UVA activated N-nitrosoproline induced damages on DNA, protein and membrane, and disturbance of signal transduction

2013 Fiscal Year Final Research Report

• Project/Area Number: 23510075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Okayama University
• Principal Investigator: ARIMOTO Sakae 岡山大学, 医歯(薬)学総合研究科, 准教授 (90212654)
• Project Period (FY): 2011 – 2013
• Keywords: ニトロソプロリン / UVA / DNA損傷 / 一酸化窒素 / アルキルラジカル / DNA付加体 / 遺伝毒性 / 小核形成

Research Abstract

We investigated near-ultraviolet radiation (UVA) mediated chemical reaction of N-nitrosoproline (NPRO) with DNA, deoxynucleosides, amino acids and membrane lipids, and isolated several new products from the UVA-irradiated mixture. Photogenotoxicity was found with NPRO towards human derived keratinocyte cells. Nitric oxide was released during photoreaction of NPRO to create active radicals that can give phototoxicity.

Research Products

• [Journal Article] Mutagenicity and NO formation from UVA irradiated N-nitrosoproline, and characterization of photoproduct formed in UVA-irradiated mixture of N-nitrosoproline with 2'-deoxyguanosine in neutral solution (2014)
  • Author(s): Arimoto-Kobayashi, S., Machida, M., Sano, K., Aoyama, S., Asahi, C., Tanaka, N., Okamoto, K., Negishi, T., Kimura, S. and Suzuki, T
  • Journal Title: Genes and Environment Volume: Vol. 36(2)
  • DOI: 10.3123/jemsge.2014.004
  • Peer Reviewed
• [Journal Article] Near ultraviolet radiation-mediated reaction between N-nitrosoproline and DNA : Isolation and identification of two new adducts, (R)-and (S)-8-(2-pyrrolidyl)-2'-deoxyguanosine (2013)
  • Author(s): Aoyama, S., Sano, K., Asahi, C., Masaki Machida, Okamoto, K., Negishi, T., Kimura, S., Suzuki, T., Hatano, T. and Arimoto-Kobayashi, S
  • Journal Title: Journal of Photochemistry and Photobiology A : Chemistry Volume: Vol. 276 Pages: 1-7
  • DOI: 10.1016/j.jphotochem.2013.11.009
  • Peer Reviewed
• [Presentation] Release of nitric oxide and formation of mutagenic substance from UVA irradiated N-nitrosoproline in non-aqueous lipids (2014)
  • Author(s): Arimoto-Kobayashi,S., Moriwaki, N., Tanaka, N., Okamoto, K. and Negishi, T
  • Organizer: 17th Biennial Meeting of Society for Free Radical Research International (SFRRI2014)
  • Place of Presentation: 京都
  • Year and Date: 20140323-26
• [Presentation] 日本環境変異原学会シンポジウムII「光遺伝毒性の最近の動向」 (2013)
  • Author(s): 有元佐賀惠
  • Organizer: 「変異原性物質とDNA の光化学反応」、日本環境変異原学会42回大会
  • Place of Presentation: 岡山
  • Year and Date: 20131129-30
  • Invited
• [Presentation] Near ultraviolet light-mediated reaction between N-nitrosoproline and DNA : Isolation and identification of new adducts (2013)
  • Author(s): Arimoto-Kobayashi, S., Aoyama, S., Asahi, C., Sano, K., Machida, M., Okamoto, K., Negishi, T., Kimura, S., Suzuki, T. and Hatano, T
  • Organizer: The 6th Asia and Oceania Conference on Photobiology
  • Place of Presentation: シドニー
  • Year and Date: 20131110-13
• [Presentation] UVA 活性化N-ニトロソプロリンによるヒトケラチノサイト由来細胞への遺伝毒性 (2013)
  • Author(s): 田中範子、森脇直史、岡本敬の介、根岸友恵、佐藤聡、有元佐賀惠
  • Organizer: 第35回日本光医学・光生物学会
  • Place of Presentation: 浜松
  • Year and Date: 20130712-13
• [Presentation] N-ニトロソプロリンのUVA 光反応によるDNA 付加体形成 (2013)
  • Author(s): 旭千春、青山周平、岡本敬の介、根岸友恵、波多野力、有元佐賀惠
  • Organizer: 第35回日本光医学・光生物学会
  • Place of Presentation: 浜松
  • Year and Date: 20130712-13
• [Presentation] UVA 活性化N-ニトロソプロリンによる新規核酸付加体 (2012)
  • Author(s): 有元佐賀惠、青山周平、田中範子、佐野嘉容子、根岸友恵、波多野力、鈴木利典、木村幸子
  • Organizer: 第34回日本光医学・光生物学会
  • Place of Presentation: 神戸
  • Year and Date: 20120727-28
• [Presentation] 培養ヒトケラチノサイトによる化学物質の光細胞毒性・光遺伝毒性試験 (2011)
  • Author(s): 堀ノ内眞弓、有元佐賀惠
  • Organizer: 日本環境変異原学会第40回大会
  • Place of Presentation: 東京
  • Year and Date: 20111121-22
• [Presentation] Analysis of photoproducts from a UVA-irradiated mixture of N-nitrosoproline with 2'-deoxyadenosine or 2'-deoxyguanosine (2011)
  • Author(s): Aoyama, S., Sano, K., Hatano, T., Kimura, S. and Arimoto-Kobayashi, S
  • Organizer: The 5th Asia and Oceania Conference on Photobiology
  • Place of Presentation: 奈良
  • Year and Date: 20110730-0801
• [Presentation] N-ニトロソプロリンの光活性化による核酸修飾 (2011)
  • Author(s): 青山周平、佐野嘉容子,波多野力、木村幸子、有元佐賀惠
  • Organizer: 第33回日本光医学・光生物学会
  • Place of Presentation: 大阪
  • Year and Date: 20110722-23