Development of risk assessment methods for parkinson's disease using carbonylated protein, a protein oxidation product, as a predictive indicator

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390162
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Mie University
• Principal Investigator: OIKAWA Shinji 三重大学, 医学(系)研究科(研究院), 准教授 (10277006)
• Co-Investigator(Kenkyū-buntansha): TOMIMOTO Hidekazu 三重大学, 大学院・医学系研究科, 教授 (80324648) ; MURATA Mariko 三重大学, 大学院・医学系研究科, 教授 (10171141) ; YAMASHIMA Tetsumori 金沢大学, 医学系, 准教授 (60135077)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: パーキンソン病 / 酸化ストレス / プロテオミクス解析 / 酸化損傷タンパク質 / バイオマーカー

Research Abstract

Oxidative stresses cause cell death of dopaminergic neurons of the substantia nigra (SN) in the brain of Parkinson's disease (PD). Therefore, we performed the identification and characterization of carbonylated protein, a protein oxidation product, in plasma of PD patients by using two-dimensional gel electrophoresis with immunochemical detection of protein carbonyls (2D Oxyblot). The specific oxidation level of complement component C9, vitamin D-binding protein, serum amyloid P-component, alpha-1-antichymotrypsin and fibrinogen beta chain were increased in plasma of PD patients, compared to the control. Therefore, some of these carbonylated proteins may serve as potential biomarkers helpful in the diagnosis of PD.

Research Products

• [Journal Article] Proteomic analysis of carbonylated proteins in the monkey substantia nigra after ischemia-reperfusion (2014)
  • Author(s): Oikawa S, Kobayashi H, Kitamura Y, Zhu H, Obata K, Minabe Y, Dazortsava M, Ohashi K, Tada-Oikawa S, Takahashi H, Yata K, Murata M, Yamashima T
  • Journal Title: Free Radic Res
  • Peer Reviewed
• [Journal Article] Nile Blue Can Photosensitize DNA Damage through Electron Transfer (2014)
  • Author(s): Hirakawa K, Ota K, Hirayama J, Oikawa S, Kawanishi S
  • Journal Title: Chem Res Toxicol
  • Peer Reviewed
• [Journal Article] Relationships of alpha-SMA-positive fibroblasts and SDF-1-positive tumor cells with neoangiogenesis in nasopharyngeal carcinoma (2014)
  • Author(s): Wang S, Ma N, Kawanishi S, Hiraku Y, Oikawa S, Xie Y, Zhang Z, Huang G, Murata M
  • Journal Title: BioMed Research International
  • Peer Reviewed
• [Journal Article] Expression of proteins associated with adipocyte lipolysis was significantly changed in the adipose tissues of the obese spontaneously hypertensive/NDmcr-cp rat (2014)
  • Author(s): Chang J, Oikawa S, Iwahashi H, Kitagawa E, Takeuchi I, Yuda M, Aoki C, Yamada Y, Ichihara G, Kato M, Ichihara S
  • Journal Title: Diabetol Metab Syndr Volume: 6(1) Pages: 8
  • DOI: 10.1186/1758-5996-6-8
  • Peer Reviewed
• [Journal Article] Inflammation-related DNA damage and expression of CD133 and Oct3/4 in cholangiocarcinoma patients with poor prognosis (2013)
  • Author(s): Thanan R, Pairojkul C, Pinlaor S, Khuntikeo N, Wongkham C, Sripa B, Ma N, Vaeteewoottacharn K, Furukawa A, Kobayashi H, Hiraku Y, Oikawa S, Kawanishi S, Yongvanit P, Murata M
  • Journal Title: Free Radic Biol Med Volume: 65 Pages: 1464-1472
  • DOI: 10.1016/j.freeradbiomed.2013.07.034
  • Peer Reviewed
• [Journal Article] Altered gene and protein expression in liver of the obese spontaneously hypertensive/NDmcr-cp rat (2012)
  • Author(s): Chang J, Oikawa S, Ichihara G, Nanpei Y, Hotta Y, Yamada Y, Tada-Oikawa S, Iwahashi H, Kitagawa E, Takeuchi I, Yuda M, Ichihara S
  • Journal Title: Nutr Metab (Lond) Volume: 9(1) Pages: 87
  • DOI: 10.1186/1743-7075-9-87
  • Peer Reviewed
• [Journal Article] Proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure (2012)
  • Author(s): Huang Z, Ichihara S, Oikawa S, Chang J, Zhang L, Subramanian K, Mohideen SS, Ichihara G
  • Journal Title: Toxicol Appl Pharmacol Volume: 263(1) Pages: 44-52
  • DOI: 10.1016/j.taap.2012.05.021
  • Peer Reviewed
• [Journal Article] Nuclear Localization of COX-2 in relation to the Expression of Stemness Markers in Urinary Bladder Cancer (2012)
  • Author(s): Thanan R, Murata M, Ma N, Hammam O, Wishahi M, El Leithy T, Hiraku Y, Oikawa S, Kawanishi S
  • Journal Title: Mediators Inflamm Pages: 165879.
  • DOI: 10.1155/2012/165879
  • Peer Reviewed
• [Journal Article] Promoter hypermethylation of Ras-related GTPase gene RRAD inactivates a tumor suppressor function in nasopharyngeal carcinoma (2012)
  • Author(s): Mo Y, Midorikawa K, Zhang Z, Zhou X, Ma N, Huang G, Hiraku Y, Oikawa S, Murata M
  • Journal Title: Cancer Lett Volume: 323(2) Pages: 147-154
  • DOI: 10.1016/j.canlet.2012.03.042
  • Peer Reviewed
• [Journal Article] Proton pump inhibitors suppress iNOS-dependent DNA damage in Barrett's esophagus by increasing Mn-SOD expression (2012)
  • Author(s): Thanan R, Ma N, Iijima K, Abe Y, Koike T, Shimosegawa T, Pinlaor S, Hiraku Y, Oikawa S, Murata M, Kawanishi S
  • Journal Title: Biochem Biophys Res Commun Volume: 421(2) Pages: 280-285
  • Peer Reviewed
• [Journal Article] Inflammation-induced protein carbonylation contributes to poor prognosis for cholangiocarcinoma (2012)
  • Author(s): Thanan R, Oikawa S, Yongvanit P, Hiraku Y, Ma N, Pinlaor S, Pairojkul C, Wongkham C, Sripa B, Khuntikeo N, Kawanishi S, Murata M
  • Journal Title: Free Radic Biol Med Volume: 52(8) Pages: 1465-1472
  • Peer Reviewed
• [Journal Article] Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosoma haematobium infection (2011)
  • Author(s): Ma N, Thanan R, Kobayashi H, Hammam O, Wishahi M, El Leithy T, Hiraku Y, Amro el-K, Oikawa S, Ohnishi S, Murata M, Kawanishi S
  • Journal Title: Biochem Biophys Res Commun Volume: 414(2) Pages: 344-349
  • Peer Reviewed
• [Journal Article] Proteomic analysis of hippocampal proteins of F344 rats exposed to 1-bromopropane (2011)
  • Author(s): Huang Z, Ichihara S, Oikawa S, Chang J, Zhang L, Takahashi M, Subramanian K, Mohideen SS, Wang Y, Ichihara G
  • Journal Title: Toxicol Appl Pharmacol Volume: 257(1) Pages: 93-101
  • Peer Reviewed
• [Journal Article] Astrocytic neuroprotection through induction of cytoprotective molecules; a proteomic analysis of mutant P301S tau-transgenic mouse (2011)
  • Author(s): Yata K, Oikawa S, Sasaki R, Shindo A, Yang R, Murata M, Kanamaru K, Tomimoto H
  • Journal Title: Brain Res Volume: 1410 Pages: 12-23
  • Peer Reviewed
• [Journal Article] Proteomic identification of hippocampal proteins vulnerable to oxidative stress in excitotoxin-induced acute neuronal injury (2011)
  • Author(s): Furukawa A, Kawamoto Y, Chiba Y, Takei S, Hasegawa-Ishii S, Kawamura N, Yoshikawa K, Hosokawa M, Oikawa S, Kato M, Shimada A
  • Journal Title: Neurobiol Dis Volume: 43(3) Pages: 706-714
  • Peer Reviewed
• [Presentation] パーキンソン病患者の血漿中カルボニル化タンパク質の同定 (2014)
  • Author(s): 北村祐貴, 都築政弘, 佐々木良元, 市原佐保子, 松永誠治郎, 水野章, 村田真理子, 冨本秀和, 及川伸二
  • Organizer: 第84回日本衛生学会学術総会
  • Place of Presentation: 岡山コンベンションセンター(岡山市)
  • Year and Date: 20140525-27
• [Presentation] Proteomic analysis of carbonylated proteins in the substantia nigra of Japanese monkey after ischemia-reperfusion (2014)
  • Author(s): Yuki Kitamura, Tetsumori Yamashima, Mariko Murata, Shinji Oikawa
  • Organizer: SFRRI 2014 (17th Biennial Meeting of the Society for Free Radical Research International)
  • Place of Presentation: 京都国際会館(京都市)
  • Year and Date: 20140323-26
• [Remarks]
  • URL: http://www.medic.mie-u.ac.jp/eiseigaku/