2013 Fiscal Year Final Research Report
Molecular mechanism on the seasonal change in biosynthetic pathways of rose scent compound in response to the environment
| Project/Area Number |
23380063
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | Shizuoka University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
HARA Masakazu 静岡大学, 農学研究科, 教授 (10293614)
OHNISHI Toshiyuki 静岡大学, 農学研究科, 准教授 (60542165)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 生合成 / バラ香気成分 / 環境要因 / 芳香族アミノ酸脱アミノ酵素 / ケト酸脱炭酸酵素 / プロトプラスト / GCMS / LCMS |
|---|
| Research Abstract |
The objective of the research is to clarify the molecular mechanism on the seasonal change in biosynthetic pathways of rose scent 2-phenylethanol (2PE) in response to environment. Based on the feeding experiments of L-[2H8]phenylalanin ([2H8]LPhe) to rose flowers and the GCMS, and LCMS analyses, we have clarified that two new enzymes, AAAT and PPDC are involved in the new biosynthetic pathway from LPhe to 2PE (LPhe, PPA, PAld, 2PE). We have also confirmed that the pathway is actively operating under the environmental conditions at high temperature.
|
-
[Journal Article] Molecular cloning and characterization of a short chain dehydrogenase showing activity with volatile compounds isolated from Camellia sinensis2014
Author(s)
Ying Zhou, Ling Zhang, Jiadong Gui, Fang Dong, Sihua Cheng, Xin Mei, Linyun Zhang, Yongqing Li, Xinguo Su, Susanne Baldermann, Naoharu Watanabe, Ziyin Yang
-
Journal Title
Plant Molecular Biology Reporter
DOI
Peer Reviewed
-
[Journal Article] Characterization of odorant compounds and their biochemical formation in green tea with a low temperature storage process2014
Author(s)
Tsuyoshi Katsuno, Hisae Kasuga, Yumi Kusano, Yoshihiro Yaguchi, Miho Tomomura, Jilai Cui, Ziyin Yang, Susanne Baldermann, Yoriyuki Nakamura, Toshiyuki Ohnishi, Nobuyuki Mase, Naoharu Watanabe
-
Journal Title
Food Chem
Volume: 148
Pages: 388-395
DOI
Peer Reviewed
-
[Journal Article]2013
Author(s)
Ariaki Murata, Ulrich H. Engelhardt, Peter Fleischmann, Keita Yamada, Naohiro Yoshida, Dieter Juchelka, Andreas Hilkert, Toshiyuki Ohnishi, Naoharu Watanabe, Peter Winterhalter
-
Journal Title
J. Agric. Food Chem
Volume: 61
Pages: 11321-11325
DOI
Peer Reviewed
-
[Journal Article] Characterization of volatile and non-volatile metabolites in etiolated leaves of tea (Camellia sinensis) plants in the dark2012
Author(s)
Ziyin Yang, Eiji Kobayashi, Tsuyoshi Katsuno, Toshimichi Asanuma, Tamaki Fujimori, Takamasa Ishikawa, Miho Tomomura, Kazuo Mochizuki, Takaya Watase, Yoriyuki Nakamura, Naoharu Watanabe
-
Journal Title
Food Chem
Volume: 135
Pages: 2268-2276
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
