Studies on the molecular mechanism of 5-fluorouracil response proteins using a proteomic approach

2014 Fiscal Year Final Research Report

• Project/Area Number: 23510267
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Osaka Medical College
• Principal Investigator: SAKAI Akiko 大阪医科大学, 医学部, 講師 (30225750)
• Co-Investigator(Kenkyū-buntansha): TANAKA Satoru 大阪医科大学, 医学部, 助教 (50595741)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 抗癌剤耐性 / プロテオミクス / HSPB1 / オリゴマー化 / サイトケラチン

Outline of Final Research Achievements

The heat-shock protein (HSP) B1 expression increased with the acquisition of 5-fluorouracil resistance. We analyzed the phosphorylation and oligomeric structure of HSPB1 by one- and two-dimensional western blotting via isoelectric focusing and blue native polyacrylamide gel electrophoresis. The oligomeric structures of HSPB1 in resistant cells differed in molecular mass from those in sensitive cells, suggesting that drug resistance alters the phosphorylation and dissociability of HSPB1 oligomers. Further, cytokeratin 8, 18, 19 were identified the proteins that interact with HSPB1 by co-immunoprecipitation.

Free Research Field

生物学