Chromosome evolution in Lepidoptera analyzed by comparative FISH mapping

2014 Fiscal Year Final Research Report

• Project/Area Number: 23380030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Insect science
• Research Institution: Iwate University (2012-2014); Hokkaido University (2011)
• Principal Investigator: SAHARA Ken 岩手大学, 農学部, 教授 (30241368)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Go 大阪教育大学, 教育学部, 教授 (10314444)
• Co-Investigator(Renkei-kenkyūsha): YASUKOCHI Yuji 独立行政法人農業生物資源研究所, その他部局等, 研究員 (50355723)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 染色体進化 / FISH / オルソログ / カイコゲノム

Outline of Final Research Achievements

We obtained information of Bombyx mori single orthologs from Plutella xylostella, Endoclita excrescens and Stenopsyche marmorata by means of EST analyses. BAC libraries in the latter two species were also constructed in the present study. The BAC-FISH experimental system in these species were successfully developed to analyze chromosome correspondence to the model Lepidoptera, the silkworm. Comprehensive genome comparison between B. mori and the target species revealed that conserved synteny of gene order is apparent in P. xylostella. While a trichopteran representative, S. marmorata showed only putative microsyntenic correspondence to the gene order of the silkworm. Pieris does not have conserved synteny in spite of well conserved correspondence between the two species.

Free Research Field

昆虫細胞遺伝学