Analysis of biological properties of breeding streptococcul infection disease resistance flounder

2014 Fiscal Year Final Research Report

• Project/Area Number: 23380119
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Fisheries Research Agency
• Principal Investigator: ARAKI Kazuo 独立行政法人水産総合研究センター, 増養殖研究所, 主幹研究員 (00202739)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Hiroyuki 独)水産総合研究センター増養殖研究所, 育種研究センター・基盤研究グループ, 主任研究員 (50372040) ; OKAUCHI Masanori 独)水産総合研究センター増養殖研究所, 育種研究センター・基盤研究グループ, 主幹研究員 (40372023) ; OZAKI Terumichi 独)水産総合研究センター増養殖研究所, 育種研究センター・基盤研究グループ, 主幹研究員 (40416045)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: ヒラメ / 連鎖解析 / 物理地図 / レンサ球菌 / 自然免疫 / 獲得免疫 / TRL2

Outline of Final Research Achievements

We analyzed disease resistance trait and developed the physical map of flounder. The streptococcul infection disease resistant flounder increased monocyte and granulocyte dramatically after infection. One the other side, the native immunity system of streptococcul infection sensitive flounder did not respond against streptococcul infection. We detected TRL21 and NKEF genes in the region of linkage group 10 contributed to streptococcul infection disease resistance trail by detailing flounder physical map. And, we analyzed the sequences of TRL21 and NKEF genomic genes of the resistance, sensitive and famed flounder. We found single nucleotide polymorphism in regulatory site of TRL21 gene of resistance flounder and deletion between ninth exon and intron of TRL21 in sensitive flounder.

Free Research Field

分子生物学