2013 Fiscal Year Final Research Report
Molecular mechanisms for apoptosis induction and suppression n insect cells infected with nucleopolyhedroviruses
| Project/Area Number |
23380034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | アポトーシス / Apsup / Dronc / カスパーゼ / バキュロウイルス / 核多角体病ウイルス / カイコ / マイマイガ |
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| Research Abstract |
Apoptosis serves as the major anti-viral defense mechanism in baculovirus-infected lepidopteran insect cells. In the present study, we examined molecular mechanisms underlying induction and inhibition of apoptosis that occur in insect cells upon infection with nucleopolyhedroviruses (NPVs). We cloned a number of cDNAs of cellular and viral factors, which possibly function in the apoptotic pathways, from several NPVs and insect cells. These include cellular factors Dronc, Caspase-1s, IAPs, P53, Reaper and Sirt2, and viral factors Apsup and IAPs. Through functional analysis of these factors in transient expression experiments, we provide several important insights into regulation mechanisms of apoptosis induction and inhibition in NPV-infected insect cells. We also demonstrated that IAP1 inhibits Dronc activation and promotes degradation of activated Dronc to prevent apoptosis induction in non-apoptotic insect cells.
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[Book] 最新昆虫病理学2014
Author(s)
国見裕久・小林迪弘, ほか 20 名
Total Pages
1-24, 108-116, 182-185
Publisher
講談社
