2013 Fiscal Year Final Research Report
Protein assembly at DNA replication fork and role of replication DNA helicase
| Project/Area Number |
23570204
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Ibaraki University |
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Principal Investigator |
ISHIMI Yukio 茨城大学, 理学部, 教授 (80159772)
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| Project Period (FY) |
2011 – 2013
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| Keywords | MCMヘリカーゼ / DNA複製フォーク / タンパク質相互作用 |
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| Research Abstract |
DNA replication fork progressed by MCM2-7 helicase is regulated by action of MCM interacting proteins. I clarified interaction of MCM2-7 with the MCM interacting proteins. CDC45 that is a component of CMG complex extensively interacted with all of MCM2-7. Similarly MCM-BP that is probably involved in inhibition of MCM2-7 complex interacted all of MCM2-7. This feature may be important for regulation of MCM function through structural change of MCM2-7 complex. Other MCM interactants bound to ATP binding domain of MCM6 and this binding may play a role in regulation of MCM function, since ATP binding to
MCM6 is critical for MCM function.
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Research Products
(13 results)
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[Journal Article] Translocation and stability of replicative DNA helicases upon encountering DNA-protein cross-links2013
Author(s)
Nakano, T., Miyamoto-Matsubara, M., Shoulkamy, M.I., Salem, A.M., Pack, S.P., Ishimi, Y. and Ide, H.
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Journal Title
J. Biol. Chem.
Volume: 288
Pages: 4649-4658
Peer Reviewed
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