2013 Fiscal Year Final Research Report
Application of Fourier transformation-linear ion trap mass spectrometers to forensic medicine
| Project/Area Number |
23390183
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
ISHII Akira 名古屋大学, 医学(系)研究科(研究院), 教授 (30252175)
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| Co-Investigator(Kenkyū-buntansha) |
ZAITSU Kei 名古屋大学, 医学系研究科, 准教授 (30700546)
NASU Tamie 中部大学, 生命健康科学部, 教授 (10020794)
HANAJIRI Ruri (KIKURA Ruri) 国立医薬品食品衛生研究所, 生薬部, 室長 (10224916)
SENO Hiroshi 愛知医科大学, 医学部, 教授 (50236113)
HATTORI Hideki 愛知医科大学, 医学部, 准教授 (30107817)
OGAWA Tadashi 愛知医科大学, 医学部, 助教 (50559937)
KANEKO Rina 名古屋大学, 医学系研究科, 助教 (70367697)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 法中毒学 / 質量分析 / オービトラップ / 飛行時間型質量分析計 / 自然毒 / アマニチン / 合成カンナビノイド / ミフェプリストン |
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| Research Abstract |
The purpose of the project is to develop application methods for identifying and quantitating various drugs and toxins using a Fourier transformation-linear ion trap mass spectrometer, shared in the Faculty. However, under the available LC conditions optimized to proteomic analysis, sufficient separation and reproducibility were not obtained. We thus purchased a quadrupole-time of flight mass spectrometer (Q-TOFMS); further experiments were rendered using this (liquid chromatography) LC-Q-TOFMS system.
Using the LC-Q-TOFMS system, amanitins in urine samples were sufficiently separated; the calibration curves showed good linearity in the range of 1 - 5,000 ng/ml. This system has enabled sensitive detection and identification of various synthetic cannabinoids and mifepristone, and their metabolites with high sensitivity. We are now constructing a screening system of 53 natural toxins. Hence, LC-Q-TOFMS will be a useful tool in analyzing various compounds in forensic toxicology.
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[Journal Article] Metabolism of the newly encountered drug α-pyrrolidino- valerophenone in humans : identification and quantitation of urinary metabolites2014
Author(s)
Shima N, Katagi M, Kamata H, Matsuta S, Sasaki K, Kamata T, Nishioka H, Miki A, Tastuno M, Zaitsu K, Ishii A, Sato T, Tsuchihashi H, Suzuki K
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Journal Title
Forensic Toxicol
Volume: 32
Pages: 59-67
Peer Reviewed
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[Journal Article] Mass spectrometric differentiation of the isomers of mono-methoxyethylamphetamines and monomethoxydimethylamphetamines by GC/MS/MS2013
Author(s)
Zaitsu K, Miyagawa H, Sakamoto Y, Matuta S, Tsuboi K, Nishioka H, Katagi M, Sato T, Tatsuno M, Tsuchihashi H, Suzuki K, Ishii A
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Journal Title
Forensic Toxicol
Volume: 31
Pages: 292-300
Peer Reviewed
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[Journal Article] High-fat-cholesterol diet-induced mainly necrosis fibrotic steatohepatities rats by suppressing caspase activity2013
Author(s)
Yetti H, Naito H, Jia X, Shindo M, Taki H, Tamada H, Kitamori K, Hayashi Y, Ikeda K, Yamori Y, Nakajima T
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Journal Title
Life Sciences
Volume: 93
Pages: 673-680
Peer Reviewed
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[Journal Article] The modulation of hepatic adenosine triphosphate and inflammation by eicosapentaenoic acid during severe fibrotic progression in the SHRSP5/Dmcr rat model2012
Author(s)
Jia X, Naito H, Yetti H, Tamada H, Kitamori K, Hayashi Y, Yamagishi N, Wang D, Yanagiba Y, Ito Y, Wang J, Tanaka N, Ikeda K, Yamori Y, Nakajima T
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Journal Title
Life Sci
Volume: 90(23-24)
Pages: 934-43
Peer Reviewed
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[Journal Article] Tamada H, Gonzalez FJ, Nakajima T. Ammonium perfluorooctanoate may cause testosterone reduction by adversely affecting testis in relation to PPARα2011
Author(s)
Li Y, Ramdhan DH, Naito H, Yamagishi N, Ito Y, Hayashi Y, Yanagiba Y, Okamura A, Tamada H, Gonzalez FJ, Nakajima T
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Journal Title
Toxicol Lett
Volume: 205
Pages: 265-72
Peer Reviewed
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[Presentation] High-resolution mass spectrometry and accurate mass measurement of synthetic cannabinoids and their metabolites in human biological specimens by LC/Q-TOFMS2013
Author(s)
Zaitsu K, Yoshimoto T, Yamamoto T, Ogawa T, Hattori H, Matsumoto T, Hisatsune K, Katagi M, Tsuchihashi H, Ishii A
Organizer
The 51st Annual Meeting of the International Association of Forensic Toxicologists
Place of Presentation
Funchal, Portugal
Year and Date
2013-09-05
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[Presentation] LC-Q-TOF-MSによる生体試料中合成カンナビノイド及び代謝物の分析2013
Author(s)
財津桂, 吉本高士, 山本敏充, 鎌田徹, 片木宗弘, 小川匡之, 服部秀樹, 佐藤貴子, 土橋 均, 鈴木廣一, 石井晃
Organizer
日本法中毒学会第32年会
Place of Presentation
さわやかちば県民プラザ 柏市
Year and Date
2013-07-06
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