The development of culture technology of pituitary stem cells using the microgravity environment - with the aim of application to artificial pituitary

2015 Fiscal Year Final Research Report

• Project/Area Number: 23390350
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Hiroshima University
• Principal Investigator: Kurisu kaoru 広島大学, 医歯薬保健学研究院, 教授 (70201473)
• Co-Investigator(Kenkyū-buntansha): YUGE RUI 広島大学, 医歯薬保健学研究院, 教授 (20263676) ; HAMA SEIJI 広島大学, 医歯薬保健学研究院, 研究員 (40397980) ; TOMINAGA ATSUSHI 広島大学, 医歯薬保健学研究院, 研究員 (60274049) ; NISHIMOTO TAKESHI 広島大学, 医歯薬保健学研究院, 研究員 (40450580)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: pituitary / primary culture / stem cell

Outline of Final Research Achievements

The aim of our research was to reveal the way of isolation culture and the function of pituitary tumor stem cells. 60 cases non-functional pituitary adenomas were frozen and stored after the dissociation process, allowed the establishing uniform conditions for measurement of the sample. It was found that more than 70% of non- functional pituitary adenomas secreted two or more anterior pituitary hormones.
Flow cytometric analyzes of nestin, a stem cell marker, was found to be expressed in non- functional pituitary adenoma cells. Multi- secretory function of the pituitary adenoma cells has also been suggested the possibility as a stem cell. In the future, by examining the mechanism of multi- secretory function of the pituitary tumor cells, a clue to tumor stem cells might be found.

Free Research Field

neurosurgery