2013 Fiscal Year Final Research Report
Chemical tools specifc for auxin polar transport
| Project/Area Number |
23510285
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Okayama University of Science |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NOZAKI Hiroshi 岡山理科大学, 理学部, 教授 (60159085)
KOSHIBA Tomokazu 首都大学東京, 理工学研究科, 教授 (80117704)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 植物ホルモン / オーキシン / イメージング |
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| Research Abstract |
The auxin polar transport establish an asymmetric auxin distribution to respond environmental stimulus and consequently modulate the auxin responses of cells. Therefore, the auxin transport system plays a crucial role in plant development. The distribution of cellular auxin is regulated by auxin influx and efflux carriers/transporters localized in plant cell membrane. Therefore, the dynamic of intracellular auxin distribution are important for the understanding of molecular mechanism for auxin transport system. In this study, we developed the new chemical tools for the visualization and modulation of auxin gradient, such as fluorescent auxin and caged auxin. Fluorescent auxin analogs can visualize the distribution of auxin, and caged auxin can preciously modulate cellular auxin level. Both chemical tools can be promising to dissect the mechanism for the formation of auxin gradient.
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[Journal Article] Yucasin is a potent inhibitor of YUCCA, a key enzyme in auxin biosynthesis2014
Author(s)
Nishimura T, Hayashi K, Suzuki H, Gyohda A, Takaoka C, Sakaguchi Y, Matsumoto S, Kasahara H, Sakai T, Kato J, Kamiya Y, Koshiba T
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Journal Title
Plant J
Volume: 77(3)
Pages: 352-366
Peer Reviewed
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[Journal Article] Identification of IAA transport inhibitors including compounds affecting cellular PIN trafficking by two chemical screening approaches using maize coleoptile systems2012
Author(s)
Nishimura T, Matano N, Morishima T, Kakinuma C, Hayashi K, Komano T, Kubo M, Hasebe M, Kasahara H, Kamiya Y, Koshiba T
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Journal Title
Plant Cell Physiol
Volume: 53(10)
Pages: 1671-1682
Peer Reviewed
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[Journal Article] Transcription of DWARF4 plays a crucial role in auxin-regulated root elongation in addition to brassinosteroid homeostasis in Arabidopsis thaliana2011
Author(s)
Yoshimitsu Y, Tanaka K, Fukuda W, Asami T, Yoshida S, Hayashi K, Kamiya Y, Jikumaru Y, Shigeta T, Nakamura Y, Matsuo T, Okamoto S
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Journal Title
PLoS One
Volume: 6(8)
Pages: e23851
Peer Reviewed
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[Journal Article] Alkoxy-auxins are selective inhibitors of auxin transport mediated by PIN, ABCB, and AUX1 transporters2011
Author(s)
Tsuda E, Yang H, Nishimura T, Uehara Y, Sakai T, Furutani M, Koshiba T, Hirose M, Nozaki H, Murphy AS, Hayashi K
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Journal Title
J Biol Chem
Volume: 286(3)
Pages: 2354-2364
Peer Reviewed
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[Journal Article] The main auxin biosynthesis pathway in Arabidopsis2011
Author(s)
Mashiguchi K, Tanaka K, Sakai T, Sugawara S, Kawaide H, Natsume M, Hanada A, Yaeno T, Shirasu K, Yao H, McSteen P, Zhao Y, Hayashi K, Kamiya Y, Kasahara H
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Journal Title
Proc Natl Acad Sci USA
Volume: 108(45)
Pages: 18512-18517
Peer Reviewed
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