2013 Fiscal Year Final Research Report
Basic study on the network of mRNA maturation process by analyzing diversified mRNA exporter.
| Project/Area Number |
23380059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MASUDA Seiji 京都大学, 生命科学研究科, 准教授 (20260614)
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| Co-Investigator(Kenkyū-buntansha) |
OKUMURA Katsuzumi 三重大学, 大学院・生物資源学研究科, 教授 (30177183)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | mRNA / 核外輸送 / 輸送体 |
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| Research Abstract |
Messenger RNA exporter regulates the mRNA processing. In human, mRNA exporter is diversified. Here, I am focusing on the mechanism of the complex formation of TREX and AREX as functional mRNA exporters.
The analysis using chimera mutants and point-mutated proteins, I have shown that TREX and AREX complex formation by UAP56 and URH49 are regulated by single amino acid exchange. This indicates that UAP56 and URH49 were diversified from the common ancestor and obtained the specific function through forming specific complex.
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[Journal Article] Cooperative functions of ZnT1, metallothionein and ZnT4 in the cytoplasm are required for full activation of TNAP in the early secretory pathway2013
Author(s)
Fujimoto, S., Itsumura, N., Tsuji, T., Anan, Y., Tsuji, N., Ogra, Y., Kimura, T., Miyamae, Y., Masuda, S., Nagao, M. and Kambe T
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Journal Title
PLoS ONE
Volume: 8
Pages: e77445
DOI
Peer Reviewed
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[Journal Article] Identification of 6-octadecynoic acid from a methanol extract of Marrubium vulgare L. as a peroxisome proliferator-activated receptor γ agonist2013
Author(s)
Ohtera, A., Miyamae, Y., Nakai, N., Kawachi, A., Kawada, K., Han, J., Isoda, H., Neffati, M., Akita, T., Maejima, K., Masuda, S., Kambe, T., Mori, N., Irie, K. and Nagao, M
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 440
Pages: 204-209
DOI
Peer Reviewed
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[Journal Article] Establishment of the monitoring system which detects the inhibition of mRNA processing2012
Author(s)
Fujita, K., Okamura, M., Nishimoto, S., Kurihara, T., Momma, K., Miyamae, Y., Kambe, T., Nagao, M., Narita, H. and Masuda, S
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Journal Title
Biosci. Biotechnol. Biochem
Volume: 76
Pages: 1248-1251
URL
Peer Reviewed
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[Journal Article] Enhancing recombinant protein production in human cell lines with a constitutive transport element and mRNA export proteins2011
Author(s)
Aihara. Y., Fujiwara, N., Yamazaki, T., Kambe, T., Nagao, M., Hirose, Y. and Masuda, S
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Journal Title
J. Biotechnol
Volume: 153
Pages: 86-91
DOI
Peer Reviewed
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[Journal Article] Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway2011
Author(s)
Fukunaka, A., Kurokawa, Y., Teranishi, F., Sekler, I., Oda, K., Ackland, ML., Faundez, V., Hiromura, M., Masuda, S., Nagao, M., Enomoto, S. and Kambe, T
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Journal Title
J Biol Chem
Volume: 286
Pages: 16363-16373
DOI
Peer Reviewed
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