Analyses of myelin repair/regeneration for establishment of therapeutic strategy using the myelin mutant rat

2014 Fiscal Year Final Research Report

• Project/Area Number: 23380180
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: KUWAMURA Mitsuru 大阪府立大学, 生命環境科学研究科(系), 准教授 (20244668)
• Co-Investigator(Kenkyū-buntansha): YAMATE Jyoji 大阪府立大学, 生命環境科学研究科, 教授 (50150115)
• Co-Investigator(Renkei-kenkyūsha): KURAMOTO Takashi 京都大学, 医学部, 准教授 (20311409)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 脱髄 / ミエリン / オリゴデンドロサイト / ミトコンドリア / 多発性硬化症 / モデル動物 / 再生

Outline of Final Research Achievements

We identified a nonsense mutation in the Dopey1. We also demonstrated a marked reduction in myelin components both at mRNA and protein levels during myelinogenesis in the VF rats. In addition, proteolipid protein and myelin-associated glycoprotein accumulated in oligodendrocyte cell body. Dopey1 is likely to be involved in the traffic of myelin components. Our results highlighted the importance of Dopey1 for the development and maintenance of the CNS myelin.　We examined transcription factors (PDGFRa，Olig2，Nkx2.2) in the dmy rat. These factors were reduced in the affected dmy rat, suggesting impaired development, maturation and function in the oligodendrocyte.

Free Research Field

獣医病理学