Investigation of mechanism of systemic diseases caused by oral bacteria and development of preventive procedures

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390472
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Osaka University
• Principal Investigator: OOSHIMA Takashi 大阪大学, 歯学研究科(研究院), 招へい教員 (80116003)
• Co-Investigator(Kenkyū-buntansha): NAKANO Kazuhiko 大阪大学, 歯学研究科, 准教授 (00379083) ; NOMURA Ryota 大阪大学, 歯学部附属病院, 講師 (90437385) ; NEMOTO Hirotoshi 大阪大学, 歯学部附属病院, 医員 (80527226)
• Research Collaborator: UMEMURA Kazuo 浜松医科大学, 医学部, 教授 (40232912) ; TANAKA Tokutaro 聖隷浜松病院, 脳神経外科, 部長 (90283366) ; HOKAMURA Kazuya 浜松医科大学, 医学部, 助教 (90436965) ; NAKA Shuhei 大阪大学, 歯学部附属病院, 医員 (10589774) ; URATA Ayuchi 大阪大学, 歯学研究科, 特任研究員 (90589772)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 口腔細菌 / 全身疾患 / ミュータンスレンサ球菌 / う蝕 / 感染性心内膜炎 / 脳出血 / 炎症性腸炎 / 非アルコール性脂肪肝炎

Research Abstract

Recently, the association of periodontitis with several systemic diseases has received attention. In the present study, the contribution of Streptococcus mutans, a major pathogen of dental caries, to the pathogenesis of infective endocarditis, cerebral hemorrhage, inflammatory bowel disease, and non-alcoholic steatohepatitis was investigated by focusing on cell surface collagen-binding proteins. Our results showed that S. mutans with collagen-binding proteins is possibly virulent for these diseases following invsion of the bloodstream.

Research Products

• [Journal Article] Potential involvement of collagen-binding proteins of Streptococcus mutans in infective endocarditis (2013)
  • Author(s): Nomura R, Naka S, Nemoto H, Inagaki S, Taniguchi K, Ooshima T, Nakano K
  • Journal Title: Oral Dis Volume: 19 Pages: 387-393
• [Journal Article] Multilocus sequence typing of Streptococcus mutans with cbm gene encoding a novel collagen-binding protein (2013)
  • Author(s): Lapirattanakul J, Nomura R, Nemoto H, Naka S, Ooshima T, Nakano K
  • Journal Title: Arch Oral Biol Volume: 58 Pages: 989-996
• [Journal Article] Potential high virulence for infective endocarditis in Streptococcus mutans strains with collagen-binding proteins but lacking PA expression (2013)
  • Author(s): Nomura R, Naka S, Nemoto H, Otsugu M, Nakamura S, Ooshima T, Nakano K
  • Journal Title: Arch Oral Biol Volume: 58 Pages: 1627-1634
• [Journal Article] Distribution of amoxicillin-resistant oral streptococci in dental plaque specimens obtained from Japanese children and adolescents at risk for infective endocarditis (2013)
  • Author(s): Nemoto H, Nomura R, Ooshima T, Nakano K
  • Journal Title: J Cardiol Volume: 62 Pages: 296-300
• [Journal Article] Aggravation of inflammatory bowel diseases by oral streptococci (2013)
  • Author(s): Kojima A, Nomura R, Naka S, Okawa R, Ooshima T, Nakano K
  • Journal Title: Oral Dis Volume: (in press)
• [Journal Article] A specific Streptococcus mutans strain aggravates non-alcoholic fatty liver disease (2013)
  • Author(s): Naka S, Nomura R, Takashima Y, Okawa R, Ooshima T, Nakano K
  • Journal Title: Oral Dis Volume: (in press)
• [Journal Article] Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease (2012)
  • Author(s): Yoneda M, Naka S, Nakano K, Wada K, Endo H, Mawatari H, Imajo K, Nomura R, Hokamura K, Ono M, Murata S, Tohnai I, Sumida Y, Shima T, Kuboniwa M, Umemura K, Kamisaki Y, Amano A, Okanoue T, Ooshima T, Nakajima A
  • Journal Title: BMC Gastroenterol Volume: 12 Pages: 16
• [Journal Article] Infection of specific strains of Streptococcus mutans, oral bacteria, confers a risk of ulcerative colitis (2012)
  • Author(s): Kojima A, Nakano K, Wada K, Takahashi H, Katayama K, Yoneda M, Higurashi T, Nomura R, Hokamura K, Muranaka Y, Matsuhashi N, Umemura K, Kamisaki Y, Nakajima A, Ooshima T
  • Journal Title: Sci Rep Volume: 2 Pages: 232
• [Journal Article] Amoxicillin-resistant oral streptococci identified in dental plaque specimens from healthy Japanese adults (2012)
  • Author(s): Masuda K, Nemoto H, Nakano K, Naka S, Nomura R, Ooshima T
  • Journal Title: J Cardiol Volume: 59 Pages: 285-290
• [Journal Article] Identification and characterization of a collagen-binding protein, Cbm, in Streptococcus mutans (2012)
  • Author(s): Nomura R, Nakano K, Naka S, Nemoto H, Masuda K, Lapirattanakul J, Alaluusua S, Matsumoto M, Kawabata S, Ooshima T
  • Journal Title: Mol Oral Microbiol Volume: 27 Pages: 308-323
• [Journal Article] Common knowledge regarding prevention of infective endocarditis among general dentists in Japan (2011)
  • Author(s): Nakano K, Ooshima T
  • Journal Title: J Cardiol Volume: 57 Pages: 123-130
• [Journal Article] Characterization of aortic aneurysm in cardiovascular disease patients harboring Porphyromonas gingivalis (2011)
  • Author(s): Nakano K, Wada K, Nomura R, Nemoto H, Inaba H, Kojima A, Naka S, Hokamura K, Mukai T, Hata H, Toda K, Nakajima A, Umemura K, Kamisaki Y, Yoshioka H, Taniguchi K, Amano A, Ooshima T
  • Journal Title: Oral Dis Volume: 17 Pages: 370-378
• [Journal Article] Isolation and characterization of Streptococcus mitis from blood of child with osteomyelitis (2011)
  • Author(s): Nomura R, Nakano K, Mäkelä K, Vaara M, Salo E, Alaluusua S, Ooshima T
  • Journal Title: Int J Paediatr Dent Volume: 21 Pages: 192-199
• [Journal Article] The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke (2011)
  • Author(s): Nakano K, Hokamura K, Taniguchi N, Wada K, Kudo C, Nomura R, Kojima A, Naka S, Muranaka Y, Thura M, Nakajima A, Masuda K, Speziale P, Shimada N, Amano A, Kamisaki Y, Tanaka T, Umemura K, Ooshima T
  • Journal Title: Nat Commun Volume: 2 Pages: 485
• [Journal Article] Multilocus sequence typing analysis of Streptococcus mutans strains with the cnm gene encoding a collagen-binding adhesin (2011)
  • Author(s): Lapirattanakul J, Nakano K, Nomura R, Leelataweewud P, Chalermsarp N, Klaophimai A, Srisatjaluk R, Hamada S, Ooshima T
  • Journal Title: J Med Microbiol Volume: 60 Pages: 1677-1684
• [Journal Article] Distribution of oral streptococci highly resistant to amoxicillin in dental plaque specimens from Japanese children and adolescents (2011)
  • Author(s): Nemoto H, Nakano K, Masuda K, Wada K, Ardin AC, Nomura R, Ooshima T
  • Journal Title: J Med Microbiol Volume: 60 Pages: 1853-1859
• [Presentation] 小児歯科研究最前線 「齲蝕原性細菌の関与する全身疾患」 (2013)
  • Author(s): 野村良太
  • Organizer: 第32回日本小児歯科学会中四国地方会大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-11-24
• [Presentation] 口腔細菌による歯科疾患と全身疾患 (2013)
  • Author(s): 仲野和彦
  • Organizer: シンポジウム3「全身性疾患の危険因子としての口腔内常在細菌」, 第54回日本人間ドック学会
  • Place of Presentation: 浜松
  • Year and Date: 2013-08-30
• [Presentation] Virulence of Streptococcus mutans strains for the development of Non-alcoholic fatty liver disease (2013)
  • Author(s): Naka S, Nomura R, Nakano K, Ooshima T
  • Organizer: 60th ORCA (European Organization of Caries Research) Congress
  • Place of Presentation: Liverpool, UK
  • Year and Date: 2013-07-03
• [Presentation] Involvement of cell surface protein expression patterns in interaction of Streptococcus mutans with vein endothelial cells (2013)
  • Author(s): Nomura R, Otsugu M, Nakano K, Ooshima T
  • Organizer: 60th ORCA (European Organization of Caries Research) Congress
  • Place of Presentation: Liverpool, UK
  • Year and Date: 2013-07-03
• [Presentation] Streptococcus mutans における新規コラーゲン結合タンパクの同定と感染性心内膜炎に対する病原性の解析 (2013)
  • Author(s): 野村良太
  • Organizer: 平成24年度日本小児歯科学会学術賞受賞講演
  • Place of Presentation: 岐阜
  • Year and Date: 2013-05-23
• [Presentation] Aggravation of inflammatory bowel diseases by Streptococcus sanguinis (2013)
  • Author(s): Kojima A, Nomura R, Ooshima T, Nakano K
  • Organizer: 91st IADR (International Association of Dental Research) Meeting
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-03-20
• [Presentation] Specific Streptococcus mutans strain aggravates non-alcoholic fatty liver disease (2013)
  • Author(s): Naka S, Nomura R, Ooshima T, Nakano K
  • Organizer: 91st IADR (International Association of Dental Research) Meeting
  • Place of Presentation: Seattle, USA
  • Year and Date: 2013-03-20
• [Presentation] Serotype classification of Streptococcus mutans and its association with systemic virulence. -Impact of Oral Health on the Systemic Health; Lessons from Human and Animal Studies - (2012)
  • Author(s): Nakano K
  • Organizer: 60th JADR (Japanese Association of Dental Research) Meeting
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2012-12-15
• [Presentation] Cell surface proteins of Streptococcus mutans contribute to infective endocarditis (2012)
  • Author(s): Nomura R, Nakano K
  • Organizer: 60th JADR (Japanese Association for Dental Research) Meeting
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2012-12-15
• [Presentation] Contribution of Protein antigen to Streptococcus mutans-induced cerebral hemorrhage deterioration (2012)
  • Author(s): Nomura R, Nakano K, Naka S, Ooshima T
  • Organizer: 90th IADR (International Association of Dental Research) Meeting
  • Place of Presentation: Iguacu falls, Brazil
  • Year and Date: 2012-06-20
• [Presentation] Porphyromonas gingivalis causes development of non-alcoholic steatohepatitis in mouse model (2012)
  • Author(s): Naka S, Nakano K, Nomura R, Ooshima T
  • Organizer: 90th IADR (International Association of Dental Research) Meeting
  • Place of Presentation: Iguacu falls, Brazil
  • Year and Date: 2012-06-20
• [Presentation] コラーゲン結合能を有するStreptococcus mutans 菌株の分布と血管内皮細胞への付着侵入能の解析 (2012)
  • Author(s): 野村良太, 仲 周平, 仲野和彦, 大嶋 隆
  • Organizer: 第22回レンサ球菌研究会
  • Place of Presentation: 吹田
  • Year and Date: 2012-06-08
• [Presentation] Streptococcus mutans の引き起こす腸炎悪化におけるIFN-γの役割に対する分析 (2012)
  • Author(s): 小島あゆち, 仲野和彦, 野村良太, 大嶋隆
  • Organizer: 第50回日本小児歯科学会記念大会
  • Place of Presentation: 東京
  • Year and Date: 2012-05-12
• [Presentation] マウス非アルコール性脂肪肝炎モデルにおけるPorphyromonas gingivalis 感染による肝臓組織での遺伝子発現 (2012)
  • Author(s): 仲 周平, 仲野和彦, 野村良太, 大嶋 隆
  • Organizer: 第50回日本小児歯科学会記念大会
  • Place of Presentation: 東京
  • Year and Date: 2012-05-12
• [Presentation] Streptococcus mutans の引き起こす脳出血増悪における190kDa Protein antigen (PA) の役割 (2012)
  • Author(s): 野村良太, 仲野和彦, 仲 周平, 大嶋 隆
  • Organizer: 第50回日本小児歯科学会記念大会
  • Place of Presentation: 東京
  • Year and Date: 2012-05-12
• [Presentation] う蝕病原細菌Streptococcus mutans の菌体表層PA タンパクの脳出血増悪因子としての役割 (2012)
  • Author(s): 仲野和彦, 外村和也, 野村良太, 仲 周平, 田中篤太郎, 梅村和夫, 大嶋 隆
  • Organizer: 第37回脳卒中学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2012-04-26
• [Presentation] 高病原性う蝕原因菌Streptococcus mutans 由来のリコンビナントタンパクによって脳出血が増悪する (2012)
  • Author(s): 外村和也, 仲野和彦, 野村良太, 大嶋 隆, 大橋寿彦, 細井泰志, 田中篤太郎, 梅村和夫
  • Organizer: 第37回脳卒中学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2012-04-26
• [Presentation] Streptococcus mutans のコラーゲン結合タンパクについて (2012)
  • Author(s): 野村良太, 仲野和彦, 仲 周平, 大嶋 隆
  • Organizer: 第113回大阪大学歯学会例会
  • Place of Presentation: 吹田
  • Year and Date: 2012-01-12
• [Presentation] 脳出血における増悪因子としてのう蝕病原細菌Streptococcus mutans (2011)
  • Author(s): 仲野和彦, 野村良太, 外村和也, 大橋寿彦, 細井泰志, 田中篤太郎, 梅村和夫, 大嶋 隆
  • Organizer: 第36回脳卒中学会総会
  • Place of Presentation: 京都
  • Year and Date: 2011-07-31
• [Presentation] Distribution of Streptococcus mutans strains with collagen-binding properties (2011)
  • Author(s): Naka S, Nakano K, Nomura R, Ooshima T
  • Organizer: 23rd meeting of IAPD (International Association of Paediatric Dentistry)
  • Place of Presentation: Athens, Greece
  • Year and Date: 2011-06-15
• [Book] Molecular typing of Streptococcus mutans. In : Molecular Typing of Bacterial Infection. De Filipis I, McKee ML editors (2013)
  • Author(s): Nakano K, Nakagawa I, Alaluusua S, Ooshima T
  • Total Pages: 127-147
  • Publisher: Springer, New York
• [Remarks]
  • URL: http://web.dent.osaka-u.ac.jp/~pedo/