A study of bioactive materials used for filling bone defects

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390450
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tohoku University
• Principal Investigator: SUZUKI Osamu 東北大学, 歯学研究科(研究院), 教授 (60374948)
• Co-Investigator(Kenkyū-buntansha): ITOI Eiji 東北大学, 大学院・医学系研究科, 教授 (80193465) ; KAMAKURA Shinji 東北大学, 大学院・医工学研究科, 教授 (80224640) ; ANADA Takahisa 東北大学, 大学院・歯学研究科, 准教授 (30398466)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 生体材料学 / リン酸カルシウム / 天然高分子

Research Abstract

The present study was designed to investigate the effect of calcium phosphate crystal characteristics on the bioactivity of the bone substitute materials. The spongy composite consists of synthetic octacalcium phosphate (OCP) and natural polymers, such as gelatin, was prepared. OCP crystals, ground mechanically from micrometer to nano-submicro meter scale in size, were dispersed in gelatin matrix and molded. The composite was soaked in simulated body fluid for one week or implanted in rat calvaria critical-sized defect for 8 weeks and then analyzed. X-ray diffraction of the soaked materials showed that hydroxyapatite became detected with the time. Histological analysis of the decalcified specimens of the calvaria indicated that new bone was formed together with the partial biodegradation of the materials. The results suggest that the crystal characteristics may be a factor to affect the bioactivity of the composite.

Research Products

• [Journal Article] Octacalcium phosphate (OCP)-based bone substitute materials (2013)
  • Author(s): Suzuki O
  • Journal Title: Jpn Dent Sci Rev Volume: 49 Pages: 58-71
  • DOI: 10.1016/j.jdsr.2013.01.001
  • Peer Reviewed
• [Journal Article] Comparison of bone regeneration between octacalcium phosphate (OCP)/collagen composite and β-tricalcium phosphate (β -TCP) in canine calvarial defect (2013)
  • Author(s): Tanuma Y, Matsui K, Kawai T, Matsui A, Suzuki O, Kamakura S, Echigo S
  • Journal Title: Oral Sur Oral Med Oral Pathol Oral Radiol Endodontol Volume: 115 Pages: 9-17
  • DOI: 10.1016/j.oooo.2011.12.029
  • Peer Reviewed
• [Journal Article] Stimulatory capacity of an octacalcium phosphate/gelatin composite on bone regeneration in rabbit tibia defect model (2012)
  • Author(s): Suzuki K, Honda Y, Anada T, Handa T, Miyatake N, Takahashi A, Hosaka M, Imaizumi H, Itoi E, Suzuki O
  • Journal Title: Phosphorus Res Bull Volume: 26 Pages: 53-58
  • DOI: 10.3363/prb.26.53
  • Peer Reviewed
• [Journal Article] Octacalcium phosphate (OCP) collagen composites with titanium mesh facilitate alveolar augmentation in canine mandibular bone defects (2012)
  • Author(s): Miura K, Matsui K, Kawai T, Kato Y, Matsui A, Suzuki O, Kamakura S, Echigo S
  • Journal Title: Int J Oral Maxillofac Surg Volume: 41 Pages: 1161-1169
  • DOI: 10.1016/j.ijom.2012.05.020
  • Peer Reviewed
• [Journal Article] Characterization and bioactivity of nano-submicro octacalcium phosphate/gelatin composite (2011)
  • Author(s): Miura K, Anada T, Honda Y, Shiwaku Y, Kawai T, Echigo S, Takahashi T, Suzuki O
  • Journal Title: Appl Surf Sci Volume: 282 Pages: 138-145
  • DOI: 10.1016/j.apsusc.2013.05.086
  • Peer Reviewed
• [Journal Article] Evaluation of osteoclastic resorption activity using calcium phosphate coating combined with labeled polyanion (2011)
  • Author(s): Miyazaki T, Miyauchi S, Anada T, Imaizumi H, Suzuki O
  • Journal Title: Anal Biochem Volume: 410 Pages: 7-12
  • DOI: 10.1016/j.ab.2010.11.014
  • Peer Reviewed
• [Journal Article] Reconstruction of critical-sized bone defect in dog skull by octacalcium phosphate combined with collagen (2011)
  • Author(s): Kawai T, Matsui K, Iibuchi S, Anada T, Honda Y, Sasaki K, Kamakura S, Suzuki O, Echigo S
  • Journal Title: Clin Implant Dent Relate Res Volume: 13 Pages: 112-123
  • DOI: 10.1111/j.1708-8208.2009.00192.x
  • Peer Reviewed
• [Presentation] 合成リン酸オクタカルシウムの骨伝導能発現と結晶粒子特性 (2014)
  • Author(s): 鈴木治, 穴田貴久
  • Organizer: 日本機械学会第26回バイオエンジニアリング講演会
  • Place of Presentation: 仙台
  • Year and Date: 20140111-12
• [Presentation] ウサギ脛骨骨欠損へ埋入したリン酸オクタカルシウム/ゼラチン複合体の操作性と骨伝導能の検討 (2013)
  • Author(s): 鈴木堅太郎, 穴田貴久, 半田拓人, 千葉晋平, 今泉秀樹, 宮武尚央, 保坂正美, 井樋栄二, 鈴木治
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20131017-18
• [Presentation] リン酸オクタカルシウム・ゼラチン複合体の加水分解における結晶相の変化 (2013)
  • Author(s): 江副祐史, 穴田貴久, 半田拓人, 高橋哲, 鈴木治
  • Organizer: 第61回日本歯科理工学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 20130413-14
• [Presentation] 多孔性ゼラチン担体の形態が骨再生に与える影響についての検討 (2013)
  • Author(s): 神田直典, 穴田貴久, 小林司史, 高橋哲, 鈴木治
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20130321-23
• [Presentation] Cell-activating octacalcium phosphate-based scaffold materials (2013)
  • Author(s): Suzuki O
  • Organizer: NIDCR-Tohoku University Dental Colloquium
  • Place of Presentation: Sendai
  • Year and Date: 2013-05-10
• [Presentation] Octacalcium phosphate : from materials chemistry to biomaterial application (2013)
  • Author(s): Suzuki O
  • Organizer: Forsyth Institute Research Seminar
  • Place of Presentation: Cambridge, MA USA
  • Year and Date: 2013-04-10
• [Presentation] ラット頭蓋冠骨欠損におけるゼラチン担体の生体親和性検討 (2012)
  • Author(s): 神田直典, 穴田貴久, 小林司史, 高橋哲, 鈴木治
  • Organizer: 日本バイオマテリアル学会シンポジウム2012
  • Place of Presentation: 仙台
  • Year and Date: 20121126-27
• [Presentation] Analysis of differentiation of rat mesenchymal stem cells treated in osteogenic medium with basic fibroblast growth factor (2012)
  • Author(s): Kawai T, Anada T, Masuda T, Honda Y, Sakai Y, Kato Y, Echigo S, Suzuki O
  • Organizer: 9th World Biomaterials Congress
  • Place of Presentation: Chengdu, China
  • Year and Date: 20120601-05
• [Presentation] The architecture of regenerated bone by octacalcium phosphate and collagen composites (OCP/Col) in an alveolar bone defect model (2012)
  • Author(s): Matsui A, Matsui K, Handa T, Tanuma Y, Miura K, Kato Y, Kawai K, Suzuki O, Kamakura S, Echigo S
  • Place of Presentation: Chengdu, China
  • Year and Date: 20120601-05
• [Presentation] 骨再生材料ナノOCP/ゼラチン複合体の合成と機能評価 (2011)
  • Author(s): 三浦桂一郎, 穴田貴久, 本田義知, 鎌倉慎治, 越後成志, 鈴木治
  • Organizer: 第33回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 20111122-23
• [Presentation] Octacalcium phosphate (OCP) nano-particle dispersed gelatin composite as a bone substitute material (2011)
  • Author(s): Miura K, Anada T, Honda Y, Echigo S, Suzuki O
  • Organizer: Octacalcium phosphate (OCP) nano-particle dispersed gelatin composite as a bone substitute material
  • Place of Presentation: Miyagi-Zao (Togatta), Miyagi, Japan
  • Year and Date: 2011-08-08
• [Book] リン酸八カルシウム(OCP)を用いる次世代バイオマテリアルの開発. アンチ・エージングシリーズ3・骨研究最前線 (2013)
  • Author(s): 鈴木治, 穴田貴久
  • Total Pages: 325-333
  • Publisher: エヌ・ティー・エス
• [Book] Interface Oral Health Science 2011. Edits : Sasaki K, Suzuki O, Takahashi N. Synthesis of octacalcium phosphate with fluoride ions and protein adsorption onto the crystals (2012)
  • Author(s): Shiwaku Y, Anada T, Honda Y, Morimoto S, Sasaki K, Suzuki O
  • Total Pages: 237-239
  • Publisher: Springer
• [Remarks]
  • URL: http://www.cfe.dent.tohoku.ac.jp
• [Patent(Industrial Property Rights)] 骨再生材料 (2011)
  • Inventor(s): 鈴木治, 穴田貴久, 本田義知, 三浦桂一郎, 森元慎二
  • Industrial Property Rights Holder: 東北大学・ニプロ(株)
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2011-2517894
  • Filing Date: 2011-11-17