2013 Fiscal Year Final Research Report
Developing of differentiation inducing therapy on oral cancer by targeting transcription factor Brachyury which regulates cancer stem cells
| Project/Area Number |
23390465
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
SUGIURA Tsuyoshi 九州大学, 歯学研究科(研究院), 准教授 (40322292)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | Brachyury / 癌幹細胞 |
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| Research Abstract |
We reported that the T-box transcription factor Brachyury is a potential regulator of cancer stem cells (CSCs). Specifically, growth of CSCs was controlled by Brachyury knockdown in AdCC. Since CSCs are resistant to chemotherapy and radiotherapy, this finding provides a new principle for therapies targeting CSCs. In the present study, we established that Brachyury knockdown suppresses chemo- and radioresistance in vitro. Brachyury was knocked down by transfecting Brachyury short hairpin RNA (shRNA) into the AdCC CSC cell line, ACCS-M GFP. Brachyury knockdown significantly inhibited cell migration and invasion and suppressed chemoresistance. Furthermore, ACCS-M GFP radioresistance was significantly suppressed by Brachyury knockdown. Brachyury knockdown significantly sensitized ACCS-M GFP cells to chemoradiotherapy. This study demonstrates that Brachyury knockdown reduces invasiveness and chemo- and radioresistance of CSCs in vivo.
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[Journal Article] CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells2012
Author(s)
Chigita, S., Sugiura, T ., Abe, M., Kobayashi, Y., Shimoda, M., Onoda, M. & Shirasuna, K
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Journal Title
International journal of oncology
Volume: 41
Pages: 2021-2028
DOI
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[Journal Article] T-box transcription factor Brachyury expression is correlated with epithelial-mesenchymal transition and lymph node metastasis in oral squamous cell carcinoma2012
Author(s)
Imajyo, I., Sugiura, T., Kobayashi, Y., Shimoda, M., Ishii, K., Akimoto, N., Yoshihama, N., Kobayashi, I. & Mori, Y
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Journal Title
International journal of oncology
Volume: 41
Pages: 1985-1995
DOI
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[Journal Article] The T-box transcription factor Brachyury regulates epithelial-mesenchymal transition in association with cancer stem-like cells in adenoid cystic carcinoma cells2012
Author(s)
Shimoda, M., Sugiura, T., Imajyo, I., Ishii, K., Chigita, S., Seki, K., Kobayashi, Y. & Shirasuna, K
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Journal Title
BMC cancer
Volume: 12
Pages: 377
DOI
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[Journal Article] Involvement of epithelial-mesenchymal transition in adenoid cystic carcinoma metastasis2011
Author(s)
Ishii, K., Shimoda, M., Sugiura, T., Seki, K., Takahashi, M., Abe, M., Matsuki, R., Inoue, Y. & Shirasuna, K
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Journal Title
International journal of oncology
Volume: 38
Pages: 921-931
DOI
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