Arsenic transport system by plant-soil microorganism interaction

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380044
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: National Institute for Agro-Environmental Sciences
• Principal Investigator: ISHIKAWA Satoru 独立行政法人農業環境技術研究所, その他部局等, 研究員 (40354005)
• Co-Investigator(Kenkyū-buntansha): TAKAGI Kazuhiro (独)農業環境技術研究所, 土壌環境研究領域, 上席研究員 (70354074) ; FUJIMAKI Shu (独)日本原子力研究開発機構, 量子ビーム応用研究部門, 研究グループリーダー (20354962) ; SUZUI Nobuo (独)日本原子力研究開発機構, 量子ビーム応用研究部門, 研究副主幹 (20391287)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 無機ヒ素 / メチルヒ素 / イネ根圏 / 土壌微生物

Research Abstract

We isolated from the rice rhizosphere two bacteria responsible for As methylation. Strain GSRB54 and GSRB05 were classified into the genus Streptomyces and Burkholderia, respectively, by 16S rRNA sequencing. The arsenite S-adenosylmethionine methyltransferase (arsM) gene of GSRB54 was phylogenetically different from known arsM genes in other bacteria. Heterologous expression of GSRB54 arsM in E. coli converted the methylation of As(III) into DMA. On the other hand, strain GSRB05 produced an unknown organic As compound (uAs1). The analyses by using LC/MS/MS, 1H, 13C-NMR and 2D-NMR elucidated that the chemical structure of uAs1 is amino acid derivative with a monomethylated As partial structure and we named it as Arsenothricin (AST). AST was degraded into MMA by Arthrobacter sp. These results suggest that the bacteria isolated from this study are associated with methylated As accumulation in rice grains through As methylation in the rice rhizosphere.

Research Products

• [Presentation] Arsenic Dynamics in Rhizosphere and Relevance to Arsenic Speciation in Rice Grains - Arsenic Transformation by Rhizobacteria Separated from Rice Plant-
  • Author(s): Kuramata M, Kataoka R, Abe T, Yamazaki K, Sakakibara F, Asano M, Baba K, Takagi K, Hiradate S, Kamo T, Ishikawa S
  • Organizer: XVII. INTERNATIONAL PLANT NUTRITION COLLOQUIUM
• [Presentation] Rhizobacteria related to arsenic transformation in paddy soil - relevance to methylated arsenic in rice grains -
  • Author(s): Kuramata M, Kataoka R, Abe T, Sakakibara F, Takagi K, Ishikawa S
  • Organizer: ASA, CSSA, and SSSA International Annual Meetings
• [Presentation] Separation and identification of rhizobacteria producing methylated arsenicals in paddy soil - Relevance to arsenic speciation in rice grains -
  • Author(s): Kuramata M, Kataoka R, Abe T, Sakakibara F, Asano M, Baba K, Takagi K, Ishikawa S
  • Organizer: "Risk Alleviation Technologies for Arsenic and Cadmium Contamination of Foods in Monsoon Asia" MARCO Satellite International Symposium 2012 Proceedings
• [Presentation] イネ根圏土壌細菌の生産する新規有機ヒ素化合物の同定と根圏におけるヒ素の化学形態変化について
  • Author(s): 倉俣正人, 片岡良太, 山崎健一, 榊原風太, 安部匡, 馬場浩司, 高木和広, 加茂綱嗣, 平舘俊太郎, 石川覚
  • Organizer: 日本土壌肥料学会 講演要旨集
• [Presentation] イネ根圏微生物が生産する新規ヒ素化合物の同定
  • Author(s): 倉俣正人, 片岡良太, 山崎健一, 榊原風太, 馬場浩司, 石坂眞澄, 安部匡, 高木和広, 加茂綱嗣, 平舘俊太郎, 石川覚
  • Organizer: 第19回ヒ素シンポジウム講演要旨集
• [Presentation] コメに蓄積するヒ素の化学形態と土壌中の動態に関する微生物の影響について
  • Author(s): 倉俣正人, 片岡良太, 榊原風太, 安部匡, 高木和広, 石川覚
  • Organizer: 日本農芸化学2013年度大会
• [Presentation] イネ根圏土壌から分離した微生物のヒ素代謝
  • Author(s): 倉俣正人, 片岡良太, 山崎健一, 榊原風太, 馬場浩司, 石坂眞澄, 安部匡, 高木和広, 加茂綱嗣, 平舘俊太郎, 石川覚
  • Organizer: 第18回ヒ素シンポジウム講演要旨集
• [Presentation] イネの化学形態別ヒ素吸収における土壌微生物の関与
  • Author(s): 倉俣正人, 片岡良太, 安部匡, 井倉将人, 松本真悟, 高木和広, 荒尾知人, 石川覚
  • Organizer: 日本土壌肥料学会 講演要旨集