Food safety assessment of GH transgenic amago based on gene expression profiles and metabolic analysis.

2014 Fiscal Year Final Research Report

• Project/Area Number: 23380117
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Nihon University
• Principal Investigator: MORI Tsukasa 日本大学, 生物資源科学部, 教授 (60241379)
• Co-Investigator(Kenkyū-buntansha): SEKI Taiichro 日本大学, 生物資源科学部, 教授 (20187834)
• Co-Investigator(Renkei-kenkyūsha): ARAKI Kazuo 独立行政法人水産総合研究センター, その他部局等, 研究員 (00202739)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 遺伝子組換え / GH（成長ホルモン） / アマゴ / 発現遺伝子 / 代謝産物

Outline of Final Research Achievements

GH transgenic Amago were compared the FAs composition and content in the liver using gas chromatography. Eleven kinds of FA were detected. The composition of SFA and MUFA such as 14:0, 16:1n-7, and cis-18:1n-7 was significantly decreased in GH transgenic Amago. On the other hand, the composition of PUFAs such as 18:2n-6, 20:4n-6, and 22:5n-3 was significantly increased. Levels of serum glucose and triacylglycerol were significantly decreased in the GH transgenics compared with +/+ fish. Furthermore, 3’-tag digital gene expression profiling was performed using liver tissues from Tg/Tg and +/+ fish, and showed that Mid1ip1, which is an important factor to activate ACC, was down-regulated in Tg/Tg fish, while genes involved in FA catabolism were up-regulated, including ACSL1 and ACOX3. These data suggest that liver tissue from GH transgenic Amago showed starvation by alteration in glucose and lipid metabolism due to GH overexpression.

Free Research Field

分子生理学