2013 Fiscal Year Final Research Report
Development of novel cancer therapy using miRNA containing exosome
| Project/Area Number |
23390087
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OCHIYA Takahiro 独立行政法人国立がん研究センター, 研究所, 分野長 (60192530)
SUDO Katsuko 東京医科大学, 医学部, 兼任講師 (50126091)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | エクソソーム / microRNA / EGFR / 分子標的治療 |
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| Research Abstract |
Despite the therapeutic potential of nucleic acid drugs, their clinical application has been limited in part by a lack of appropriate delivery systems. Exosomes or microvesicles are small endosomally derived vesicles that are secreted by a variety of cell types and tissues. Here, we show that exosomes can efficiently deliver microRNA to epidermal growth factor receptor (EGFR)-expressing breast cancer cells. Targeting was achieved by engineering the donor cells to express the transmembrane domain of platelet-derived growth factor receptor fused to the GE11 peptide. Intravenously injected exosomes delivered let-7a microRNA to EGFR-expressing xenograft breast cancer tissue in Rag2-/- mice. Furthermore, we developed mimic miRNA that have efficient loading activity of the RISC complex. Our results suggest that exosomes can be used therapeutically to target EGFR-expressing cancerous tissues with nucleic acid drugs.
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