Examination of hybrid bone grafts using nano DDS and artificial bones

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390459
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: MORI Yoshiyuki 東京大学, 医学部附属病院, 准教授 (70251296)
• Co-Investigator(Kenkyū-buntansha): SAIJO Hideto 東京大学, 医学部附属病院, 講師 (80372390) ; TEI Yuichi 東京大学, 工学系研究科, 教授 (30345053) ; YANO Fumiko 東京大学, 医学系研究科, 特任助教 (80529040)
• Co-Investigator(Renkei-kenkyūsha): KATAOKA Kazunori 東京大学, 工学系研究科, 教授 (00130245) ; OGASAWARA Toru 東京大学, 医学部附属病院, 講師 (20359623)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 骨再生用移植片

Research Abstract

By further integrating research that we had performed, this study was aimed to develop hybrid-type bone grafts for bone regeneration that were efficiently replaced into bone tissues. We identified osteogenic genes and bioactive factors with the bio-sensor and developed the osteogenic differentiation protocol using the nano drug delivery of the genes and factors. Focusing on iPS cells as a cell source for bone regeneration, we attempted to establish an in vitro differentiation protocol from the cells. Finally, we generated hybrid-type bone grafts that consisted of artificial bones and osteoblastic cells generated from stem cells, and examined their bone healing ability in animal experiments.

Research Products

• [Journal Article] Implantation of tetrapod-shaped granular artificial bones or β-tricalcium phosphate granules in a canine large bone-defect model (2014)
  • Author(s): Choi S, Liu I, Yamamoto K, Honnami M, Sakai T, Ohba S, Echigo R, Suzuki S, Chung UI, Sasaki N, Mochizuki M
  • Journal Title: J Vet Med Sci Volume: 76(2) Pages: 229-235
• [Journal Article] Repair of rabbit segmental femoral defects by using a combination of tetrapod-shaped granular artificial bones and basic fibroblast growth factor-binding ion complex gel (2013)
  • Author(s): Honnami M, Choi S, Liu I, Kamimura W, Taguchi T, Hojo H, Shimohata N, Ohba S, Koyama H, Nishimura R, Chung UI, Sasaki N, Mochizuki M
  • Journal Title: Biomaterials Volume: 34(36) Pages: 9056-9062
• [Journal Article] Generation of Col2a1-EGFP iPS cells for monitoring chondrogenic differentiation (2013)
  • Author(s): Saito T, Yano F, Mori D, Ohba S, Hojo H, Otsu M, Eto K, Nakauchi H, Tanaka S, Chung UI, Kawaguchi H
  • Journal Title: PLoS ONE Volume: 8(9) Pages: e74137
• [Journal Article] Nanog promotes osteogenic differentiation of the mouse mesenchymal cell line C3H10T1/2 by modulating bone morphogenetic protein (BMP) signaling (2013)
  • Author(s): Ogasawara T, Ohba S, Yano F, Kawaguchi H, Chung UI, Saito T, Yonehara Y, Nakatsuka T, Mori Y, Takato T, Hoshi K
  • Journal Title: J Cell Physiol Volume: 228(1) Pages: 163-171
• [Journal Article] Submucous cleft palate : variations in bony defects of the hard palate (2013)
  • Author(s): Mori Y, Hoshi K, Takato T, Takahashi M, Hirano Y, Kanno Y, Ohkubo K, Saijo H
  • Journal Title: Br J Oral Maxillofac Surg Volume: 51 Pages: e220-223
• [Journal Article] Usefulness of agarose mold as a storage container for three-dimensional tissue-engineered cartilage (2013)
  • Author(s): Mori Y, Kanazawa S, Watanabe M, Suenaga H, Nagata S, Fujihara Y, Takato T, Hoshi K
  • Journal Title: Mater Sci Appl Volume: 4(8) Pages: 73
• [Journal Article] Hollow fiber module applied for effective proliferation and harvest of cultured chondrocytes (2013)
  • Author(s): Mori Y, Watanabe M, Nakagawa S, Asawa Y, Nishizawa S, Saijo H, Nagata S, Fujihara Y, Takato T, Hoshi K
  • Journal Title: Mater Sci Appl Volume: 4(8) Pages: 62-7
• [Journal Article] Clinical presentation of epignathus teratoma with cleft palate ; and duplication of cranial base, tongue, mandible, and pituitary gland (2013)
  • Author(s): Maeda Y, Suenaga H, Sugiyama M, Saijo H, Hoshi K, Mori Y, Takato T
  • Journal Title: J Craniofac Surg Volume: 24(4) Pages: 1486-91
• [Journal Article] Development and evaluation of tetrapod-shaped granular artificial bones (2012)
  • Author(s): Choi S, Liu IL, Yamamoto K, Igawa K, Mochizuki M, Sakai T, Echigo R, Honnami M, Suzuki S, Chung UI, Sasaki N
  • Journal Title: Acta Biomater Volume: 8(6) Pages: 2340-7
• [Journal Article] Mandibular body ostectomy for correction of mandibular prognathism - a technical note (2012)
  • Author(s): Mori Y, Susami T, Saijo H, Okubo K, Uchino N, Hoshi K, Takato T
  • Journal Title: Oral Sci Int Volume: 9(1) Pages: 21-25
• [Journal Article] The efficacy of dental therapy for an adult case of Henoch-Schönlein Purpura (2012)
  • Author(s): Abe M, Mori Y, Saijo H, Hoshi K, Ohkubo K, Ono T, Takato T
  • Journal Title: Oral Sci Int Volume: 9(2) Pages: 59-62
• [Journal Article] Extraction of 6 molars under general anesthesia in patient with fibrodysplasia ossificans progressiva (2011)
  • Author(s): Mori Y, Susami T, Haga N, Tamura K, Kanno Y, Saijo H, Takato T
  • Journal Title: J Oral Maxillofac Surg Volume: 69(7) Pages: 1905-10
• [Journal Article] Bone regeneration by the combined use of tetrapod-shaped calcium phosphate granules with basic fibroblast growth factor-binding ion complex gel in canine segmental radial defects
  • Author(s): Honnami M, Choi S, Liu IL, Kamimura W, Taguchi T, Hojo H, Shimohata N, Ohba S, Koyama H, Nishimura R, Chung UI, Sasaki N, Mochizuki M
  • Journal Title: J Vet Med Sci Volume: (in press)
• [Presentation] Stepwise differentiation of pluripotent stem cells into osteoblasts using four small molecules under serum-free and feeder-free conditions (2013)
  • Author(s): Kanke K, Saito S, Hojo H, Lichtler AC, Chung UI, Ohba S
  • Organizer: Cell symposia 2013- Stem cells to model and treat human disease
  • Place of Presentation: Los Angeles, CA, USA
  • Year and Date: 20131100
• [Presentation] Stepwise differentiation of pluripotent stem cells into osteoblasts with four small molecules under a serum-free condition (2013)
  • Author(s): Kanke K, Saito T, Hojo H, Lichtler AC, Chung UI, Ohba S
  • Organizer: 35th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Baltimore, MD, USA
  • Year and Date: 20131000
• [Presentation] in situ Tissue Engineering 型カスタムメイド人工骨の骨癒合状態のCT による評価 (2013)
  • Author(s): 菅野勇樹, 西條英人 , 鄭雄一, 森良之, 高戸毅
  • Organizer: 第58回日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡
  • Year and Date: 20131000
• [Presentation] Outcome of the surgical-orthodontic treatment in patients with clefts using the two-stage surgery combining maxillary distraction with mandibular setback osteotomies (2013)
  • Author(s): Mori Y, Susami T, Saijo H, Uchino N, Ohkubo K, Inokuchi T, Okayasu M, Uwatoko K, Hoshi K, Takato T
  • Organizer: 12th International Congress on Cleft Lip/Palate and Related Craniofacial Anomalies
  • Place of Presentation: Florida USA , Hilton Orlando Lake Buena Vista
  • Year and Date: 20130505-10
• [Presentation] Novel approach for the differentiation of embryonic stem cells into osteoblasts under a chemically-defined condition (2013)
  • Author(s): Kanke K, Hojo H, Lichtler AC, Chung UI, Ohba S
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20130500
• [Presentation] 下顎枝矢状分割術における超音波骨切削器(ピエゾサージェリーシステム[〇!R])を用いたShort lingual osteotomy (SLO) (2012)
  • Author(s): 森良之, 末永英之, 古賀陽子, 菅野勇樹, 杉山円, 瀬戸一郎, 西條英人, 星和人, 高戸毅
  • Organizer: 第57回(公社)日本口腔外科学会総会・学術集会
  • Place of Presentation: パシフィコ横浜会議センター, 神奈川県横浜市
  • Year and Date: 20121019-21
• [Presentation] Gli1 Participates in the Indian Hedgehog-mediated Osteogenesis during Endochondral Ossification (2012)
  • Author(s): Hojo H, Ohba S, Yano F, Saito T, Ikeda T, Nakajima K, Komiyama Y, Nakagata H, Suzuki K, Takato T, Kawaguchi H, Chung UI
  • Organizer: 34th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Minneapolis, MN, USA
  • Year and Date: 20121000
• [Presentation] Osteo-chondrogenic Function of BMP is Directed Toward Osteogenesis by Hh-Gli1 in the Perichondrium (2012)
  • Author(s): Hojo H, Ohba S, Taniguchi K, Shirai M, Yano F, Saito T, Ikeda T, Nakajima K, Komiyama Y, Nakagata N, Suzuki K, Mishina Y, Yamada M, Konno T, Takato T, Kawaguchi H, Kambara H, Chung UI
  • Organizer: 34th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Minneapolis, MN, USA
  • Year and Date: 20121000
• [Presentation] Prevention and Repair of Cartilage Degeneration by a Novel Small Thienoindazole-derivative Compound (2012)
  • Author(s): Yano F, Hosaka Y, Fukai A, Saito T, Hojo H, Ohba S, Kawaguchi H, Chung UI
  • Organizer: 2012 World Congress on Osteoarthritis
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20120400
• [Presentation] A 2-stage surgery combining mandibular distraction with a Le Fort I maxillary osteotomy in a patient with hemifacial microsomia (2011)
  • Author(s): Mori Y, Susami T, Saijo H, Ohkubo K, Kanno Y, Suenaga H, Seto I, Hoshi K, Takato T
  • Organizer: 9th European Craniofacial Congress
  • Place of Presentation: Salzburg, Austria, Salzburg Congress
  • Year and Date: 20110914-17
• [Presentation] 口腔前癌病変悪性化におけるエピジェネティクスを指標としたリスクマーカーのゲノム網羅的探索 (2011)
  • Author(s): 阿部雅修, 森良之, 西條英人, 安部貴大, 杉山円, 古賀陽子, 高戸毅
  • Organizer: 第35回日本頭頸部癌学会
  • Place of Presentation: ウィンクあいち, 名古屋
  • Year and Date: 20110608-10
• [Book] 「15章消化器疾患 5口腔疾患」内科学, 門脇孝・永井良三総編集 (2012)
  • Author(s): 森良之・高戸毅
  • Total Pages: 838-841
  • Publisher: 西村書店(東京)
• [Remarks] 東京大学医学部附属病院顎口腔外科・歯科矯正歯科ホームページ
  • URL: http://plaza.umin.ac.jp/~oralsurg/