: Indo-Japan Joint Workshop on "Signal sensing and transduction in photosynthetic organisms-from cyanobacteria to land plants"
  • Place of Presentation: University of Hyderabad, India
  • Year and Date: 2013-12-16
• [Presentation] 植物のシグナル伝達系における活性酸素, イオンチャネルの役割 (2013)
  • Author(s): 朽津和幸, 北畑信隆, 来須孝光
  • Organizer: 日本生化学会シンポジウム「多様なアプローチから解明が進む植物シグナル伝達研究」
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-09-12
• [Presentation] 植物の生体防御における活性酸素-Ca^<2+> (2013)
  • Author(s): 朽津和幸
  • Organizer: シグナルネットワークとその制御日本生体防御学会
  • Place of Presentation: くまもと森都心プラザ プラザホール
  • Year and Date: 2013-07-11
• [Presentation] 植物の生き方と情報ネットワーク (2012)
  • Author(s): 朽津和幸
  • Organizer: 京都大学数理解析研究所セミナー
  • Place of Presentation: 京都大学
  • Year and Date: 2012-08-01
• [Presentation] Chemical regulation of defense signaling network and immune responses in plants (2012)
  • Author(s): 朽津和幸, 来須孝光
  • Organizer: 日本植物生理学会シンポジウム
  • Year and Date: 2012-03-17
• [Presentation] 植物の生老病死の化学制御 (2011)
  • Author(s): 朽津和幸
  • Organizer: 第84回日本生化学会大会, シンポジウム「植物の生老病死の化学制御」 (オーガナイザー朽津和幸,刑部祐里子)
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20110921-24
• [Presentation] シグナルネットワークの制御による植物免疫の活性化 (2011)
  • Author(s): 朽津和幸, 来須孝光
  • Organizer: 第84回日本生化学会大会,シンポジウム「植物の生老病死の化学制御」
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20110921-24
• [Presentation] シロイヌナズナの活性酸素種生成酵素 AtRbohA-Jの活性制御機構と機能分担 (2011)
  • Author(s): 河原崎朋子, 飯塚文子, 今井亜耶, 村上祐樹, 森恭一郎, 賀屋秀隆, 朽津和幸
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20110921-24
• [Presentation] 植物免疫の制御とオートファジー～培養細胞からのアプローチ～ (2011)
  • Author(s): 朽津和幸, 花俣繁, 来須孝光
  • Organizer: 日本植物細胞分子生物学会シンポジウム, 「植物のオートファジーと老化プロセス」
  • Place of Presentation: 九州大学
  • Year and Date: 20110906-08
• [Presentation] Regulation of ROS production in early signaling network in innate immunity in cultured plant cells (2011)
  • Author(s): Kazuyuki Kuchitsu
  • Organizer: 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 20110705-08
• [Presentation] 植物の生体防御と活性酸素-Ca^<2+>シグナルネットワーク (2011)
  • Author(s): 朽津和幸
  • Organizer: 生体触媒化学シンポジウム
  • Place of Presentation: 慶應義塾大学薬学部(芝共立キャンパス)
  • Year and Date: 2011-12-22
• [Presentation] 植物の Ca^<2+> -活性酸素シグナルネットワーク (2011)
  • Author(s): 朽津和幸, 濱田晴康, 来須孝光, 賀屋秀隆
  • Organizer: 日本植物学会第75回大会シンポジウム「植物の Ca^<2+>シグナリング～園芸学への展開～」
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2011-09-17
• [Presentation] シグナルネットワークの制御による植物免疫の活性化 (2011)
  • Author(s): 朽津和幸
  • Organizer: 日本農芸化学会藪田セミナー, 東京大学大学院農学生命科学研究科中島董一郎記念ホール
  • Year and Date: 2011-07-14
• [Book] Regulation of V acuole-Mediated Programmed Cell Death During Innate Immunity and Reproductive Development in Plants., "Sexual Reproduction in Animals and Plants", Sawada H, Inoue N, Iwano M (Eds.) (2013)
  • Author(s): Koyano T , Kurusu T, Hanamata S, Kuchitsu K
  • Total Pages: 431-440
  • Publisher: Springer
• [Book] Plant signaling networks involving reactive oxygen species and Ca^<2+> ., Handbook on Reactive Oxygen Species (ROS): Formation Mechanisms, Physiological Roles and Common Harmful Effects., Suzuki M, Yamamoto S (Eds) (2013)
  • Author(s): Kurusu T, Kimura S, T ada Y , Kaya H, Kuchitsu K
  • Total Pages: 315-324
  • Publisher: Nova Science Publishers, Inc. NY , USA
• [Book] Signaling Networks Involving Reactive Oxygen Species and Ca^<2+> in Plants., QUANTUM BIO-INFORMA TICS Vedited by Accardi, L., Freudenberg, W., Ohya, M (2013)
  • Author(s): Kuchitsu K
  • Total Pages: 403-407
  • Publisher: World Scientific
• [Remarks] 2013.11.8 科学新聞 植物の免疫を制御.陰イオンチャネルタンパク質(新聞報道)
• [Remarks] 2013.9.10 日経バイオテク, 日経バイオテクオンライン 東京理科大が陰イオンチャネルによる植物免疫制御を発見, 化合物の特許を 3 社が評価中(新聞報道)
• [Remarks] 2012.3.9 科学新聞 東京理科大, 植物の免疫の仕組み解明へ 「イネの抗菌化合物」 合成制御タンパク質発見 東京理科大学 朽津和幸教授(新聞報道)
• [Remarks] 2012.2.29 日経産業新聞 イネの抗菌化合物合成の制御 東京理科大学 朽津和幸教授(新聞報道)
• [Remarks] 2012.2.23 化学工業日報 イネの抗菌化合物合成制御たん白耐病性機構の一端 解明 東京理科大学 朽津和幸教授(新聞報道)
• [Remarks] 2013.11.23-24 東京理科大学サイエンス夢工房「自分の DNA を見てみよう!～バイオイメージングの世界～」 「生きた光るクラゲや, 青いカーネーションを見ながら, 遺伝子・バイオテクノロジーについて考えてみよう!」(アウトリーチ活動)
• [Remarks] 2013.11.23 東京理科大学生涯学習センター公開講座「自分のDNAを観察し, いのちを考える～光るクラゲや青いカーネーションからバイオテクノロジーまで～」(アウトリーチ活動)
• [Remarks] 2013.8.8 東京理科大学オープンキャンパス実験講座「自分のDNAを見てみよう!～バイオイメージングの世界～」(アウトリーチ活動)
• [Remarks] 2013.8.7 東京理科大学生涯学習センター公開講座「自分のDNAを観察し, いのちを考える～光るクラゲや青いカーネーションからバイオテクノロジーまで～」(アウトリーチ活動)
• [Remarks] 2013.6.22 専修大学松戸高等学校出張授業(アウトリーチ活動)
• [Remarks] 2013.6.20 千葉県立鎌ヶ谷葛飾高等学校出張授業(アウトリーチ活動)
• [Remarks] 2013.4.19 私立茨城高等学校出張授業(アウトリーチ活動)
• [Remarks] 2012.11.24 東京理科大学サイエンス夢工房「自分のDNAを見てみよう!～バイオイメージングの世界～」 「生きた光るクラゲや, 青いカーネーションを見ながら, 遺伝子・バイオテクノロジーについて考えてみよう!」(アウトリーチ活動)
• [Remarks] 2012.10.24 朝日カルチャーセンター講座「四季の花めぐり 10月 東京理科大・野田キャンパスの花めぐり」(アウトリーチ活動)
• [Remarks] 2012.9.20 福岡県立伝習館高等学校出張授業(アウトリーチ活動)
• [Remarks] 2012.8.26 日本バイオイメージング学会公開講座「若い未来の研究者のために」(アウトリーチ活動)
• [Remarks] 2012.8.7 東京理科大学オープンキャンパス実験講座「自分のDNAを見てみよう!～バイオイメージングの世界～」(アウトリーチ活動)
• [Remarks] 2012.6.30 専修大学松戸高等学校出張授業(アウトリーチ活動)
• [Remarks] 2011.11.19-20 東京理科大学サイエンス夢工房「自分のDNAを見てみよう!～バイオイメージングの世界～」 「生きた光るクラゲや, 青いカーネーションを見ながら, 遺伝子・バイオテクノロジーについて考えてみよう!」(アウトリーチ活動)
• [Remarks] 2011.8.8 東京理科大学オープンキャンパス実験講座「自分のDNAを見てみよう!～バイオイメージングの世界～」(アウトリーチ活動)
• [Remarks] 2011.7.2 千葉市理科実験教室「自分のDNA を見てみよう!～バイオイメージングの世界～」(アウトリーチ活動)
• [Remarks] 2011.6.24 千葉県立鎌ヶ谷葛飾高等学校出張授業(アウトリーチ活動)
• [Patent(Industrial Property Rights)] 植物免疫調節剤 (2013)
  • Inventor(s): 朽津和幸, 北畑信隆
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2013-041221
  • Filing Date: 2013-03-01
• [Patent(Industrial Property Rights)] METHOD OF SCREENING FOR PLANT DEFENSE ACTIV A TORS, PLANT DEFENSE ACTIV A TORS, AND METHOD OF ENHANCING IMMUNE RESPONSE (2011)
  • Inventor(s): Kuchitsu, K., Kurusu, T
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCTJP2011/068585
  • Filing Date: 2011-08-31
  • Overseas
• [Patent(Industrial Property Rights)] METHOD OF SCREENING FOR PLANT DEFENSE ACTIV A TORS, PLANT DEFENSE ACTIV A TORS, AND METHOD OF ENHANCING IMMUNE RESPONSE (2010)
  • Inventor(s): Kuchitsu, K., Kurusu, T
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 61/378403
  • Filing Date: 2010-08-31
  • Overseas