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2013 Fiscal Year Final Research Report

Analyses of ER-resident molecular chaperone-dependent nuclear membrane fusion during fertilization of Arabidopsis thaliana

Research Project

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Project/Area Number 23570051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNiigata University (2012-2013)
Nagoya University (2011)

Principal Investigator

NISHIKAWA Shuh-ichi  新潟大学, 自然科学系, 教授 (10252222)

Project Period (FY) 2011 – 2013
Keywords小胞体 / 分子シャペロン / 核膜融合 / 有性生殖 / シロイヌナズナ
Research Abstract

We showed that BiP, an Hsp70 molecular chaperone in the endoplasmic reticulum, function in the fusions of the outer and inner nuclear membranes of the polar nuclei with different sets of J-domain containing partner proteins during the female gametogenesis of A. thaliana. Live imaging analyses showed that the polar nuclei fusion defect can be recovered during the first endosperm nuclear division after fertilization and that the fusion of sperm nuclei at fertilization is required for proper endosperm proliferation. We also identified candidates of nuclear membrane proteins required for the polar nuclei fusion.

  • Research Products

    (14 results)

All 2014 2013 2012 2011

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results) Book (2 results)

  • [Journal Article] Multiple BiP genes of Arabidopsis thaliana are required for male gametogenesis and pollen competitiveness2014

    • Author(s)
      Maruyama, D., Sugiyama, T., Endo, T., and Nishikawa, S.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Pages: 801-810

    • Peer Reviewed
  • [Journal Article] The Plant Organelles Database 3 (PODB3) update 2014: integrating electron micrographs and new options for plant organelle research2014

    • Author(s)
      Mano, S., Nakamura, T., Kondo, M., Miwa, T., Nishikawa, S., Mimura, T., Nagatani, A., and Nishimura, M.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Pages: e1

    • Peer Reviewed
  • [Journal Article] Analyses of protein-protein interactions by in vivo photocrosslinking in budding yeast2013

    • Author(s)
      Shiota,T., Nishikawa, S., and Endo, T.
    • Journal Title

      Meth, Mol. Biol.

      Volume: 1033 Pages: 207-217

    • Peer Reviewed
  • [Journal Article] Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria2013

    • Author(s)
      Tamura, Y., Harada, Y., Nishikawa, S., Yamano, K., Kamiya, M., Shiota, T., Kuroda, T., Kuge, O., Sesaki, H., Imai, K., Tomii, K., and Endo, T.
    • Journal Title

      Cell Metab.

      Volume: 17 Pages: 709-718

    • Peer Reviewed
  • [Journal Article] Roles of Dom34:Hbs1 in non-stop protein clearance from translocators for normal organelle protein influx2012

    • Author(s)
      Izawa, T., Tsuboi, T., Kuroha, K., Inada, T., Nishikawa, S., and Endo, T.
    • Journal Title

      Cell Rep.

      Volume: 2 Pages: 447-453

    • Peer Reviewed
  • [Journal Article] Yos9p and Hrd1p mediate ER retention of misfolded proteins for ER- associated degradation2012

    • Author(s)
      Izawa, T., Nagai, H., Endo, T. and Nishikawa, S.
    • Journal Title

      Mol. Biol. Cell

      Volume: 23 Pages: 1283-1293

    • Peer Reviewed
  • [Presentation] 高温ストレス下での花粉成熟過程における小胞体品質管理の役割2014

    • Author(s)
      杉山智之, 山本雅也, 遠藤斗志也, 西川周一
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学,富山県
    • Year and Date
      2014-03-18
  • [Presentation] ER quality control plays key roles in sexual reproduction at an elevated temperature in Arabidopsis thaliana2013

    • Author(s)
      Tomoyuki Sugiyamaa, Masaya Yamamotob, Toshiya Endo and Shuh-ichi Nishikawa
    • Organizer
      The 3rd International Congress on Natural Sciences
    • Place of Presentation
      新潟大学五十嵐キャンパス,新潟県
    • Year and Date
      2013-10-13
  • [Presentation] 小胞体における異常タンパク質の処理機構:分子機構と高次機能2013

    • Author(s)
      西川周一
    • Organizer
      新潟生化学懇話会
    • Place of Presentation
      新潟大学脳研究所,新潟県
    • Year and Date
      2013-07-13
  • [Presentation] 雌性配偶体形成時の極核の核外膜融合は中央細胞における精核の融合と胚乳形成に必要である2013

    • Author(s)
      丸山大輔, 山本雅也, 東山哲也, 遠藤斗志也, 西川周一
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学,岡山県
    • Year and Date
      2013-03-22
  • [Presentation] 植物の環境ストレス応答における小胞体品質管理の役割2012

    • Author(s)
      西川周一
    • Organizer
      第12回日本蛋白質科学会年会ワークショップ「植物の環境感覚をささえる多様な蛋白質機能」
    • Place of Presentation
      名古屋国際会議場,愛知県
    • Year and Date
      2012-06-21
  • [Presentation] Regulation of plant growth and development by the Hsp70 system in the ER.2011

    • Author(s)
      Shuh-ichi Nishikawa
    • Organizer
      「奈良先端未来開拓コロキウム」シンポジウムProtein and Organelle Dynamics under Cellular Stress Conditions
    • Place of Presentation
      奈良先端科学技術大学院大学バイオサイエンス研究科,奈良県
    • Year and Date
      20110530-31
  • [Book] Using image-based resources : Databases for plant organelle dynamics and applications based on image information. Image and Video Processing2013

    • Author(s)
      Mano, S., Kimori, S., Takeda, T., Miwa, T., Nishikawa, S., Mimura, T., Nagatani, A., and Nishimura, M.
    • Publisher
      iConcept Press(ISBN : 978-1-477554-80-7)
  • [Book] 動的なタンパク質相互作用の「スナップショット」を撮る〜in vivoにおける部位特異的光架橋法2012

    • Author(s)
      塩田拓也, 西川周一, 遠藤斗志也
    • Total Pages
      30:1799-1805
    • Publisher
      実験医学

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Published: 2015-07-16  

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