2013 Fiscal Year Final Research Report
Development of novel methods of induction of sexual maturation in aquaculture fishes using recombinant gonadotropins
| Project/Area Number |
23380116
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | International Christian University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAGAWA Hirohiko 宮崎大学, 農学部, 教授 (60169381)
SOYANO Kiyoshi 長崎大学, 水産・環境科学総合研究科(環シナ), 教授 (80260735)
YOSHIZAKI Goro 東京海洋大学, 海洋科学部, 准教授 (70281003)
NUNOSHIBA Tatsuo 国際基督教大学, 教養学部, 教授 (10270802)
HAYAKAWA Yoichi 国際基督教大学, アーツ・サイエンス学科, 研究員 (50384011)
YOSHIZAKI Goro 東京海洋大学, 海洋科学部, 教授 (70281003)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 生殖腺刺激ホルモン / 組換えホルモン / バイオテクノロジー / 魚類 / 性成熟促進 / 養殖 |
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| Research Abstract |
Knowledge of reproductive endocrinology of fish is essential for the efficient aquaculture. However, information on the function of pituitary gonadotropin of fish is not clearly known because of unavailability of the hormones. Therefore, we started a project to produce recombinant fish gonadotropins biotechnologically using silkworm larvae as a host and distribute the hormones to fish biologists for their research. In the present study, we investigated the efficiency of the hormone production, method of hormone purification, stability of administered hormones in fish, biological activities of hormones, and sensitivity to the hormones in recipient fish.
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[Journal Article] Mechanism of oocyte matuation and ovulation and its application to seed production in the Japanese eel2013
Author(s)
Kagawa, H., Sakurai, Y., Horiuchi, R., Kazeto, Y., Gen, K., Imaizumi, H., Masuda, Y
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Journal Title
Fish Physiol. Biochem
Volume: 39
Pages: 13-17
Peer Reviewed
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[Journal Article] Using osmotic pumps to deliver hormones to induce sexual maturation of female Japanese eels2013
Author(s)
Kagawa, H., Fujie, N., Imaizumi, H., Masuda, Y., Oda, K., Adachi, J., Nishi, A., Hashimoto, H., Teruya, K., Kaji, S
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Journal Title
Anguilla japonica. Aquaculture
Volume: 388
Pages: 30-34
Peer Reviewed
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[Journal Article] Experimental and computational study of the inter- and intra-species specificity of gonadotropins for various gonadotropin receptors2012
Author(s)
Aizen, J., Kowalsman, N., Kobayashi, M., Hollander, L., Sohn, Y.C., Yoshizaki, G., Niv, M. Y., and Levavi-Sivan, B
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Journal Title
Mol. Cell. Endocrinol
Volume: 364
Pages: 89-100
Peer Reviewed
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[Journal Article] Steroidogenic response of carp ovaries to piscine FSH and LH depends on the reproductive phase2012
Author(s)
Aizen, J., Kobayashi, M., Selicharova, H., Sohn, Y.C., Yoshizaki, G., and Levavi-Sivan, B
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Journal Title
Gen. Comp. Endocrinol
Volume: 178
Pages: 28-36
Peer Reviewed
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[Presentation] Influence of age and growth on pubertal development in Epinephelus bruneus2013
Author(s)
Yamamoto, K., Nakagawa, M., Yoshida, K., Hotta, T., Tsusaki, T., Izumida, D., Soyano, K
Organizer
The 10th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
Place of Presentation
Jeju National University, Jeju, Korea
Year and Date
20131118-21
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[Presentation] The biological information of final maturation in the white-streaked grouper2013
Author(s)
Yamaguchi, T., Tawaki, S., Kawabata, Y., Takebe, T., Nanami, A., Sato, T., Teruya, K., Soyano, K
Organizer
The 10th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
Place of Presentation
Jeju National University, Jeju, Korea
Year and Date
20131118-21
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