Isolation and characterization of ubiquitin-like protein MNSF-conjugating enzymes

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Shimane University
• Principal Investigator: NAKAMURA Morihiko 島根大学, 産学連携センター, 教授 (20155865)
• Project Period (FY): 2011 – 2013
• Keywords: ユビキチン様タンパク質 / 結合酵素 / 翻訳後修飾

Research Abstract

In this study, we identified and characterized an enzyme which conjugates MNSF to target protein(s). This enzyme is a novel protein similar to ubiquitin conjugating enzyme E3. In addition, we also identified a heat shock protein which stabilizes aggregative MNSF. Thus, the mechanism of MNSF conjugation was partly shown.

Research Products

• [Journal Article] Ubiquitin-like protein MNSFβnegatively regulates T cell function and survival (2014)
  • Author(s): Nakamura M, Nakagawa M, Watanabe J.
  • Journal Title: Immunol Invest Volume: (in press)
  • Peer Reviewed
• [Journal Article] Ubiquitin-like protein MNSFβcovalently binds to Bcl-G and enhances lipopolysaccharide (LPS)/interferonγ(IFNγ)-induced apoptosis in macrophages (2013)
  • Author(s): Wtanabe J, Nakagawa M, Nakamura M.
  • Journal Title: FEBS J. Volume: 280 Pages: 1281-93
  • Peer Reviewed
• [Journal Article] Preliminary study on using accelerometers to measure involuntary movement for the assessment of neurological motor impairments (2013)
  • Author(s): Iwasaki Y, Hirotomi T, Oguro H, Nakamura M.
  • Journal Title: Advanced Applied Informatics Volume: 46 Pages: 33-38
  • Peer Reviewed
• [Journal Article] Ubiquitin-like protein MNSFβregulates TLR-2-mediated signal transduction (2012)
  • Author(s): Nakamura M, Watanabe J, Watanabe N.
  • Journal Title: Mol Cell Biochem Volume: 364 Pages: 39-43
  • Peer Reviewed
• [Journal Article] New surface-modified zinc oxide nanoparticles with aminotriethylene oxide chains linked by 1,2,3-triazole ring : preparation, and visible light-emitting and noncytotoxic properties (2012)
  • Author(s): Sato M, Shimatani K, Iwasaki Y, Morito S., Tanaka H, Fujita Y, Nakamura M.
  • Journal Title: Applied Surface Science Volume: 258 Pages: 786-790
  • Peer Reviewed
• [Journal Article] Chronic administration of cardanol (Ginkgol) extracted from Ginkgo biloba leaves and cashew nutshell liquid improves Working Memory-Related learning in rats (2012)
  • Author(s): Tobinaga S, Hashimoto M, Utsunomiya I, Taguchi K, Nakamura M, Tsunematsu T.
  • Journal Title: Biol Pharm Bull Volume: 35 Pages: 127-129
  • Peer Reviewed
• [Presentation] Isolation and characterization of ubiquitin-like protein MNSFβconjugating enzymes (2013)
  • Author(s): Nakagawa M, Nakamura M
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-11
• [Presentation] Preliminary study on using accelerometers to measure involuntary movements for the assessment of neurological motor impairments (2013)
  • Author(s): Iwasaki Y, Hirotomi T, Oguro H, Nakamura M.
  • Organizer: Second IIAI International Conference on Advanced Applied Informatics Matsue
  • Place of Presentation: Japan
  • Year and Date: 2013-09-04
• [Presentation] Ubiquitin- like protein MNSFβcovalently binds to Bcl-G and enhances lipopolysaccharide (LPS)/interferonγ(IFNγ)-induced apoptosis in macrophages (2013)
  • Author(s): Nakamura M, Nakagawa M.
  • Organizer: 15^<th> International Congress of Immunology Milan
  • Place of Presentation: Italy
  • Year and Date: 2013-08-24
• [Presentation] Usefulness of aromatherapy ("Shimane rose water") on bathing process for elderly with dementia living in a geriatric health service facility (2013)
  • Author(s): Takeda Y, Hara S, Hasegawa S, Ono M, Nakamura M.
  • Organizer: Alzheimer's Disease International (ADI)
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2013-04-20
• [Presentation] Ubiquitin-like protein MNSFβregulates apoptosis through ERK/ AP-1 pathway in LPS/IFNγ- stimulated macrophages (2012)
  • Author(s): Watanabe J, Nakagawa M, Nakamura M.
  • Organizer: 第85回日本生化学会
  • Place of Presentation: 博多
  • Year and Date: 2012-12-14
• [Presentation] Ubiquitin-like protein MNSFβenhances LPS/IFNγ- induced apoptosis in macrophages (2011)
  • Author(s): Watanabe J, Watanabe N, Nakamura M.
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Remarks]
  • URL: http://www.ed.shiane-u.ac.jp/CMRC/index2.html
• [Patent(Industrial Property Rights)] 水溶性超常磁性ナノ粒子 (2013)
  • Inventor(s): 佐藤守之, 中村守彦
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-002041号
  • Filing Date: 2013-01-09
  • Overseas
• [Patent(Industrial Property Rights)] 蛍光標識材料および蛍光標識剤 (2013)
  • Inventor(s): 佐藤守之, 中村守彦
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 5326078号
  • Acquisition Date: 2013-08-02
  • Overseas
• [Patent(Industrial Property Rights)] Fluorescent labeling material and fluorescent labeling agent (2013)
  • Inventor(s): Sato M, Nakamura M.
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 09823469.3
  • Acquisition Date: 2013-09-25
  • Overseas
• [Patent(Industrial Property Rights)] 情報ネットワークシステム (2012)
  • Inventor(s): 橋口尚幸, 中村守彦, 四郎丸功, 増成博志
  • Industrial Property Rights Holder: 島根大学長
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-013088号
  • Filing Date: 2012-01-25
  • Overseas