Multi-dimensional analyses of a novel immune system NETs-inducer using nano live imaging technology

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390419
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Niigata University (2012-2013); Osaka University (2011)
• Principal Investigator: TERAO Yutaka 新潟大学, 医歯学系, 教授 (50397717)
• Co-Investigator(Kenkyū-buntansha): KAWABATA Shigetada 大阪大学, 大学院・歯学研究科, 教授 (50273694) ; NAKATA Masanobu 大阪大学, 大学院・歯学研究科, 准教授 (90444497)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: レンサ球菌

Research Abstract

Recently, it was reported that neutrophils equips a novel immune system against pathogenic microorganisms. And then, it was named neutrophils extracellular traps, NETs. NETs work to trap the pathogenic bacteria and to present toward macrophages and other native neutrophils. However, they were still unknown what molecules derived from pathogens induces NETs, and what receptor on neutrophil recognize NETs-inducing molecule. Thus, we investigated to determine the molecules which induce NETs formation, and then how neutrophils recognize the NETs-inducing factor in this study. In addition, we examine the NETs-expressing neutrophils could eliminate major pathogenic bacteria, including Streptococcus pneumoniae, Streptococcus pyogenes, and Staphilococcus aures in vitro assys.

Research Products

• [Journal Article] Penetration kinetics of four mouthrinses into Streptococcus mutans biofilms analyzed by direct time-lapse visualization (2014)
  • Author(s): Wakamatsu, R., Takenaka, S., Ohsumi, T., Terao, Y., Ohshima, H., and Okiji, T
  • Journal Title: Clin. Oral Invest Volume: 8 Pages: 625-34
  • Peer Reviewed
• [Journal Article] STO feeder cells are useful for propagation of primarily cultured human deciduous dental pulp cells in view of elimination of contaminated bacteria and promotion of cellular outgrowth (2013)
  • Author(s): Murakami, T., Saitoh, I., Inada, E., Kurosawa, M., Iwase, Y., Noguchi, H., Terao, Y., Yamasaki, Y., Hayasaki, H., and Sato, M
  • Journal Title: Cell Medicine Volume: 6 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Streptococcus pneumoniae invades erythrocytes and utilizes them to evade human innate immunity (2013)
  • Author(s): Yamaguchi, M., Terao, Y., Mori-Yamaguchi, M., Domon, H., Sakaue, Y., Yagi, T., Nishino, K., Yamaguchi, A., Nizet, V., and Kawabata, S
  • Journal Title: PLoS one Volume: 8 Pages: e77282
  • Peer Reviewed
• [Journal Article] Cysteine proteinase from Streptococcus pyogenes enables to evade innate immunity via degradation of complement factors (2013)
  • Author(s): Honda-Ogawa, M., Ogawa, T., Terao, Y., Sumitomo, T., Nakata, M., Ikebe, K., Maeda, Y., and Kawabata, S
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 15854-64
  • Peer Reviewed
• [Journal Article] Group A streptococcal cysteine protease cleaves epithelial junctions and contributes to bacterial translocation (2013)
  • Author(s): Sumitomo, T., Nakata, M., Higashino, M., Terao, Y., and Kawabata, S
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 13317-24
  • Peer Reviewed
• [Journal Article] The virulence factors and pathogenic mechanisms on Streptococcus pyogenes (2013)
  • Author(s): Yamaguchi, M., Terao, Y., and Kawabata, S
  • Journal Title: Cell. Microbiol Volume: 15 Pages: 503-511
  • Peer Reviewed
• [Journal Article] Hydrogen peroxide produced by oral streptococci induces macrophage cell death (2013)
  • Author(s): Okahashi, N., Nakata, M., Sumitomo, T., Terao, Y., and Kawabata, S
  • Journal Title: PLoS one Volume: 8 Pages: e62563
  • Peer Reviewed
• [Journal Article] MicroRNA fragments derived from Streptococcus pyogenes enable activation of neutrophil phagocytosis : in vitro study (2013)
  • Author(s): Ogawa T., Terao Y., Honda-Ogawa M., Hashimoto S., Ikebe K., Maeda Y., and Kawabata S
  • Journal Title: Microb. Infect Volume: 15 Pages: 212-218
  • Peer Reviewed
• [Journal Article] Group A streptococcus adheres to pharyngeal epithelial cells with salivary proline-rich proteins via GrpE chaperone protein (2012)
  • Author(s): Murakami, J., Terao, Y., Morisaki, I., Hamada, S., and Kawabata, S
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 22266-22275
  • Peer Reviewed
• [Journal Article] α-enolase of Streptococcus pneumoniae induces formation of neutrophil extracellular traps (2012)
  • Author(s): Mori, Y., Yamaguchi, M., Terao, Y., Hamada, S., Ooshima, T., and Kawabata, S
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 10472-10481
  • Peer Reviewed
• [Journal Article] Long-term survival of salivary streptococci on dental devices made of EVA (2012)
  • Author(s): Ogawa, T., Yamasaki, S., Honda, M., Terao, Y., Kawabata, S., and Maeda, Y
  • Journal Title: Int. J. Oral Science Volume: 4 Pages: 14-8
  • Peer Reviewed
• [Journal Article] Involvement of T6 pili in biofilm formation by serotype M6 Streptococcus pyogenes (2012)
  • Author(s): Kimura, K. R., Nakata, M., Sumitomo, T., Kreikemeyer, B., Podbielski, A., Terao, Y., and Kawabata, S
  • Journal Title: J. Bacteriol Volume: 194 Pages: 804-812
  • Peer Reviewed
• [Journal Article] S-carboxymethylcysteine inhibits adherence of Streptococcus pneumoniae to human alveolar epithelial cells (2012)
  • Author(s): Sumitomo, T., Nakata, M., Yamaguchi, M., Terao, Y., and Kawabata S
  • Journal Title: J. Med. Microbiol Volume: 61 Pages: 101-108
  • Peer Reviewed
• [Journal Article] Assembly mechanism of FCT type 1 pili in serotype M6 Streptococcus pyogenes (2011)
  • Author(s): Nakata, M., Kimura, K. R., Sumitomo, T., Wada, S., Sugauchi, A., Oki, E., Higashino, M., Kreikemeyer, B., Podbielski, A., Okahashi, N., Hamada, S., Isoda, R., Terao, Y., and Kawabata, S
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 37566-37577
  • Peer Reviewed
• [Journal Article] Streptococcus sanguinis induces foam cell formation and cell death of macrophages in association with production of reactive oxygen species (2011)
  • Author(s): Okahashi, N., Okinaga T., Sakurai, A., Terao Y., Nakata M. Nakashima K., Shintani S., Kawabata S., Ooshima T., and Nishihara T
  • Journal Title: FEMS Microbiol. Lett Volume: 323 Pages: 164-170
  • Peer Reviewed
• [Journal Article] Novel epitopic region of glucosyltransferase B from Streptococcus mutans (2011)
  • Author(s): Hoshino, T., Kondo, Y., Saito, K., Terao, Y., Okahashi, N., Kawabata, S., and Fujiwara, T
  • Journal Title: Clin. Vaccine Immunol Volume: 18 Pages: 1552-1561
  • Peer Reviewed
• [Journal Article] Mucosal immune features to phosphorylcholine by nasal Flt3 ligand cDNA-based vaccination (2011)
  • Author(s): Baatarjav, T, Kataoka, K, Gilbert, R. S, Terao, Y, Fukui, M, Goto, M, Kawabata, S, Yamamoto, M, Fujihashi, K, and Ito, HO
  • Journal Title: Vaccine Volume: 79 Pages: 2819-2828
  • Peer Reviewed
• [Journal Article] Biofilm formation or internalization into epithelial cells enable Streptococcus pyogenes to evade antibiotic eradication in patients with pharyngitis (2011)
  • Author(s): Ogawa, T., Terao, Y., Okuni, H., Ninomiya, K., Sakata, H., Ikebe, K., Maeda, Y., and Kawabata, S
  • Journal Title: Microb. Pathog Volume: 51 Pages: 58-68
  • Peer Reviewed
• [Journal Article] Epidemiological characterization of Streptococcus pyogenes isolated from patients with multiple onsets of pharyngitis (2011)
  • Author(s): Ogawa, T., Terao, Y., Sakata, H., Okuni, H., Ninomiya, K., Ikebe, K., Maeda, Y., and Kawabata, S
  • Journal Title: FEMS Microbiol. Lett Volume: 318 Pages: 143-151
  • Peer Reviewed
• [Journal Article] Oral-nasopharyngeal dendritic cells mediate T cell-independent IgA class switching on B-1 B cells (2011)
  • Author(s): Kataoka, K., Fujihashi, K., Terao, Y., Gilbert, R. S., Sekine, S., Kobayashi, R., Fukuyama, Y., Kawabata, S., and Fujihashi, K
  • Journal Title: PLoS one Volume: 6 Pages: e25396
  • Peer Reviewed
• [Journal Article] Pili of oral Streptococcus sanguinis bind to salivary amylase and promote the biofilm formation (2011)
  • Author(s): Okahashi, N., Nakata, M., Sakurai, A., Terao, Y., Hoshino, T., Yamaguchi, Y., Isoda, R., Sumitomo, T., Nakano, K., Kawabata, S., and Ooshima, T
  • Journal Title: Microb. Pathog Volume: 50 Pages: 148-154
  • Peer Reviewed
• [Journal Article] Streptolysin S contributes to group A streptococcal translocation across an epithelial barrier (2011)
  • Author(s): Sumitomo, T., Nakata, M., Higashino, M., Jin Y., Terao, Y., Fujinaga, Y., and Kawabata, S
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 2750-2761
  • Peer Reviewed
• [Journal Article] Neonatal pleural empyema caused by emm type 6 group A streptococcus
  • Author(s): Nohara, F., Nagaya, K., Asai, H., Tsuchida, E., Okamoto, T., Hayashi, T., Sakata, H., Terao, Y., and Azuma, H
  • Journal Title: Pediatirics International Volume: (in press)
  • Peer Reviewed
• [Presentation] Lion Award 受賞講演 (2013)
  • Author(s): 寺尾 豊
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山市
  • Year and Date: 20130920-22
• [Presentation] Moleculara mechanisms of complement evasion by Streptococcus pyogenes cysteine protease SpeB (2013)
  • Author(s): 本多 真理子, 寺尾 豊, 住友 倫子, 中田匡宣, 川端 重忠
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 20130318-20
• [Presentation] Group A streptococcal cysteine proteases, SpeB and Sib35, cleave epithelial junctions (2013)
  • Author(s): Tomoko Sumitomo, Miharu Higashino, Akari Kominami, Masanobu Nakata, Yutaka Terao, and Shigetada Kawabata
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 20130318-20
• [Presentation] Cell wall anchoring mechanism of FCT region type 3 pili in Streptococcus pyogenes (2013)
  • Author(s): Masanobu Nakata, Tomoko Sumitomo, Yutaka Terao, Shigeyuki Hamada, and Shigetada Kawabata
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 20130318-20
• [Presentation] Streptococcus sanguinis induces foam cell formation and cell death of macrophages (2013)
  • Author(s): N. Okahashi, T. Okinaga, A. Sakurai, Y. Terao, M. Nakata, S. Kawabata, T. Nishihara
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 20130318-20
• [Presentation] バイオイメージング研究から始まる感染制御 (2013)
  • Author(s): 寺尾 豊
  • Organizer: 金沢医科大学大学院医学研究セミナー講演
  • Place of Presentation: 石川市
  • Year and Date: 2013-05-22
• [Presentation] ヒト補体免疫系回避機構におけるStreptococcus pyogenes 分泌型プロテアーゼSpeB の機能解析 (2013)
  • Author(s): 本多 真理子, 寺尾 豊, 住友 倫子, 中田匡宣, 川端 重忠
  • Organizer: 第5回口腔環境制御」カテゴリー集会
  • Place of Presentation: 長崎市
  • Year and Date: 2013-02-01
• [Presentation] Streptococcus pyogenesのシステインプロテアーゼSpeBが補体免疫機構に及ぼす影響 (2012)
  • Author(s): 本多 真理子, 寺尾 豊, 川端 重忠
  • Organizer: 第65回日本細菌学会関西支部総会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-11-17
• [Presentation] 細菌の感染制御に向けた次世代の基礎的研究展開 (2012)
  • Author(s): 寺尾 豊
  • Organizer: 「第60回日本化学療法西日本支部総会」「第55回日本感染症学会中日本地方会学術集会」「第82回日本感染症学会西日本地方会学術集会」三学会合同集会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-11-06
• [Presentation] Streptococcus pyogenesのシステインプロテアーゼSpeBによる補体免疫回避機構の解析 (2012)
  • Author(s): 本多 真理子, 橋本 栄, 寺尾 豊, 川端重忠
  • Organizer: 第21回Lancefieldレンサ球菌研究会
  • Place of Presentation: 吹田市
  • Year and Date: 2012-06-08
• [Presentation] T6線毛のバイオフィルム形成と菌体凝集への関与 (2012)
  • Author(s): 森田 知里, 中田 匡宣, 木村 敬次Richard, 住友 倫子, 寺尾 豊, 川端 重忠
  • Organizer: 第21回Lancefieldレンサ球菌研究会
  • Place of Presentation: 吹田市
  • Year and Date: 2012-06-08
• [Book] ナノスケールの研究を歯科医学に, 日本歯科評論 (2014)
  • Author(s): 寺尾 豊
  • Total Pages: 74(9-11)
  • Publisher: ヒョーロン・パブリッシャーズ社
• [Book] レンサ球菌感染症の予防・治療法の確立に向けた展望, 化学療法の領域 (2013)
  • Author(s): 寺尾 豊
  • Total Pages: 29(1447-1453)
  • Publisher: 医薬ジャーナル社
• [Remarks]
  • URL: http://www.dent.niigata-u.ac.jp/microbio/microbio.html