2013 Fiscal Year Final Research Report
Escherichia coli host engineering for non-biased expression of metagenomic library
| Project/Area Number |
23380197
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
MIYAZAKI Kentaro 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (60344123)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | メタゲノム / バイオテクノロジー / 大腸菌 / ゲノム育種 / リボソーム工学 |
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| Research Abstract |
We have applied translational engineering approach to solve the problem in heterologous expression problem in Escherichia coli. Initiation factor 3 as well as the ribosome was engineered to alter the translational profile and variants that showed enhanced expression of genes having non-AUG initiation codon were identified. Also, mutant ribosome that have 16S rRNA from non-E. coli bacteria showed altered, or enhanced expression of reporter gfp genes, that were not efficiently expressed in wild type E. coli.
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[Journal Article] Meeting Report : 1st International Functional Metagenomics Workshop2013
Author(s)
Engel K, Ashby D, Brady SF, Cowan DA, Doemer J, Edwards EA, Fiebig K, Martens EC, McCormac D, Mead DA, Miyazaki K, Moreno-Hagelsieb G, O'Gara F, Reid A, Rose DR, Simonet P, Sjöling S, Smalla K, Streit WR, Tedman-Jones J, Valla S, Wellington EMH, Wu C-C, Liles MR, Neufeld JD, Sessitsch A, Charles TC
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Journal Title
St. Jacobs, Ontario, Canada. Stand Genomic Sci
Volume: 8
Pages: 106-111
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