2013 Fiscal Year Final Research Report
Identification of novel IAA transport inhibitors and their functions
| Project/Area Number |
23570060
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Plant molecular biology/Plant physiology
|
|---|
| Research Institution | Tokyo Metropolitan University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Takeshi 農業生物資源研, 遺伝子組換え研究センター, 研究員 (50599983)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 環境 / 酵素 / 植物 / 生化学 / 発生・分化 / ケミカルゲノミクス |
|---|
| Research Abstract |
We established two chemical screening methods by assessing the effect on gravitropic curvature and on IAA quantity transported from tip sections using the maize coleoptile systems. We used HitFinder 10,000 chemical library and identified 8 candidate compounds of IAA efflux inhibitor and it was revealed that 4 compounds among them showed lethality and affected cellular trafficking of PIN protein. We also found 10 compounds which affected slightly IAA transport in maize coleoptiles even at higher concentrations. One of the compound was similar to that of synthetic auxin 2,4-D, and it is possible that it could preferentially inhibit IAA influx. These active compounds may represent new tools for dissecting mechanisms of IAA transport and for identifying new protein components involving in IAA transport systems.
|
-
-
[Journal Article] Blue-light regulation of ZmPHOT1 and ZmPHOT2 gene expression and the possible involvement of Zmphot1 in phototropism in maize coleoptiles2014
Author(s)
Suzuki, H., Okamoto, A., Kojima, A., Nishimura, T., Takano, M., Kagawa, T., Kadota, A., Kanegae, T., Koshiba, T.
-
Journal Title
Planta
Volume: (in press)
DOI
-
[Journal Article] YUCASIN is a potent inhibitor of YUCCA(s), a key enzyme in auxin biosynthesis2014
Author(s)
Nishimura, T., Hayashi, K., Suzuki, H., Gyohda, A., Takaoka, C., Sakaguchi, Y., Matsumoto, S., Kasahara, H., Sakai, T., Kato, J., Kamiya, Y., Koshiba, T.
-
Journal Title
Plant J.
Volume: 77
Pages: 352-366
DOI
-
[Journal Article] Identification of indole-3-acetic acid transport inhibitors including compounds affecting cellular PIN trafficking by two chemical screening approaches using maize coleoptile systems2012
Author(s)
Nishimura, T., Matano, N., Morishima, T., Kakinuma, C., Hayashi, K., Komano, T., Kubo, M., Hasebe, M., Kasahara, H., Kamiya, Y., Koshiba, T.
-
Journal Title
Plant Cell Physiol.(Editor-in-Chief's choice)
Volume: 53
Pages: 1671-1682
DOI
-
-
-
-
[Presentation] YUCASIN is a novel potent inhibitor of a key enzyme YUCCA(s) in plant auxin biosynthesis2013
Author(s)
Nishimura, T., Hayashi, K., Suzuki, H., Gyohda, A., Koshiba, T.
Organizer
Ann Meeting of Plant Signal Behav 2013
Place of Presentation
Vancouver, Canada
Year and Date
20130000
-
-
[Presentation] Changes in IAA distribution and expression pattern of ZmSAUR2 after photo-stimulation in maize coleoptiles2012
Author(s)
Kojima, A., Suzuki, H., Matsuda, S., Nishimura, N., Kadota, A., Koshiba, T.
Organizer
Plant Biology Congress
Place of Presentation
Freiburg, Germany
Year and Date
20120000
-
[Presentation] Identification of indole-3-acetic acid transport inhibitors including compounds affecting cellular PIN trafficking by two chemical screening approaches using maize coleoptile systems2012
Author(s)
Nishimura, T., Matano, N., Morishima, T., Kakinuma, C., Hayashi, K., Komano, T., Kubo, M., Hasebe, M., Kasahara, H., Kamiya, Y., Koshiba, T.
Organizer
Plant Cell Physiol.
Year and Date
20120000
-
-
[Presentation] トウモロコシ幼葉鞘を用いたIAA合成および輸送阻害剤のケミカルスクリーニング2012
Author(s)
松本さちこ, 西村岳志, 林謙一郎, 俣野由行, 森島大智, 坂口祐介, 駒野照弥, 久保稔, 長谷部光泰, 笠原博幸, 神谷勇治, 小柴共一
Organizer
日本植物生理学会第53回大会
Place of Presentation
京都
Year and Date
20120000
-
-
-
-
