2013 Fiscal Year Final Research Report
Enhancement of drug-sensitivity through the reactivation of mutant p53
Project/Area Number |
23501278
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor biology
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Research Institution | Chiba Cancer Center (Research Institute) |
Principal Investigator |
OZAKI TOSHINORI 千葉県がんセンター(研究所), その他部局等, 研究員 (40260252)
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Project Period (FY) |
2011 – 2013
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Keywords | がん抑制遺伝子 |
Research Abstract |
We have identified co-chaperon human DnaJC7 as one of the binding partners of mutant p53. According to our immunoprecipitation experiments, DnaJC7 was associated with mutant p53 as well as wild-type p53 in mammalian cultured cells. Of note, DnaJC7 promoted the dissociation of MDM2 from wild-type p53, and thereby extended its half-life and stimulated its transcriptional as well as pro-apoptotic activity. In addition, luciferase reporter assays demonstrated that DnaJC7 has an ability to reduce the inhibitory effect of mutant p53 on wild-type p53. Taken together, our present results strongly suggest that DnaJC7 might enhance the drug-sensitivity of malignant cancerous cells through the physical and functional interactions with wild-type p53 and/or mutant p53.
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Research Products
(8 results)
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[Journal Article] Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer Pyrrole-Imidazole polyamide targeting E-box2014
Author(s)
Taniguchi M, Fujiwara K, Nakai Y, Ozaki T, Koshikawa N, Kojima T, Kataba M, Oguni A, Matsuda H, Yoshida Y, Tokuhashi Y, Fukuda N, Ueno T, Soma M, and Nagase H
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Journal Title
FEBS Open Bio
Volume: 4
Pages: 328-334
Peer Reviewed
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[Journal Article] Receptor-type protein tyrosine phosphatase к (PTPRK) directly dephosphorylates CD133 and regulates downstream AKT activation2014
Author(s)
Shimozato O, Waraya M, Nakashima K, Soda H, Takiguchi N, Yamamoto H, Takenobu H, Uehara H, Ikeda E, Matsushita S, Kubo N, Nakagawara A, Ozaki T, Kamijo T
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Journal Title
Oncogene
Volume: (in press)
Peer Reviewed
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[Journal Article] Dependence receptor UNC5D mediates nerve growth factor depletion-induced neuroblastoma regression2013
Author(s)
Zhu Y, Li Y, Haraguchi S, Yu M, Ohira M, Ozaki T, Nakagawara A, Ushijima T, Isogai E, Koseki H, Nakamura Y, Kong C, Mehlen P, Arakawa H, Nakagawara A
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Journal Title
J. Clin. Invest.
Volume: 123
Pages: 2935-2947
Peer Reviewed
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