Effects of polyunsaturated fatty acids in medium on cultured cardiomyocyte function

2013 Fiscal Year Final Research Report

• Project/Area Number: 23500539
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Yamagata University
• Principal Investigator: NAKAMURA Takao 山形大学, 医学(系)研究科(研究院), 教授 (00142654)
• Co-Investigator(Renkei-kenkyūsha): FENG Zhonggang 山形大学, 大学院・理工学研究科, 准教授 (10332545) ; NISHINA Atsuyoshi 日本大学, 理工学部, 教授 (00469884)
• Research Collaborator: KUSUNOKI Masataka 愛知医科大, 医学部, 准教授 (80214956) ; SATO Daisuke 山形大学, 大学院・医学系研究科, 助教 (60536960) ; TSUTSUMI Kazuhiko 徳島文理大学, 薬学部, 特任教授
• Project Period (FY): 2011 – 2013
• Keywords: 心筋細胞 / 培養 / 多価不飽和脂肪酸 / ラット

Research Abstract

Fatty acid (FA) compositions in fetal, neonatal, and cultured cardiomyocytes (CMs) were evaluated and compared to each other. The results suggest that cultured CMs have considerably lower amount of polyunsaturated FAs (PUFAs) than neonatal ones do. Thus, we added some PUFAs, which were combined with albumin, into medium, and found that FAs were successfully incorporated in CMs, that FA elongation may have taken place, and that the beating performance was tripled. In the next stage, FAs to add should be optimized to gain the best mechanical performance.

Research Products

• [Journal Article] Fatty acid composition in fetal, neonatal, and cultured cardiomyocytes in rats (2013)
  • Author(s): Karimata T, Seya D, Wakatsuki T, Feng Z, Sato D, Nishina A, Kusunoki M, Nakamura T
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: 49(10) Pages: 798-804
  • DOI: 10.1007/s11626-013-9668-3
  • Peer Reviewed
• [Journal Article] ラット培養心筋細胞の脂肪酸組成 (2012)
  • Author(s): 若槻峻, 瀬谷大貴, 佐藤大地, 佐藤大介, 狩俣徹, 仁科淳良, 楠正隆, 中村孝夫
  • Journal Title: 生体・生理工学シンポジウム論文集 Volume: 27 Pages: 446-447
• [Presentation] リノール酸添加培地がラット培養心筋細胞脂肪酸組成に与える影響 (2013)
  • Author(s): 若槻峻, 佐藤大地, 瀬谷大貴, 狩俣徹, 岡田研吾, 楠正隆, 仁科淳良, 馮忠剛, 佐藤大介, 中村孝夫
  • Organizer: 第51回日本人工臓器学会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2013-09-28
• [Presentation] Low contents of polyunsaturated fatty acid in cultured rat cardiomyocytes (2013)
  • Author(s): Sato D, Karimata T, Wakatsuki T, Feng Z, Nishina A, Kusunoki M, Nakamura
  • Organizer: MEDICON
  • Place of Presentation: Melia Sevilia (Seville, Spain)
  • Year and Date: 2013-09-27
• [Presentation] ラット培養心筋細胞の脂肪酸分布特性 (2012)
  • Author(s): 佐藤大地, 瀬谷大貴, 若槻峻, 狩俣徹, 岡田研吾, 楠正隆, 仁科淳良, 馮忠剛, 佐藤大介, 中村孝夫
  • Organizer: 第50回日本人工臓器学会
  • Place of Presentation: アクロス福岡(福岡)
  • Year and Date: 2012-11-23
• [Presentation] ラット培養心筋細胞の脂肪酸組成 (2012)
  • Author(s): 若槻峻, 瀬谷大貴, 佐藤大地, 佐藤大介, 狩俣徹, 仁科淳良, 楠正隆, 中村孝夫
  • Organizer: 第27回生体・生理工学シンポジウム
  • Place of Presentation: 北海道大学学術交流会館(札幌)
  • Year and Date: 2012-09-21
• [Presentation] 胎児及び新生児ラット心筋組織の脂肪酸分布特性 (2011)
  • Author(s): 佐藤大地, 瀬谷大貴, 狩俣徹, 馮忠剛, 中村孝夫
  • Organizer: 第49回日本人工臓器学会
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 2011-11-26
• [Book] Low contents of polyunsaturated fatty acid in cultured rat cardiomyocytes (2013)
  • Author(s): Sato D, Karimata T, Wakatsuki T, Feng Z, Nishina A, Kusunoki M, Nakamura T
  • Total Pages: 907-910
  • Publisher: Springer (Heidelberg)