2013 Fiscal Year Final Research Report
Investigation into the LINE transposition process by structural study of DNA-protein complexes
| Project/Area Number |
23570139
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MAITA Nobuo 徳島大学, 疾患酵素学研究センター, 准教授 (00404046)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Ｘ線結晶構造解析 / レトロトランスポゾン |
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| Research Abstract |
The aim of this project is to reveal the structural basis of the LINE. To obtain the stable protein, I tested four EN-Myb constructs, but none could be expressed or purified better than Sart1-ORF2p. Next, I used Sf9 cells as protein expression system to overcome the codon-usage issue. Although I detected the ORF1p expression, ORF2p was not co-expressed. I tried to purify the ORF1p expressed in Sf9 cells, however, ORF1p was recovered in insoluble fraction and failed to purify.
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Research Products
(13 results)
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[Journal Article] Structural basis for simultaneous recognition of an O-glycan and its attached peptide of mucin family by immune receptor PILRα2014
Author(s)
Kuroki K, Wang J, Ose T, Yamaguchi M, Tabata S, Maita N, Nakamura S, Kajikawa M, Kogure A, Satoh T, Arase H, Maenaka K.
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Journal Title
Proc Natl Acad Sci USA
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] Observation of the controlled assembly of preclick components in the in situ click chemistry generation of a chitinase inhibitor2013
Author(s)
Hirose T, Maita N, Gouda H, Koseki J, Yamamoto T, Sugawara A, Nakano H, Hirono S, Shiomi K, Watanabe T, Taniguchi H, Sharpless KB,Ōmura S, Sunazuka T.
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Journal Title
Proc Natl Acad Sci USA
Volume: 110
Pages: 15892-15897
DOI
Peer Reviewed
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