Cell lineage-related mechanisms involved in lung cancer stem cell development.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23501281
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: OSADA Hirotaka 愛知県がんセンター(研究所), 分子腫瘍学部, 室長 (30204176)
• Project Period (FY): 2011 – 2013
• Keywords: 肺がん / 幹細胞

Research Abstract

Sphere-forming cells were initially established from several different lung cancer cell lines, after which expression profiling and tumorigenesis assay findings indicated their cancer stem cell properties. In addition, expression of neuroendocrine-related genes in these cells suggests a possible association between sphere formation and cell lineage plasticity. Furthermore, neuroendocrine-inducing ASH1 has also been found to promote sphere formation in some cell lines, suggesting the involvement of ASH1 signaling in development of cancer stem cells.

Research Products

• [Journal Article] CD74-NRG1 fusions in lung adenocarcinoma (2014)
  • Author(s): Fernandez-Cuesta L, Plenker D, Osada H, Sun R, Menon R, Leenders F, Ortiz-Cuaran S, Peifer M, Bos M, DaBler J, Malchers F, Schottle J, Vogel W, Dahmen I, Koker M, Ullrich RT, Wright GM, Russell PA, Wainer Z, Solomon B, Brambilla E, Nagy-Mignotte H, Moro-Sibilot D, Brambilla CG, Lantuejoul S, Altmuller J, Becker C, Nurnberg P, Heuckmann JM, Stoelben E, Petersen I, Clement JH, Sanger J, Muscarella LA, la Torre A, Fazio VM, Lahortiga I, Perera T, Ogata S, Parade M, Brehmer D, Vingron M, Heukamp LC, Buettner R, Zander T, Wolf J, Perner S, Ansen S, Haas SA, Yatabe Y, Thomas RK
  • Journal Title: Cancer Discovery Volume: 4 Pages: 415-422
  • Peer Reviewed
• [Journal Article] RASSF3 downregulation increases malignant phenotypes of non-small cell lung cancer (2014)
  • Author(s): Fukatsu A, Ishiguro F, Tanaka I, Kudo T, Nakagawa K, Shinjo K, Kondo Y, Fujii M, Hasegawa Y, Tomizawa K, Mitsudomi T, Osada H, Hata Y, Sekido Y
  • Journal Title: Lung Cancer Volume: 83 Pages: 23-29
  • Peer Reviewed
• [Journal Article] Hepatitis virus infection affects DNA methylation in mice with humanized livers (2014)
  • Author(s): Okamoto Y, Shinjo K, Shimizu Y, Sano T, Yamao K, Gao W, Fujii M, Osada H, Sekido Y, Murakami S, Tanaka Y, Joh T, Sato S, Takahashi S, Wakita T, Zhu J, Issa JP, Kondo Y
  • Journal Title: Gastroenterology Volume: 146 Pages: 562-572
  • Peer Reviewed
• [Journal Article] Membranous expression of activated leukocyte cell adhesion molecule contributes to poor prognosis and malignant phenotypes of non-small-cell lung cancer (2013)
  • Author(s): Ishiguro F, Murakami H, Mizuno T, Fujii M, Kondo Y, Usami N, Taniguchi T, Yokoi K, Osada H, Sekido Y
  • Journal Title: J Surg Res Volume: 179 Pages: 24-32
  • Peer Reviewed
• [Journal Article] NKX2-1/TITF1/TTF-1-Induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma (2012)
  • Author(s): Yamaguchi T, Yanagisawa K, Sugiyama R, Hosono Y, Shimada Y, Arima C, Kato S, Tomida S, Suzuki M, Osada H, Takahashi T
  • Journal Title: Cancer Cell Volume: 21 Pages: 348-361
  • Peer Reviewed
• [Journal Article] YAP induces malignant mesothelioma cell proliferation by upregulating transcription of cell cycle promoting genes (2012)
  • Author(s): Mizuno T, Murakami H, Fujii M, Ishiguro F, Tanaka I, Kondo Y, Akatsuka S, Toyokuni S, Yokoi K, Osada H, Sekido Y
  • Journal Title: Oncogene Volume: 31 Pages: 5117-5122
  • Peer Reviewed
• [Journal Article] Activated leukocyte cell adhesion molecule (ALCAM) promotes malignant phenotypes of malignant mesothelioma (2012)
  • Author(s): Ishiguro F, Murakami H, Mizuno T, Fujii M, Kondo Y, Usami N, Yokoi K, Osada H, Sekido Y
  • Journal Title: J Thorac Oncol Volume: 7 Pages: 890-899
  • Peer Reviewed
• [Journal Article] TGF-β synergizes with defects in the Hippo pathway to stimulate human malignant mesothelioma growth (2012)
  • Author(s): Fujii M, Toyoda T, Nakanishi H, Yatabe Y, Sato A, Matsudaira Y, Ito H, Murakami H, Kondo Y, Kondo E, Hida T, Tsujimura T, Osada H, Sekido Y
  • Journal Title: J Exp Med Volume: 209 Pages: 479-494
  • Peer Reviewed
• [Journal Article] Integrated analysis of genetic and epigenetic alterations reveals CpG island methylator phenotype associated with distinct clinical characters of lung adenocarcinoma (2012)
  • Author(s): Shinjo K, Okamoto Y, An B, Yokoyama T, Takeuchi I, Fujii M, Osada H, Usami U, Hasegawa Y, Ito H, Hida T, Fujimoto N, Kishimoto T, Sekido S, Kondo Y
  • Journal Title: Carcinogenesis Volume: 33 Pages: 1277-1285
  • Peer Reviewed
• [Journal Article] Contribution of microRNA-1275 to Claudin 11 expression via polycomb-mediated silencing mechanism in human glioma stem-like cells (2012)
  • Author(s): Katsushima K, Shinjo K, Natsume A, Ohka F, Fujii F, Osada H, Sekido Y, Kondo Y
  • Journal Title: J Biol Chem Volume: 287 Pages: 27396-27406
  • Peer Reviewed
• [Journal Article] Conversion from the "oncogene addiction" to "drug addiction" by intensive inhibition of the EGFR and MET in lung cancer with activating EGFR mutation (2012)
  • Author(s): Suda K, Tomizawa K, Osada H, Maehara Y, Yatabe Y, Sekido Y, Mitsudomi T
  • Journal Title: Lung Cancer Volume: 76 Pages: 292-299
  • Peer Reviewed
• [Journal Article] The circadian clock gene BMAL1 is a novel therapeutic target for malignant mesothelioma (2012)
  • Author(s): Elshazley M, Sato M, Hase T, Takeyama Y, Yamashita R, Yoshida K, Toyokuni S, Ishiguro F, Osada H, Sekido Y, Yokoi K, Usami N, Shames DS, Kondo M, Gazdar AF, Minna JD, Hasegawa Y
  • Journal Title: Int J Cancer Volume: 131 Pages: 2820-2831
  • Peer Reviewed
• [Journal Article] Aberrant DNA methylation associated with aggressiveness of gastrointestinal stromal tumour (2012)
  • Author(s): Okamoto Y, Sawaki A, Ito S, Nishida T, Takahashi T, Toyota M, Suzuki H, Shinomura Y, Takeuchi I, Shinjo K, An B, Ito H, Yamao K, Fujii M, Murakami H, Osada H, Kataoka H, Joh T, Sekido Y, Kondo Y
  • Journal Title: GUT Volume: 61 Pages: 392-401
  • Peer Reviewed
• [Journal Article] let-7 and miR-17-92 : Small-sized major players in lung cancer development (2011)
  • Author(s): Osada H and Takahashi T
  • Journal Title: Cancer Sci Volume: 102 Pages: 9-17
  • Peer Reviewed
• [Journal Article] miR-375 is activated by ASH1 and inhibits YAP1 in a lineage dependent manner in lung cancermiR-375 is activated by ASH1 and inhibits YAP1 in a lineage dependent manner in lung cancer (2011)
  • Author(s): Nishikawa E, Osada H, Okazaki Y, Arima C, Tomida S, Tatematsu Y, Taguchi A, Shimada Y, Yanagisawa K, Yatabe Y, Toyokuni S, Sekido Y, Takahashi T
  • Journal Title: Cancer Res Volume: 71 Pages: 6165-6173
  • Peer Reviewed
• [Journal Article] LATS2 is a tumor suppressor gene of malignant mesothelioma (2011)
  • Author(s): Murakami H, Mizuno T, Taniguchi T, Fujii M, Ishiguro F, Fukui T, Akatsuka S, Horio Y, Hida T, Kondo Y, Toyokuni S, Osada H, Sekido Y
  • Journal Title: Cancer Res Volume: 71 Pages: 873-883
  • Peer Reviewed
• [Journal Article] Epithelial to mesenchymal transition in an EGFR-mutant lung cancer cell line with acquired resistance to erlotinib (2011)
  • Author(s): Suda K, Tomizawa K, Fujii M, Murakami H, Osada H, Maehara Y, Yatabe Y, Sekido Y, Mitsudomi T
  • Journal Title: J Thorac Oncol Volume: 6 Pages: 1152-1161
  • Peer Reviewed
• [Journal Article] Distinct profiles of epigenetic evolution between colorectal cancers with and without metastasis (2011)
  • Author(s): Ju HX, An B, Okamoto Y, Shinjo K, Kanemitsu Y, Komori K, Hirai T, Shimizu Y, Sano T, Sawaki A, Tajika M, Yamao K, Fujii M, Murakami H, Osada H, Ito H, Takeuchi I, Sekido Y, Kondo Y
  • Journal Title: Am J Pathol Volume: 178 Pages: 1835-1846
  • Peer Reviewed
• [Journal Article] LIM-domain protein AJUBA suppresses malignant mesothelioma cell proliferation via Hippo signaling cascade
  • Author(s): Tanaka I, Osada H, Fujii M, Fukatsu A, Hida T, Horio Y, Kondo Y, Sato A, Hasegawa Y, Tsujimura T, Sekido Y
  • Journal Title: Oncogene Volume: (in press)
  • Peer Reviewed
• [Presentation] Analysis of CLCP1, a potential target for lung cancer diagnostics and therapeutics (2013)
  • Author(s): Osada H, Yagi K, Yanagisawa K, Akatsuka J, Tatematsu Y, Kato S, Yatabe Y, Ono K, Sekido Y, Takahashi T
  • Organizer: 36th Molecular Biology Society of Japan
  • Place of Presentation: Kobe (Poster Session)
  • Year and Date: 20131203-06
• [Presentation] Functional analysis of CLCP1 as potential target for lung cancer diagnostics and therapeutics (2013)
  • Author(s): Osada H, Yagi K, Yanagisawa K, Akatsuka J, Tatematsu Y, Kato S, Yatabe Y, Ono K, Sekido Y, Takahashi T
  • Organizer: 72nd Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama (Oral Session)
  • Year and Date: 20131003-05
• [Presentation] Functional analysis of CLCP1 as a potential target for lung cancer diagnostics and therapeutics (2012)
  • Author(s): Osada H, Yagi K, Yanagisawa K, Akatsuka J, Tatematsu Y, Kato S, Yatabe Y, Ono K, Sekido Y, Takahashi T
  • Organizer: 71st Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Sapporo (Oral Session)
  • Year and Date: 20120919-21
• [Presentation] Roles of ASH1- regulated miRNAs and lincRNAs in lung cancer development (2011)
  • Author(s): Osada H, Takahashi T
  • Organizer: 70th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Nagoya (Symposium)
  • Year and Date: 20111003-05
• [Presentation] Roles of ASH1-miR-375 pathway in development of lung cancers with neuroendocrine features (2011)
  • Author(s): Osada H, Nishikawa E, Arima C, Okazaki Y, Tomida S, Tatematsu Y, Taguchi A, Shimada Y, Yanagisawa K, Toyokuni S, Sekido Y, Takahashi, T
  • Organizer: 102nd Annual Meeting of the American Association for Cancer Research
  • Place of Presentation: Orlando, USA (Poster)
  • Year and Date: 20110402-06
• [Book] microRNA 発現異常と発癌 実験医学増刊 29 (2) (2011)
  • Author(s): 長田 啓隆、高橋 隆
  • Total Pages: 56-61
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/03bunshi_shuyo/index.html