2013 Fiscal Year Final Research Report
Analyses of ER-resident molecular chaperone-dependent nuclear membrane fusion during fertilization of Arabidopsis thaliana
Project/Area Number |
23570051
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
|
Research Institution | Niigata University (2012-2013) Nagoya University (2011) |
Principal Investigator |
|
Project Period (FY) |
2011 – 2013
|
Keywords | 小胞体 / 分子シャペロン / 核膜融合 / 有性生殖 / シロイヌナズナ |
Research Abstract |
We showed that BiP, an Hsp70 molecular chaperone in the endoplasmic reticulum, function in the fusions of the outer and inner nuclear membranes of the polar nuclei with different sets of J-domain containing partner proteins during the female gametogenesis of A. thaliana. Live imaging analyses showed that the polar nuclei fusion defect can be recovered during the first endosperm nuclear division after fertilization and that the fusion of sperm nuclei at fertilization is required for proper endosperm proliferation. We also identified candidates of nuclear membrane proteins required for the polar nuclei fusion.
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[Journal Article] The Plant Organelles Database 3 (PODB3) update 2014: integrating electron micrographs and new options for plant organelle research2014
Author(s)
Mano, S., Nakamura, T., Kondo, M., Miwa, T., Nishikawa, S., Mimura, T., Nagatani, A., and Nishimura, M.
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Journal Title
Plant Cell Physiol.
Volume: 55
Pages: e1
Peer Reviewed
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[Journal Article] Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria2013
Author(s)
Tamura, Y., Harada, Y., Nishikawa, S., Yamano, K., Kamiya, M., Shiota, T., Kuroda, T., Kuge, O., Sesaki, H., Imai, K., Tomii, K., and Endo, T.
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Journal Title
Cell Metab.
Volume: 17
Pages: 709-718
Peer Reviewed
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[Journal Article] Roles of Dom34:Hbs1 in non-stop protein clearance from translocators for normal organelle protein influx2012
Author(s)
Izawa, T., Tsuboi, T., Kuroha, K., Inada, T., Nishikawa, S., and Endo, T.
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Journal Title
Cell Rep.
Volume: 2
Pages: 447-453
Peer Reviewed
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[Book] Using image-based resources : Databases for plant organelle dynamics and applications based on image information. Image and Video Processing2013
Author(s)
Mano, S., Kimori, S., Takeda, T., Miwa, T., Nishikawa, S., Mimura, T., Nagatani, A., and Nishimura, M.
Publisher
iConcept Press(ISBN : 978-1-477554-80-7)
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