2013 Fiscal Year Final Research Report
The cell transplant system using improved TRECK mice
| Project/Area Number |
23500496
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
SAITO Michiko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40379558)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 再生医療 / 糖尿病 |
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| Research Abstract |
The TRECK method is very useful for generating model mice and obtaining useful donor cells. However, it has been difficult to make DTR expressed in whole bodies of mice because DTR is a growth factor and overexpression of it induces a slightly abnormal phenotype. In this study, to solve these problems, we generated improved TRECK mice using a modified DT receptor with reduced growth factor activity. The improved TRECK mice showed a normal phenotype and the DTR could be expressed in whole mice bodies. This improvement is expected to extend the applicable range of the TRECK method. Furthermore, we could demonstrate that transplanted and accepted donor cells from this mouse could be removed again from the recipient mouse by DT administration to examine the effect of the transplantation.
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[Journal Article] Generation of mouse models for type 1 diabetes by selective depletion of pancreatic beta cells using toxin receptor-mediated cell knockout2013
Author(s)
Matsuoka K, Saito M, Shibata K, Sekine M, Shitara H, Taya C, Xiaohong Zhang, Takahashi A. T., Kohno K, Kikkawa Y, Yonekawa H
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Journal Title
Biochemical and biophysical research communications
Volume: 436
Pages: 400-405
Peer Reviewed
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[Journal Article] Fetal exposure to 2, 3, 7, 8- tetrachlorodibenzo-p-dioxin transactivates aryl hydrocarbon receptor-responsive element III in the tyrosine hydroxylase immunoreactive neurons of the mouse midbrain2013
Author(s)
Tanida T, Tasaka K, Akahoshi E, Ishihara-Sugano M, Saito M, Kawata S, Danjo M, Tokumoto J, Mantani Y, Nagahara D, Tabuchi Y, Yokoyama T, Kitagawa H, Kawata M, Hoshi N
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Journal Title
Peer Reviewed
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[Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage2012
Author(s)
Kamimura R, Ishii T, Sasaki N, Kajiwara M, Machimoto T, Saito M, Kohno K, Suemori H, Nakatsuji N, Ikai I, Yasuchika K, and Uemoto S
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Journal Title
Cell Transplantation
Volume: 21(11)
Pages: 2351-2362
Peer Reviewed
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