2013 Fiscal Year Final Research Report
Periodontal disease susceptibility evaluated on membrane traffic machinery
| Project/Area Number |
23390477
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Periodontal dentistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
AMANO Atsuo 大阪大学, 歯学研究科(研究院), 教授 (50193024)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUBONIWA Masae 大阪大学, 歯学部附属病院, 講師 (00303983)
FURUTA Nobumichi 大阪大学, 大学院・歯学研究科, 助教 (50452446)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 歯学 / 歯周病菌 / 歯周病 / 細菌・組織 / メンブレントラフィック / 細胞内侵入 |
|---|
| Research Abstract |
Gingival epithelial cells function as innate host defense barriers to prevent intrusion by periodontal bacteria. Nevertheless, Porphyromonas gingivalis can enter these cells and pass through the epithelial barrier into deeper tissues. In this study, P. gingivalis induced cellular invagination to internalize the pathogen. The bacteria were sorted to early endosomes, and the half of them were transported to recycling pathway via SNARE protein VAMP2, which allowed the bacterial exit to the extracellular circumstances, a mechanism of cell-to-cell spread within periodontal tissues. The intracellular pathogens impaired fundamental cellular functions within the cargo. While, remaining half were sorted to lytic compartments, including autophagosomes. The machinery was found to be formed in contact sites between endoplasmic reticulum and mitochondria, which is a very unique finding indicating that 2 different organelle make a concerted effort to the formation of another organelle.
|
Research Products
(48 results)
-
-
-
-
-
-
-
[Journal Article] Autophagosome forms at the ER-mitochondria contact sites2013
Author(s)
Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Ohmori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
-
Journal Title
Nature
Volume: 495(7441)
Pages: 389-393
DOI
-
-
-
-
-
-
-
[Journal Article] Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease2012
Author(s)
Yoneda M, Naka S, Nakano K, Wada K, Endo H, Mawatari H, Imajo K, Nomura R, Hokamura K, Ono M, Murata S, Tohnai I, Sumida Y, Shima T, Kuboniwa M, Umemura K, Kamisaki Y, Amano A, Okanoue T, Ooshima T, Nakajima A
-
Journal Title
BMC Gastroenterology
Volume: 12(1)
Pages: 16
DOI
-
-
-
-
[Journal Article] Distribution and molecular characterization of Porphyromonas gulae carrying a new fimA genotype2012
Author(s)
Yamasaki Y, Nomura R, Nakano K, Inaba H, Kuboniwa M, Hirai N, Shirai M, Kato Y, Murakami M, Naka S, Iwai S, Matsumoto-Nakano M, Ooshima T, Amano A, Asai F
-
Journal Title
Veterinary Microbiology
Volume: 161(1)
Pages: 196-205
DOI
-
-
-
-
-
-
[Journal Article] The collagen-binding protein of Streptococcus mutans is involved in hemorrhagic stroke2011
Author(s)
Nakano K, Hokamura K, Taniguchi N, Wada K, Kudo C, Nomura R, Kojima A, Naka S, Muranaka Y, Thura M, Nakajima A, Masuda K, Nakagawa I, Speziale P, Shimada N, Amano A, Kamisaki Y, Tanaka T, Umemura K. Ooshima T
-
Journal Title
Nature Communications
Volume: 2
Pages: 485
DOI
-
-
-
[Journal Article] Characterization of aortic aneurysms in cardiovascular disease patients harboring Porphyromonas gingivalis2011
Author(s)
Nakano K, Wada K, Nomura R, Nemoto H, Inaba H, Kojima A, Naka S, Hokamura K, Mukai T, Nakajima A, Umemura K, Kamisaki Y, Yoshioka H, Taniguchi K, Amano A, Ooshima T
-
Journal Title
Oral Diseases
Volume: 17(4)
Pages: 370-378
DOI
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
