Chemical Ecology of Mycorrhizal Matsutake

2013 Fiscal Year Final Research Report

• Project/Area Number: 23380083
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Forest science
• Research Institution: Kyoto University
• Principal Investigator: HIRAI Nobuhiro 京都大学, (連合)農学研究科(研究院), 教授 (00165151)
• Co-Investigator(Kenkyū-buntansha): YAMADA Akiyoshi 信州大学, 農学部, 准教授 (10324237) ; TANAKA Chihiro 京都大学, 大学院・農学研究科, 教授 (60263133)
• Research Collaborator: FUJITA Thoru 京都府農林水産技術センター ; YOSHIKAWA Masami 京都府農林水産技術センター
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 森林生態 / 保護 / 保全 / 菌根

Research Abstract

Matsutake is a typical ectomycorrhizal fungus associated with roots of red pine, and forms a fairy ring of fruiting bodies. The area of the fairy ring contains active mycorrhizas, and called as shiro. The previous study reported that that the shiro contained an antimicrobial substance. The antimicrobial substance has been remained unknown for 47 years. Our objective is isolation and identification of the antimicrobial substance from the shiro.
The shiro was extracted with 20% methanol, and the extract was purified with ODS and anion exchange resin to give an active fraction. The active fraction gave a crystalline, which was identified as oxalic acid by an X-ray analysis. However, the antimicrobial activity of the active fraction was clearly higher than that of oxalic acid. This means that the active fraction contains another substance which increases the activity of oxalic acid. The NMR and elemental analysis suggested that the substance was an inorganic compound.

Research Products

• [Journal Article] Synthesis of Japanese Boletus edulis ectomycorrhizae with Japanese red pine (2014)
  • Author(s): Endo, N., Kawamura, F., Kitahara, R., Sakuma, D., Fukuda, M., Yamada, A
  • Journal Title: Mycoscience Volume: (in press)
• [Journal Article] Transformation of the mushroom species Hypsizigus marmoreus, Flammulina velutipes, and Grifola rondosa by an Agrobacterium-mediated method using a universal transformation plasmid (2013)
  • Author(s): Hatoh, K., Izumitsu, K., Morita, A., Shimizu, K., Ohta, A., Kawai, M., Yamanaka, T., Neda, H., Ota, Y., Tanaka, C
  • Journal Title: Mycoscience Volume: 54 Pages: 8-12
  • DOI: 10.1016/j.myc.2012.08.00215
• [Journal Article] Trichoroma matsutake Y1 strain associated with Pinus densiflorae shows a gradient of in vitro ectomycorrhizal specificity with Pinaceae and oak hosts (2013)
  • Author(s): Yamada, A., Endo, N., Murata, H., Ohta, A., Fukuda, M
  • Journal Title: Mycoscience Volume: 55 Pages: 27-34
  • DOI: 10.1016/j.myc.2-13.05.004
• [Journal Article] Phylogenetic relationship and species delimitation of matsutake and allied species based on multilocus phylogeny and haplotype analyses (2012)
  • Author(s): Ota, Y., Yamanaka, T., Murata, H., Neda, H., Ohta, A., Kawai, M., Yamada, A., Konno, M., Tanaka, C
  • Journal Title: Mycologia Volume: 104 Pages: 1369-1380
  • DOI: 10.3852/12-068
• [Journal Article] A sheathing mycorrhiza between the tropical bolete Phlebopus spongiosus and Citrus maxima (2012)
  • Author(s): Phan, NDH., Yamada, A., Shimizu, K., Noda, K., Dang, LAT., Suzuki, A
  • Journal Title: Mycoscience Volume: 52 Pages: 347-353
  • DOI: 10.1007/s10267-011-0177-5
• [Journal Article] Ectomycorrhizal formation on regenerated somatic pine plants after inoculation with Tricholoma matsutake (2012)
  • Author(s): Yamanaka, T., Maruyama, T., Yamada, A., Miyazaki, Y., Kikuchi, T
  • Journal Title: MushroomScience and Biotechnology Volume: 20 Pages: 93-97
• [Journal Article] Dehydration tolerance in apple seedlings is affected by an inhibitor of ABA 8'-hydroxylase CYP707A. Dehydration tolerance in apple seedlings is affected by an inhibitor of ABA 8'-hydroxylase CYP707A (2012)
  • Author(s): Kondo, S., Sugaya, S., Sugawa, S., Ninomiya, M., Kittikorn, M., Okawa, K., Ohara H.,, Ueno, K., Todoroki, Y., Mizutani, M., Hirai, N
  • Journal Title: J. Plant Physiol Volume: 169 Pages: 234-24
  • DOI: 10.1016/j.jplph.2011.09.007
• [Journal Article] Synthesis and biological activity of amino acid conjugates of abscisic acid (2011)
  • Author(s): Todoroki, Y., Narita, K., Muramatsu, T., Shimomura, H., Ohnishi, T., Mizutani, M., Ueno, K., Hirai, N
  • Journal Title: Bioorganic and Medicinal Chemistry Volume: 19 Pages: 1743-1750
  • DOI: 10.1016/j.bmc.2011.01.019
• [Journal Article] Six-year monitoring of pine ectomycorrhizal biomass under a temperate monsoon climate indicates significant annual fluctuations in relation to climatic factors (2011)
  • Author(s): Okada, K., Okada, S., Yasue, K., Fukuda, M., Yamada, A
  • Journal Title: Ecological Research Volume: 26 Pages: 411-419
  • DOI: 10.1007/s11284-011-0800-0
• [Journal Article] Molecular organization of the mating type (Mat) locus of Exserohilummonoceras (Setosphaeria monoceras), a bioherbicide agent for Echinochloa weeds (2011)
  • Author(s): Morita, A, . Saitoh, Y., Izumitsu, K., Tanaka, C
  • Journal Title: Mycoscience Volume: 53 Pages: 92-101
  • DOI: 10.1007/s10267-011-0141-4
• [Presentation] アカマツ菌根圏土壌の抗菌活性物質 (2014)
  • Author(s): 西野勝俊, 城美沙緒, 大泉一也, 藤田徹, 山田明義, 田中千尋, 笹森貴裕, 時任宣博, 平井伸博
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学(生田キャンパス)
  • Year and Date: 2014-03-30
• [Presentation] 灰色かび病菌のオートファジー関連遺伝子BcAtg1の機能解析 (2013)
  • Author(s): 住田卓也, 泉津弘佑, 田中千尋
  • Organizer: 第13回糸状菌分子生物学コンファレンス
  • Place of Presentation: つくば国際会議場
  • Year and Date: 20131120-21
• [Presentation] マツタケ単一子実体由来の胞子分離株集団の選抜と培養特性 (2013)
  • Author(s): 早川記央, 村田仁, 山田明義
  • Organizer: 日本菌学会第57回大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 20130607-09
• [Presentation] Fruit-body formation of edible mycorrhizal Cantharellus from Japan in pot culture with pine host (2013)
  • Author(s): Ogawa, W., Endo, N., Yamada, A
  • Organizer: 7th International Workshop of Edible Ectomycorrhizal Mushroom
  • Year and Date: 2013-07-31
• [Presentation] 植木鉢を用いたマツタケ菌根苗順化の試み (2012)
  • Author(s): 小林 久泰, 山田明義
  • Organizer: 日本きのこ学会
  • Place of Presentation: 東京都, 東京農業大学
  • Year and Date: 2012-09-06
• [Presentation] マツタケ研究における菌根合成法の展開 (2011)
  • Author(s): 山田明義
  • Organizer: 日本きのこ学会
  • Place of Presentation: 信州大学農学部
  • Year and Date: 2011-09-02
  • Invited
• [Book] 菌類の事典 基礎編VI 生態15.3.3 菌類間の共生 (2013)
  • Author(s): 大園享司, 田中千尋
  • Publisher: 朝倉書店