• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Periodontal disease susceptibility evaluated on membrane traffic machinery

Research Project

  • PDF
Project/Area Number 23390477
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka University

Principal Investigator

AMANO Atsuo  大阪大学, 歯学研究科(研究院), 教授 (50193024)

Co-Investigator(Kenkyū-buntansha) KUBONIWA Masae  大阪大学, 歯学部附属病院, 講師 (00303983)
FURUTA Nobumichi  大阪大学, 大学院・歯学研究科, 助教 (50452446)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywords歯学 / 歯周病菌 / 歯周病 / 細菌・組織 / メンブレントラフィック / 細胞内侵入
Research Abstract

Gingival epithelial cells function as innate host defense barriers to prevent intrusion by periodontal bacteria. Nevertheless, Porphyromonas gingivalis can enter these cells and pass through the epithelial barrier into deeper tissues. In this study, P. gingivalis induced cellular invagination to internalize the pathogen. The bacteria were sorted to early endosomes, and the half of them were transported to recycling pathway via SNARE protein VAMP2, which allowed the bacterial exit to the extracellular circumstances, a mechanism of cell-to-cell spread within periodontal tissues. The intracellular pathogens impaired fundamental cellular functions within the cargo. While, remaining half were sorted to lytic compartments, including autophagosomes. The machinery was found to be formed in contact sites between endoplasmic reticulum and mitochondria, which is a very unique finding indicating that 2 different organelle make a concerted effort to the formation of another organelle.

  • Research Products

    (48 results)

All 2014 2013 2012 2011 Other

All Journal Article (28 results) Presentation (13 results) (of which Invited: 13 results) Book (6 results) Remarks (1 results)

  • [Journal Article] Porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of proMMP9 and its activation2014

    • Author(s)
      Inaba H, Sugita H, Kuboniwa M, Iwai S, Hamada M, Noda T, Morisaki I, Lamont RJ, Amano A
    • Journal Title

      Cellular Microbiology

      Volume: 16(1) Pages: 131-145

    • DOI

      10.1111/cmi.12211

  • [Journal Article] Transcellular offensive weapons of Porphyromonas gingivalis in host-parasite interactions2014

    • Author(s)
      Amano A, Kuboniwa M, Takeuchi H
    • Journal Title

      Journal of Oral Bioscience

      Volume: 56(3)(in press)

  • [Journal Article] Genetic characteristics and pathogenic mechanisms of periodontal pathogens2014

    • Author(s)
      Amano A, Chen C, Honma K, Li C, Settem RP, Sharma A
    • Journal Title

      Advances in Dental Research

      Volume: 26(1) Pages: 15-22

    • DOI

      10.1177/0022034514526237

  • [Journal Article] Erythritol alters microstructures and metabolomic profiles of biofilm composed of Streptococcus gordonii and Porphyromonas gingivalis2013

    • Author(s)
      Hashino E, Kuboniwa M, Alghamdi S, Yamaguchi M, Yamamoto R, Cho H, Amano A
    • Journal Title

      Molecular Oral Microbiology

      Volume: 28(6) Pages: 435-451

    • DOI

      10.1111/omi.12037

  • [Journal Article] Expression pattern of zinc-finger transcription factor Odd-skipped related 2 in murine development and neonatal stage2013

    • Author(s)
      Kawai S, Michikami I, Kitagaki J, Hashino E, Amano A
    • Journal Title

      Gene Expression Patterns

      Volume: 13(8) Pages: 372-376

    • DOI

      10.1016/j.gep.2013.06.007

  • [Journal Article] Genotype analysis of Porphyromonas gingivalis fimA in Korean adults using new primers2013

    • Author(s)
      Moon J-H, Herr Y, Lee H-W, Shin S-I, Kim C, Amano A, Lee J-Y
    • Journal Title

      Journal of Medical Microbiology

      Volume: 62 Pages: 1290-1294

  • [Journal Article] Autophagosome forms at the ER-mitochondria contact sites2013

    • Author(s)
      Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Ohmori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
    • Journal Title

      Nature

      Volume: 495(7441) Pages: 389-393

    • DOI

      10.1038/nature11910†Equalcontributors

  • [Journal Article] The serine phosphatase SerB of Porphyromonas gingivalis suppresses IL-8 production by dephosphorylation of NF-κB RelA/p652013

    • Author(s)
      Takeuchi H, Hirano T, Whitmore SE, Morisaki I, Amano A, Lamont RJ
    • Journal Title

      PLoS Pathogens

      Volume: 9(4) Pages: e1003326

    • DOI

      10.1371/journal.ppat.1003326

  • [Journal Article] CRISPR regulation of intraspecies diversification by limiting IS transposition and intercellular recombination2013

    • Author(s)
      Watanabe T, Nozawa T, Aikawa C, Amano A, Maruyama F, Nakagawa I
    • Journal Title

      Genome Biology and Evolution

      Volume: 5(6) Pages: 1099-1114

    • DOI

      10.1016/j.gep.2013.06.007

  • [Journal Article] Identification and characterization of Porphyromonas gingivalis client proteins that bind to Streptococcus oralis Glyceraldehyde-3-Phosphate Dehydrogenase2013

    • Author(s)
      Maeda K, Nagata H, Kuboniwa M, Ojima M, Osaki T, Minamino N, Amano A
    • Journal Title

      Infection and Immunity

      Volume: 81(3) Pages: 753-763

    • DOI

      10.1128/IAI.00875-12

  • [Journal Article] Porphyromonas gingivalis fimbriae2013

    • Author(s)
      Enersen M, Nakano K, Amano A
    • Journal Title

      Journal of Oral Microbiology

      Volume: 5 Pages: 10

    • DOI

      10.3402/jom.v5i0.20265

  • [Journal Article] Transition metal ions induce carnosinase activity in PepD-homologous protein from Porphyromonas gingivalis2012

    • Author(s)
      Aoki A, Shibata Y, Okano S, Maruyama F, Amano A, Nakagawa I, Abiko Y
    • Journal Title

      Microbial Pathogenesis

      Volume: 52(1) Pages: 17-24

    • DOI

      10.1016/j.micpath.2011.09.003

  • [Journal Article] Development and evaluation of new primers for PCR-based identification of type II fimA of Porphyromonas gingivalis2012

    • Author(s)
      Moon JH, Shin SI, Chung JH, Lee SW, Amano A, Lee JY
    • Journal Title

      FEMS Immunology & Medical Microbiology

      Volume: 64(3) Pages: 425-428

    • DOI

      10.1099/jmm.0.054247-0

  • [Journal Article] Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease2012

    • Author(s)
      Yoneda M, Naka S, Nakano K, Wada K, Endo H, Mawatari H, Imajo K, Nomura R, Hokamura K, Ono M, Murata S, Tohnai I, Sumida Y, Shima T, Kuboniwa M, Umemura K, Kamisaki Y, Amano A, Okanoue T, Ooshima T, Nakajima A
    • Journal Title

      BMC Gastroenterology

      Volume: 12(1) Pages: 16

    • DOI

      10.1186/1471-230X-12-16

  • [Journal Article] BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex2012

    • Author(s)
      Kawai S, Amano A
    • Journal Title

      Journal of Cell Biology

      Volume: 197(2) Pages: 201-208

    • DOI

      10.1083/jcb.201110008

  • [Journal Article] Negative regulation of Odd-skipped related 2 by TGF-achieves the induction of cellular migration and the arrest of cell cycle2012

    • Author(s)
      Kawai S, Amano A
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 421(4) Pages: 696-700

    • DOI

      10.1016/j.bbrc.2012.04.064

  • [Journal Article] Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts2012

    • Author(s)
      Inaba H, Kuboniwa M, Sugita H, Lamont RJ, Amano A
    • Journal Title

      Infection and Immunity

      Volume: 80(8) Pages: 2847-2857

    • DOI

      10.1128/IAI.00258-12

  • [Journal Article] Distribution and molecular characterization of Porphyromonas gulae carrying a new fimA genotype2012

    • Author(s)
      Yamasaki Y, Nomura R, Nakano K, Inaba H, Kuboniwa M, Hirai N, Shirai M, Kato Y, Murakami M, Naka S, Iwai S, Matsumoto-Nakano M, Ooshima T, Amano A, Asai F
    • Journal Title

      Veterinary Microbiology

      Volume: 161(1) Pages: 196-205

    • DOI

      10.1016/j.vetmic.2012.07.026

  • [Journal Article] Insights into the virulence of oral biofilms : discoveries from proteomics2012

    • Author(s)
      Kuboniwa M, Tribble GD, Hendrickson EL, Amano A, Lamont RJ, Hackett M
    • Journal Title

      Expert Review of Proteomics

      Volume: 9(3) Pages: 311-323

    • DOI

      10.1586/epr.12.16

  • [Journal Article] SNARE mediates autophagosome-lysosome fusion2012

    • Author(s)
      Furuta N, Amano A
    • Journal Title

      Journal of Oral Bioscience

      Volume: 54(2) Pages: 83-85

  • [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy2012

    • Author(s)
      Klionsky DJ, Amano A, and 1268 others
    • Journal Title

      Autophagy

      Volume: 8(4) Pages: 1-100

  • [Journal Article] Cell entry and exit by periodontal pathogen Porphyromonas gingivalis2012

    • Author(s)
      Amano A, Furuta N
    • Journal Title

      Journal of Oral Bioscience

      Volume: 54(1) Pages: 54-57

  • [Journal Article] Proliferation of smooth muscle cells stimulated with Porphyromonas gingivalis is inhibited by apple polyphenol2011

    • Author(s)
      Inaba H, Tagashira M, Kanda T, Amano A
    • Journal Title

      Journal of Periodontology

      Volume: 82(11) Pages: 1616-1622

    • DOI

      10.1902/jop.2011.100785

  • [Journal Article] The collagen-binding protein of Streptococcus mutans is involved in hemorrhagic stroke2011

    • Author(s)
      Nakano K, Hokamura K, Taniguchi N, Wada K, Kudo C, Nomura R, Kojima A, Naka S, Muranaka Y, Thura M, Nakajima A, Masuda K, Nakagawa I, Speziale P, Shimada N, Amano A, Kamisaki Y, Tanaka T, Umemura K. Ooshima T
    • Journal Title

      Nature Communications

      Volume: 2 Pages: 485

    • DOI

      10.1038/ncomms1491

  • [Journal Article] Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway2011

    • Author(s)
      Takeuchi H, Furuta N, Morisaki I, Amano A
    • Journal Title

      Cellular Microbiology

      Volume: 13(5) Pages: 677-691

    • DOI

      10.1111/j.1462-5822.2010.01564.x

  • [Journal Article] Cellular ATP synthesis mediated by type III sodium-dependent phosphate transporter Pit-1 is critical to chondrogenesis2011

    • Author(s)
      Sugita A, Kawai S, Hayashibara T, Amano A, Ooshima T, Michigami T, Yoshikawa H, Yoneda T
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286(4) Pages: 3094-3103

    • DOI

      10.1074/jbc.M110.148403

  • [Journal Article] Characterization of aortic aneurysms in cardiovascular disease patients harboring Porphyromonas gingivalis2011

    • Author(s)
      Nakano K, Wada K, Nomura R, Nemoto H, Inaba H, Kojima A, Naka S, Hokamura K, Mukai T, Nakajima A, Umemura K, Kamisaki Y, Yoshioka H, Taniguchi K, Amano A, Ooshima T
    • Journal Title

      Oral Diseases

      Volume: 17(4) Pages: 370-378

    • DOI

      10.1111/j.1601-0825.2010.01759.x

  • [Journal Article] Cell entry and exit by periodontal pathogen via recycling pathway2011

    • Author(s)
      Takeuchi H, Furuta N, AmanoA
    • Journal Title

      Communicative & Integrative Biology

      Volume: 4(5) Pages: 587-589

    • DOI

      10.4161/cib.4.5.16549

  • [Presentation] Unique strategic system of Porphyromonas gingivalis in periodontitis2014

    • Author(s)
      Amano A
    • Organizer
      Kyudai Oral Bioscience 2014, 8th International Symposium
    • Place of Presentation
      Hakata
    • Year and Date
      2014-03-01
    • Invited
  • [Presentation] Molecular basis of periodontal destruction by periodontal bacteria2013

    • Author(s)
      Amano A
    • Organizer
      Exchange seminar between Osaka University and National Taiwan University
    • Place of Presentation
      Taipei
    • Year and Date
      2013-12-24
    • Invited
  • [Presentation] P. gingivalis の歯周組織破壊戦略-変貌する歯周病因論-サテライトシンポジウム10 歯周病菌による歯周組織破壊メカニズム : 現状と展望2013

    • Author(s)
      天野敦雄
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山市
    • Year and Date
      2013-09-20
    • Invited
  • [Presentation] Molecular basis of periodontal destruction by periodontal bacteria. JADR-sponsored symposium2013

    • Author(s)
      Amano A
    • Organizer
      2nd meeting of IADR-Asia Pacific Region
    • Place of Presentation
      Bangkok
    • Year and Date
      2013-08-21
    • Invited
  • [Presentation] 口腔から挑むBioResearch : Dental Science のときめき. シンポジウム「トレンドメーカーによるサイエンティフィック・エクスチェンジ」2013

    • Author(s)
      天野敦雄
    • Organizer
      第62回口腔衛生学会
    • Place of Presentation
      松本
    • Year and Date
      2013-05-16
    • Invited
  • [Presentation] Strategy of periodontal bacteria for periodontal destruction2013

    • Author(s)
      Amano A
    • Organizer
      Seminar at School of Dentistry University of Louisville
    • Place of Presentation
      Louisville, Kentucky
    • Year and Date
      2013-03-18
    • Invited
  • [Presentation] Strategy of periodontal bacteria for periodontal destruction2013

    • Author(s)
      Amano A
    • Organizer
      Seminar at Penn Dental
    • Place of Presentation
      Philadelphia, Pennsylvania
    • Year and Date
      2013-03-15
    • Invited
  • [Presentation] Strategy of P. gingivalis for periodontal destruction2012

    • Author(s)
      Amano A
    • Organizer
      First international conference on Porphyromonas gingivalis and related bacterial species
    • Place of Presentation
      Nagasaki
    • Year and Date
      2012-08-28
    • Invited
  • [Presentation] 基礎研究が指し示す歯周病因論自由集会5「歯科における健康を科学する」2012

    • Author(s)
      天野敦雄
    • Organizer
      第61回口腔衛生学会
    • Place of Presentation
      横須賀
    • Year and Date
      2012-05-27
    • Invited
  • [Presentation] 病因論から再考する歯周治療障害者歯科学会教育講座2011

    • Author(s)
      天野敦雄
    • Organizer
      第28回日本障害者歯科学会学術大会
    • Place of Presentation
      福岡
    • Year and Date
      2011-11-05
    • Invited
  • [Presentation] Outer membrane vesicles function as offensive weapons of Porphyromonas gingivalis2011

    • Author(s)
      Amano A
    • Organizer
      The Second Osaka Mahidol Chulalongkorn Joint Symposium
    • Place of Presentation
      Bangkok
    • Year and Date
      2011-10-25
    • Invited
  • [Presentation] Outer membrane vesicles function as offensive weapons of Porphyromonas gingivalis2011

    • Author(s)
      Amano A
    • Organizer
      13rd International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-03
    • Invited
  • [Presentation] Strategy of periodontal bacteria for periodontal destruction. Periodontik Terkini2011

    • Author(s)
      Amano A
    • Organizer
      Universitas Gadjah Mada
    • Place of Presentation
      Yogjakarta, Indonesia
    • Year and Date
      2011-07-04
    • Invited
  • [Book] 糖アルコールバイオフィルムへの効果 オーラルヘルスケア機能性食品の開発と応用 : アンチエイジングを目指した口腔ケアを中心に(坂上 宏 監修)2013

    • Author(s)
      橋野恵衣, 天野敦雄
    • Total Pages
      101-108
    • Publisher
      シーエムシー出版, 東京
  • [Book] Genotyping of periodontal anaerobic bacteria in relationship to pathogenesis. In de Filipis I, McKee ML (ed.), Molecular typing in bacterial infections2012

    • Author(s)
      Kuboniwa M, Amano A*
    • Total Pages
      149-166
    • Publisher
      Springer, NY, U.S.A
  • [Book] 歯周病の発症 ビジュアル歯周病を科学する(天野敦雄, 村上伸也, 岡賢二 編)2012

    • Author(s)
      天野敦雄
    • Total Pages
      12-32
    • Publisher
      クインテッセンス出版, 東京
  • [Book] 歯周組織内に生息する歯周病菌 ビジュアル 歯周病を科学する(天野敦雄, 村上伸也, 岡 賢二 編)2012

    • Author(s)
      天野敦雄
    • Total Pages
      139-154
    • Publisher
      クインテッセンス出版, 東京
  • [Book] 歯周病原菌red complex の宿主傷害戦略 ビジュアル 歯周病を科学する(天野敦雄, 村上伸也, 岡 賢二 編)2012

    • Author(s)
      天野敦雄
    • Total Pages
      155-180
    • Publisher
      クインテッセンス出版, 東京
  • [Book] オートファジーと感染・免疫オートファジー(水島 昇・吉森 保 編)2012

    • Author(s)
      天野敦雄
    • Total Pages
      150-164
    • Publisher
      化学同人, 京都
  • [Remarks]

    • URL

      http://web.dent.osaka-u.ac.jp/~prevent/

URL: 

Published: 2015-06-25  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi