Elucidation of a gene duplication and the gain of function of the estrogen receptor during the evolution

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Hokkaido University
• Principal Investigator: KATSU Yoshinao 北海道大学, 理学(系)研究科(研究院), 准教授 (00332180)
• Project Period (FY): 2011 – 2013
• Keywords: エストロゲン受容体 / ステロイドホルモン / 分子進化 / 比較内分泌

Research Abstract

The estrogen receptor interacts with estrogen which is female sex hormone, and participates in mechanism of the homeostatic maintenance of the vertebrates. However, it is yet unknown that the molecular evolution of estrogen receptor gene and protein. This research theme was carried out for the appearance of the estrogen receptor gene with the organic evolution and the elucidation of the process of the gain of function. As a result, it became clear that the elasmobranchii has the estrogen receptor genes that encode a functional receptor like higher vertebrates. Our functional analysis suggest that the function of estrogen receptor as a hormone-dependent transcription factor might be gain through the evolutionary process. The above-mentioned result will become the important clue in elucidating the molecular evolution of the estrogen receptor.

Research Products

• [Journal Article] Tyler CR, Iguchi T, Cloning, expression and functional characterization of carp, Cyprinus carpio, estrogen receptors and their differential activations by estrogens (2013)
  • Author(s): Katsu Y, Lange A, Miyagawa S, Urushitani H, Tatarazako N, Kawashima Y
  • Journal Title: J. Appl. Toxicol. Volume: 33 Pages: 41-49
  • DOI: 10.1002/jat.1707
  • Peer Reviewed
• [Journal Article] Establishment of transactivation assay systems using fish, amphibian, reptilian and human thyroid hormone receptors (2013)
  • Author(s): Oka T, Mitsui-Watanabe N, Tatarazako N, Onishi Y, Katsu Y, Miyagawa S, Ogino Y, Yatsu R, Kohno S, Takase M, Kawashima Y, Ohta Y, Aoki Y, Guillette LJ, Iguchi T
  • Journal Title: J. Applied Toxicology Volume: 33 Pages: 991-1000
  • DOI: 10.1002/jat.2825
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of the corticoid receptors from the American alligator (2013)
  • Author(s): Oka K, Kohno S, Urushitani H, Guillette LJ, Ohta Y, Iguchi T, Katsu Y
  • Journal Title: Mol. Cell. Endocrinol. Volume: 365 Pages: 153-161
  • DOI: 10.1016/j.mce.2012.10.014
  • Peer Reviewed
• [Journal Article] Cloning and characterization of the retinoic acid receptor-1 like protein in the rock shell, Thais clavigera (2013)
  • Author(s): Urushitani H, Katsu Y, Ohta Y, Shiraishi H, Iguchi T, Horiguchi T
  • Journal Title: Aquat. Toxicol. Volume: 142-143C Pages: 403-413
  • DOI: 10.1016/j.aquatox.2013.09.008
  • Peer Reviewed
• [Journal Article] Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring (2012)
  • Author(s): Lange A, Katsu Y, Miyagawa S, Ogino Y, Urushitani H, Kobayashi T, Hirai T, Shears JA, Nagae M, Yamamoto J, Ohnishi Y, Oka T, Tatarazako N, Ohta Y, Tyler CR, Iguchi T, ( These authors contributed equally)
  • Journal Title: Aquat. Toxicol. Volume: 109 Pages: 250-258
  • DOI: 10.1016/j.aquatox.2011.09.004
  • Peer Reviewed
• [Journal Article] Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17a-ethinylestradiol (2012)
  • Author(s): Hirakawa I, Miyagawa S, Katsu Y, Kagami Y, Tatarazako N, Kobayashi T, Kusano T, Mizutani T, Ogino Y, Takeuchi T, Ohta Y, Iguchi T
  • Journal Title: Chemosphere Volume: 87 Pages: 668-674
  • DOI: 10.1016/j.chemosphere.2011.12.047
  • Peer Reviewed
• [Journal Article] Wnt family genes and their modulation in the ovary-independent and persistent vaginal epithelial cell proliferation and keratinization induced by neonatal diethylstilbestrol exposure in mice (2012)
  • Author(s): Nakamura T, Miyagawa S, Katsu Y, Watanabe H, Mizutani T, Sato T, Morohashi KI, Takeuchi T, Iguchi T, Ohta Y
  • Journal Title: Toxicology Volume: 296 Pages: 13-19
  • DOI: 10.1016/j.tox.2012.02.010
  • Peer Reviewed
• [Journal Article] Estrogen receptors in medaka (Oryzias latipes) and estrogenic environmental contaminants : an in vitro-in vivo correlation (2011)
  • Author(s): Chakraborty T, Katsu Y, Zhou LY, Miyagawa S, Nagahama Y, Iguchi T
  • Journal Title: J. Steroid Biochem. Mol. Biol. Volume: 123 Pages: 115-121
  • DOI: 10.1016/j.jsbmb.2010.11.015
  • Peer Reviewed
• [Journal Article] Differential expression of three estrogen receptor subtype mRNAs in gonads and liver from embryos to adults of the medaka; Oryzias latipes (2011)
  • Author(s): Chakraborty T, Shibata Y, Zhou LY, Katsu Y, Iguchi T, Nagahama Y
  • Journal Title: Mol. Cell. Endocrinol. Volume: 333 Pages: 47-54
  • DOI: 10.1016/j.mce.2010.12.002
  • Peer Reviewed
• [Journal Article] Molecular cloning of anti-Müllerian hormone from the American alligator, Alligator mississippiensis (2011)
  • Author(s): Urushitani H, Katsu Y, Miyagawa S, Kohno S, Ohta Y, Guillette LJ, Iguchi T
  • Journal Title: Mol. Cell. Endocrinol. Volume: 333 Pages: 190-199
  • DOI: 10.1016/j.mce.2010.12.025
• [Journal Article] Altered gonadal expression of TGF-b superfamily signaling factors in environmental contaminant-exposed juvenile alligators (2011)
  • Author(s): Moore BC, Milnes MR, Kohno S, Katsu Y, Iguchi T, Woodruff TK, Guillette LJ
  • Journal Title: J. Steroid Biochem. Mol. Biol. Volume: 127 Pages: 58-63
  • DOI: 10.1016/j.jsbmb.2011.01.004
  • Peer Reviewed
• [Journal Article] Cloning and characterization of retinoid X receptor (RXR) isoforms in the rock shell Thais clavigera (2011)
  • Author(s): Urushitani H, Katsu Y, Ohta Y, Shiraishi H, Iguchi T, Horiguchi T
  • Journal Title: Aquat. Toxicol. Volume: 103 Pages: 101-111
  • DOI: 10.1016/j.aquatox.2011.02.012
  • Peer Reviewed
• [Journal Article] Metabolomics reveals target and off-target toxicities of a model organophosphate pesticide to roach (Rutilus rutilus): Implications for biomonitoring (2011)
  • Author(s): Southam, AD, Lange A, Hines A, Hill EM, Katsu Y, Iguchi T, Tyler CR, Viant MR
  • Journal Title: Environ. Sci. Technol. Volume: 45 Pages: 3759-3767
  • DOI: 10.1021/es103814d
  • Peer Reviewed
• [Presentation] 爬虫類の温度依存的な性決定のメカニズムの謎 (2013)
  • Author(s): 勝義直
  • Organizer: 日本比較内分泌学会
  • Place of Presentation: 宮崎
  • Year and Date: 2013-10-26
  • Invited
• [Presentation] ワニのアンドロゲン受容体の機能解析 (2013)
  • Author(s): 宮川信一, 河野郷通, 谷津遼平, 勝義直, Louis Guillette, 井口泰泉
  • Organizer: 日本比較内分泌学会
  • Place of Presentation: 宮崎
  • Year and Date: 2013-10-25
• [Presentation] 古代魚のコルチコイド受容体の単離 (2013)
  • Author(s): 勝義直, 岡香織, 杉本章, 井口泰泉
  • Organizer: 日本動物学会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-26
• [Presentation] 無顎類ヤツメウナギのエストロゲン受容体の単離 (2012)
  • Author(s): 勝義直, 井口泰泉
  • Organizer: 日本動物学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] アメリカアリゲーターのアリル炭化水素受容体の単離と機能解析 (2012)
  • Author(s): 岡香織, 太田康彦, 井口泰泉, 勝義直
  • Organizer: 日本動物学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] エストロゲン受容体の分子進化と機能進化 (2012)
  • Author(s): 勝義直
  • Organizer: 日本進化学会
  • Place of Presentation: 東京
  • Year and Date: 2012-08-21
  • Invited
• [Presentation] アメリカアリゲーターのコルチコイド受容体の単離と機能解析 (2011)
  • Author(s): 岡香織, 太田康彦, 井口泰泉, 勝義直
  • Organizer: 日本動物学会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] ゾウギンザメのエストロゲン受容体および機能解析 (2011)
  • Author(s): 成田晴香, 兵藤晋, 勝義直
  • Organizer: 日本動物学会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] 爬虫類のエストロゲン受容体の単離と種・リガンド特異性の解析 (2011)
  • Author(s): 勝義直, 松原和純, 松田洋一, 鳥羽通久, 岡香織, 太田康彦, 井口泰泉
  • Organizer: 日本動物学会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Remarks]
  • URL: http://www.repdev-katsu.jp/