2013 Fiscal Year Final Research Report
Development of photochemical cloning for DNA-binding proteins
| Project/Area Number |
23510286
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHTSUKA Isao 九州保健福祉大学, 薬学部, 准教授 (20389589)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 光反応 / 分析化学 / コンフォメーション病 / DNA結合タンパク質 / ゲルシフトアッセイ |
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| Research Abstract |
We developed the new method for analysis of DNA-binding proteins, which play the various physiological roles such as transcriptional regulation, using photo-reactive compound, which is able to form the covalent bond by light irradiation. Our photochemical gel-shift assay reveals that octamer DNA binds several proteins of nuclear extract. We further examined the photochemical cloning methods combining with molecular biological technique, phage panning or instrumental analysis of mass spectrometry. We also analyzed expression pattern and fibrillation of cranial nerve disease, which is a target disease for photochemical methods in this study.
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