2013 Fiscal Year Final Research Report
Functinal analysis of a novel gene involved in the progression of colorectal cancers
| Project/Area Number |
23501276
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Japanese Foundation for Cancer Research |
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Principal Investigator |
NAGAYAMA satoshi 公益財団法人がん研究会, その他部局等, その他 (70362499)
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| Co-Investigator(Kenkyū-buntansha) |
戸口田 淳也 京都大学, 再生医科学研究所/iPS 細胞研究所, 教授/副所長 (40273502)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 大腸癌 / 浸潤 |
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| Research Abstract |
In this study, we found a marked elevation of AFAP1L1 gene expression in colorectal cancer (CRC) tissues as compared to the adjacent normal mucosa. Multivariate analysis revealed that AFAP1L1 was an independent and significant factor for the recurrence of rectal cancers. AFAP1L1-transduced CRC cells exhibited a rounded shape, increased cell motility on planar substrates, and resistance to anoikis in vitro. AFAP1L1 was identified as a novel associating partner of vinculin by immunoprecipitation assay. The local administration of a siRNA against AFAP1L1 significantly suppressed the in vivo tumor growth of xenografts, suggesting that AFAP1L1 might be a candidate therapeutic target for CRCs. These results suggest that AFAP1L1 plays a role in the progression of CRCs by modulating cell shape and motility and by inhibiting anoikis, presumably through interactions with vinculin-including protein complexes.
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