2013 Fiscal Year Final Research Report
Involvement of phosphorylated L1-CAM in the plastic changes of nociecptive circuit following peripheral nerve injury
| Project/Area Number |
23500418
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 神経傷害性疼痛 / 脊髄後角 / 可塑性 / 細胞間接着因子 |
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| Research Abstract |
We examined whether peripheral nerve injury affects on the phosphorylation of L1-CAM in the rat DRG and spinal cord using a phospho-specific L1-CAM (Ser1181) antibody. Expression of phosphorylated L1-CAM was examined in rats (Sprague-Dawley, 250 g) that received tibial and common peroneal nerve ligation. Total and phosphorylated L1-CAM immunoreactivity (ir) were constitutively expressed in small un-myelinated DRG neurons. Nerve injury decreased total L1-CAM ir in the cytoplasm of somata in small neurons and increased it in cell membrane resulting to the formation of L1-CAM ir ring-like structures of in the injured small DRG neurons. Phosphorylated L1-CAM ir showed a dramatic decrease in the injured DRG. In dorsal horn, total L1-CAM showed the increase in the terminal of primary afferents horn. In contrast to the DRG, nerve injury increased the phosphorylated L1-CAM in the terminal of primary afferent in the dorsal horn .
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Research Products
(1 results)
