Towards the molecular basis of the ligand-binding mechanism and drug-discovery of TAM receptor tyrosine kinases

2014 Fiscal Year Final Research Report

• Project/Area Number: 23570146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: NAGATA Takashi 京都大学, エネルギー理工学研究所, 准教授 (10415250)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Hideo 横浜市立大学, その他の研究科, 教授 (60265717)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: TAM受容体 / NMR / 糖鎖修飾

Outline of Final Research Achievements

TAM receptor tyrosine kinases have causal relationships with diseases. We have set up the methods to obtain Tyro3 and Mer of the family using yeast K. lactis, which can potentially glycosylate the proteins; to introduce stable isotope into proteins in both uniform and selective manner; to analyze glycosylation modifications; to analyze molecular interactions; and to run and analyze several NMR methods. We have then applied these methods to Tyro3 and Mer, and evaluated the influences of glycosylation on their structures and functions. We have also obtained the methods to develop peptides and nucleic acids that bind to the target proteins, and the methods to evaluate the structure and function of the obtained molecules. On the basis of current efforts, we are going to collect structural information of TAM receptors in action, and are intending to bring the project further to elucidate the molecular mechanisms underlying the recognition of ligands or drug candidates by TAM receptors.

Free Research Field

構造生命科学