2013 Fiscal Year Final Research Report
Enhancement by liposome of semi-synthesis of specifically modified protein at the C-terminal and application to living cell
| Project/Area Number |
23550195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TERUYA Kenta 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (30372288)
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| Project Period (FY) |
2011 – 2013
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| Keywords | リポソーム / 縮合 / 蛋白質 / 細胞表面 |
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| Research Abstract |
In our previous study, semi-synthesis of protein specifically modified with lipo-philic compounds was carry out, but not effective. Denaturing protein during the specific condensation, purification of coupling product, and toxicity to live cell are to be adapted for improving effectively. To overcome these difficulties, liposome was employed in each step. As results, separation of coupling product from reaction mixture of condensation, and condensation avoiding denature of protein were carried out smoothly. However, use of liposome in coupling without detergent was not effective. Andmore, release of protein from the liposeme was not effective when liposeme horboring modified protein was applied to living cell.
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[Journal Article] Site-specific isotope labeling of long RNA for structural and mechanistic studies2012
Author(s)
Kawahara I, Haruta K, Ashihara Y, Yamanaka D, Kuriyama M, Toki N, Kondo Y, Teruya K, Ishikawa J, Furuta H, Ikawa Y, Kojima C, Tanaka Y
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Journal Title
Nucleic Acids Research
Volume: 40
Pages: e7
DOI
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[Journal Article] Structure-Based Design, Synthesis, and Evaluation of Peptide-mimetic SARS 3CL Protease Inhibitors2011
Author(s)
Akaji K, Konno H, Mitsui H, Teruya K, Shimamoto Y, Hattori Y, Ozaki T, Kusunoki M, Sanjoh A
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Journal Title
Journal of Medicinal Chemistry
Volume: 54
Pages: 7962-7973
DOI
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[Journal Article] Binding and Selectivity of the Marine Toxin Neodysiherbaine A, and its Synthetic Analogues, to GluK1 and GluK2 Kainate Receptors2011
Author(s)
Unno M, Shinohara M, Takayama K, Tanaka H, Teruya K, Doh-Ura K, Sakai R, Sasaki M, Ikeda-Saito M
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Journal Title
Journal of Molecular Biology
Volume: 413
Pages: 667-683
DOI
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[Presentation] Syntheses of Hydroxyethylamine Derivatives Containing Side-Chain Mimetic and Peptide Sequence for BACE1 inhibitor2012
Author(s)
Hattori, Y.; Akiyama, T.; Nohara, Y.; Nishizaki, A.; Deguchi, A.; Shimamoto, Y.; Teruya, K.; Konnno, H.; Akaji, K.
Organizer
AIMECS11
Place of Presentation
Tokyo, Japan
Year and Date
20121131-1202
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[Presentation] Evaluation of Hydroxymethylcarbonyl- and Hydroxyethylamine-type BACE1 Inhibiors Containing a Superior BACE1 Cleavage Sequence0
Author(s)
Kazuya Kobayashi, Tomomi Akiyama, Yukie Nohara, Saki Goto, Mami Okabe, Natsumi Sato, Miki Itoh, Kanami Ueno, Yasunao Hattori, Kenta Teruya, Akira Sanjho, Kenichi Akaji
Organizer
4th Asia-Pacific International Peptide Symposium (APIPS)
Place of Presentation
Osaka
Year and Date
00001108
