2013 Fiscal Year Final Research Report

CEHC is the active form of non alpha-tocoperol in vivo

Research Project

Project/Area Number	23500972
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Eating habits, studies on eating habits
Research Institution	Kanagawa Institute of Technology
Principal Investigator	KIYOSE Chikako 神奈川工科大学, 応用バイオ科学部, 教授 (50272745)
Project Period (FY)	2011 – 2013
Keywords	ビタミンＥ / CEHC / 脂肪細胞 / 炎症性サイトカイン / 共培養法 / アロエ抽出物
Research Abstract	Vitamin E has eight different naturally occurring forms: four tocopherols (Toc) and four tocotrienols (Toc-3), among which alha-Toc, in particular, is knowns to be a major antioxidant for protection fo cellular membranes. Vitamin E analogs are metabolized to carboxyethyl-hydroxychroman (CEHC) in vivo and excreted into urine. Although the CEHC form is the main metabolite of vitamin E, CEHC -particularly gamma-CEHC- has also been reported to have some physiological functions, for example as a natriuretic hormone and an antioxidant. Therefore, I investigated the effect of vitamin E and its metabolite on the adipocytes induced inflammation. At first, I determined the culture method of adipocytes (3T3-L1 cells) with macrophages (RAW264.7 cells). Next, I examined the effect of vitamin E on the adipocytes induced inflammation. According to this result, I was shown that gamma-tocotorienol could not suppress the inflammation of adipocytes.