Swallowing dysfunction model:Examination of swallowing function by afferent stimulation

2013 Fiscal Year Final Research Report

• Project/Area Number: 23500640
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Tohoku University
• Principal Investigator: MITSUHIRO Kano 東北大学, 歯学研究科(研究院), 大学院非常勤講師 (10419236)
• Co-Investigator(Renkei-kenkyūsha): ICHIKAWA Hiroyuki 東北大学, 大学院・歯学研究科, 教授 (20193435)
• Project Period (FY): 2011 – 2013
• Keywords: 嚥下機能 / リハビリテーション

Research Abstract

Damage of sensory neurons innervating the pharynx can cause swallowing dysfunction in animal models. In this study, distribution of sensory nerve fibers was examined in the rat epiglottis and pharynx by immunohistochemical methods. Pituitary adenylate cyclase-activating polypeptide-immunoreactive (PACAP-IR) nerve fibers were located beneath the mucous epithelium, and occasionally penetrated the epithelium. PACAP-IR nerve fibers were also detected in taste buds within the epiglottis and pharynx. Retrograde tracing method also demonstrated that PACAP was expressed by vagal and glossopharyngeal sensory neurons innervating the pharynx. These findings suggest that PACAP-IR nerve fibers in the epithelium and taste bud of the epiglottis and pharynx which originate from the vagal and glossopharyngeal sensory ganglia include nociceptors and chemoreceptors. Injury of PACAP-IR neurons innervating the pharynx and epiglottis may cause swallowing dysfunction.

Research Products

• [Journal Article] The distribution of galaninimmunoreactive nerve fibers in the rat pharynx (2013)
  • Author(s): Toshihiko Suzuki, Tadasu Sato, Mitsuhiro Kano and Hiroyuki Ichikawa
  • Journal Title: Neuropeptides Volume: 47(4) Pages: 231-236
  • DOI: 10.1016/j.nep.2013.05.001
  • Peer Reviewed
• [Journal Article] The distribution of TRPV1 and TRPV2 in the rat pharynx (2013)
  • Author(s): Rika Sasaki, Tadasu Sato, Takehiro Yajima, Mitsuhiro Kano, Toshihiko Suzuki and Hiroyuki Ichikawa
  • Journal Title: Cellular and Molecular Neurobiology Volume: 33(5) Pages: 707-714
  • DOI: 10.1007/s10571-013-9938-3
  • Peer Reviewed
• [Journal Article] The Distribution of Transient Receptor Potential Melastatin-8 in the Rat Soft Palate, Epiglottis, and Pharynx (2013)
  • Author(s): Tadasu Sato, Masatoshi Fujita, Mitsuhiro Kano, Hiroshi Hosokawa, Teruyoshi Kondo, Toshihiko Suzuki, Eriko Kasahara, Noriaki Shoji, Takashi Sasano and Hiroyuki Ichikawa
  • Journal Title: Cellular and Molecular Neurobiology Volume: 33(2) Pages: 161-165
  • DOI: 10.1007/s10571-012-9888-1
  • Peer Reviewed
• [Presentation] ラット咽頭及びその周囲組織におけるTRPV1とTRPV2の分布 (2013)
  • Author(s): 佐藤匡, 矢島健大, 狩野充浩, 鈴木敏彦, 市川博之
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山
  • Year and Date: 20130920-22
• [Presentation] ラット軟口蓋, 喉頭蓋及び咽頭におけるTRPM8, TRPV1, TRPV2の分布 (2013)
  • Author(s): 佐藤匡, 佐々木里香, 藤田雅俊, 狩野充浩, 澤浦亮平, 鈴木敏彦, 市川博之
  • Organizer: 日本解剖学会第59回東北・北海道連合支部学術集会
  • Place of Presentation: 札幌
  • Year and Date: 20130914-15
• [Presentation] THE DISTRIBUTION OF TRPV1 AND TRPV2 IN THE RAT PHARYNX (2013)
  • Author(s): Tadasu Sato, Rika Sasaki, Mitsuhiro Kano, Toshihiko Suzuki, and Hiroyuki Ichikawa
  • Organizer: ASahct2013 International of Symposium Anatomical Science for advance in health and clinical therapy
  • Place of Presentation: 仙台
  • Year and Date: 20130827-28
• [Presentation] ラット軟口蓋, 喉頭蓋及び咽頭におけるTRPM8の分布 (2012)
  • Author(s): 佐藤匡, 藤田雅俊, 狩野充浩, 鈴木敏彦, 市川博之
  • Organizer: 第54回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 郡山
  • Year and Date: 20120914-16
• [Presentation] Dmuマウスにおける骨格筋及び運動ニューロンの変性 (2012)
  • Author(s): 藤田雅俊, 佐藤匡, 狩野充浩, 清水良央, 金高弘恭, 鈴木敏彦, 市川博之
  • Organizer: 第54回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 郡山
  • Year and Date: 20120914-16