2013 Fiscal Year Final Research Report
Osteochondral regeneration with scaffold free 3D-structure of swine adipose tissue-derived mesenchymal stem cells.
| Project/Area Number |
23380185
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Clinical veterinary science
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUJIKI Makoto 鹿児島大学, 共同獣医学部, 准教授 (60305167)
NAKAYAMA Kouichi 佐賀大学, 工学系研究科(研究院), 教授 (50420609)
KAWAGUCHI Hiroaki 鹿児島大学, 共同獣医学部, 准教授 (60325777)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 外科 / 幹細胞 / 再生医療 / 関節 / 骨 / 軟骨 |
|---|
| Research Abstract |
This study was designed to investigate the regeneration of articular cartilage and subchondral bone using a three-dimensional (3D) construct of adipose tissue-derived mesenchymal stem cells (AT-MSCs). A construct consisting of about 760 spheroids each containing 50,000 autologous AT-MSCs was implanted into an osteochondral defect at the femoral trochlear groove. In CT images after surgery, a radiopaque area emerged from the boundary between the bone and the implant and increased more steadily upward and inward in the implants. Histopathology of the implants after 6 months revealed active endochondral ossification underneath the plump fibrocartilage. Histopathology after 12 months showed that not only was the diminishing fibrocartilage as thick as the surrounding normal cartilage, but massive subchondral bone was also observed. We supposed that implantation of a scaffold-free 3D construct of AT-MSCs will induce regeneration of original structure of cartilage and subchondral bone.
|
-
-
[Presentation] Osteochondral regeneration with scaffold free 3D-structureof swine adipose tissue-derived mesenchymal stem cells
Author(s)
Murata, D., Tokunaga, S., Tamura, T., Kawaguchi, H., Fujiki, M., Miyoshi, N., Nakayama, K., Misumi, K
Organizer
International Cartilage Repair Society
Place of Presentation
Izmir, Turkey
-
-
-
-
