2013 Fiscal Year Final Research Report
Analysis of erythroid differentiation by HSP27
| Project/Area Number |
23570172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Keio University |
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Principal Investigator |
KABE Yasuaki 慶應義塾大学, 医学部, 講師 (20397037)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIMORI Koichiro 北海道大学, 理学部, 教授 (20192487)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ヘム / 赤血球分化 |
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| Research Abstract |
During erythropoiesis, haem synthesis is significantly induced, and abundant haem is utilized as hemoglobin in erythroid cells. However, excessive haem results in cell damages by membrane oxidation. In the present study, we found that haem directly bound to heat shock protein 27 (HSP27) by affinity purification. HSP27 is a small heat shock family protein, which plays an important role for cytoprotection, stress tolerance, cellular differentiation or tissue development. It has been known that the multimerized HSP27 dissociates by several stimuli. Interestingly, exposure of haem directly dissociated the multimerized HSP27 in vivo and in vitro. Knockdown of HSP27 expression resulted in the haem-induced apoptosis and reduced the haemin-induced erythroid differentiation. Furthermore, we also found that HSP27 is essential for erythroid differentiation by using HSP27 knockdown mice.
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Research Products
(19 results)
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[Journal Article] Microscopic imaging mass spectrometry assisted by on-tissue chemical derivatization for visualizing multiple amino acids in human colon cancer xenografts2013
Author(s)
Toue S, Sugiura Y, Kubo A, Ohmura M, Karakawa S, Mizukoshi T, Yoneda J, Miyano H, Noguchi Y, Kobayshi T, Kabe Y, Suematsu M.
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Journal Title
Proteomics
Volume: 14(7-8)
Pages: 810-9
Peer Reviewed
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[Journal Article] Vesnarinone suppresses TNFαmRNA expression by inhibiting valosin-containing protein2013
Author(s)
Hotta K, Nashimoto A, Yasumura E, Suzuki M, Azuma M, Iizumi Y, Shima D, Nabeshima R, Hiramoto M, Okada A, Sakata-Sogawa K, Tokunaga M, Ito T, Ando H, Sakamoto S, Kabe Y, Aizawa S, Imai T, Yamaguchi Y, Watanabe H, Handa H.
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Journal Title
Mol Pharmacol
Volume: 83(5)
Pages: 930-8
Peer Reviewed
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[Journal Article] Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway2012
Author(s)
Morikawa T, Kajimura M, Nakamura T, Hishiki T, Nakanishi T, Yukutake Y, Nagahata Y, Ishikawa M, Hattori K, Takenouchi T, Takahashi T, Ishii I, Matsubara K, Kabe Y, Uchiyama S, Nagata E, Gadalla MM, Snyder SH, Suematsu M.
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Journal Title
Proc Natl Acad Sci U S A
Volume: 109(4)
Pages: 1293-8
Peer Reviewed
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[Journal Article] HIF-1αinduction suppresses excessive lipid accumulation in alcoholic fatty liver in mice2012
Author(s)
Nishiyama Y, Goda N, Kanai M, Niwa D, Osanai K, Yamamoto Y, Senoo-Matsuda N, Johnson RS, Miura S, Kabe Y, Suematsu M.
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Journal Title
J Hepatol.
Volume: 56(2)
Pages: 441-7
Peer Reviewed
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[Journal Article] Identification of dynamin-2-mediated endocytosis as a new target of osteoporosis drugs, bisphosphonates2011
Author(s)
Masaike Y, Takagi T, Hirota M, Yamada J, Ishihara S, Yung TM, Inoue T, Sawa C, Sagara H, Sakamoto S, Kabe Y, Takahashi Y, Yamaguchi Y, Handa H.
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Journal Title
Mol Pharmacol.
Volume: 77(2)
Pages: 262-9
Peer Reviewed
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[Book] 実験医学「がんと代謝」2012
Author(s)
末松誠, 久保亜紀子, 大村光代, 菱木貴子, 梶村真弓, 加部泰明, 杉浦悠基
Total Pages
193-202 (著書・総説)
Publisher
羊土社
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