2013 Fiscal Year Final Research Report
Chromatin regulation by DNA replication and recombination
| Project/Area Number |
23570212
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
NAKAGAWA TAKURO 大阪大学, 理学(系)研究科(研究院), 准教授 (20324866)
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| Research Collaborator |
MASUKATA Hisao 大阪大学, 大学院理学研究科, 教授 (00199689)
TAKAHASHI Tatsuro 大阪大学, 大学院理学研究科, 助教 (50452420)
ZAFAR Faria 大阪大学, 大学院理学研究科, 博士課程
OKITA Akiko 大阪大学, 大学院理学研究科, 博士課程
ONAKA Atsushi 大阪大学, 大学院理学研究科, 博士課程
SAGAWA Minami 大阪大学, 大学院理学研究科, 修士課程
SU Jie 大阪大学, 大学院理学研究科, 修士課程
TOYOFUKU Naoko 大阪大学, 大学院理学研究科, 修士課程
KATAHIRA Yasuhiro 大阪大学, 大学院理学研究科, 修士課程
INOUE Takahiro 大阪大学, 大学院理学研究科, 修士課程
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| Project Period (FY) |
2011 – 2013
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| Keywords | DNA複製 / DNA組換え / セントロメア / クロマチン / 分裂酵母 |
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| Research Abstract |
Chromatin structures affect the pathway choice of DNA recombination. We found that the chromatin structures formed in centromere prevents single-strand annealing (SSA) and crossing over to take place. This centromere-specific regulation of recombination is important to prevent gross chromosomal rearrangement (GCR) in centromere.
Numbers of pre-replicative complexes (preRCs) are assembled on each chromosome in eukaryotes. We found that the formation of preRCs is important not only for DNA replication per se but also for the repair of DNA double-strand breaks (DSBs) and for the construction of chromatin structures in centromere probably through histone acetylation.
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[Journal Article] The DNA damage checkpoint pathway promotes extensive resection and nucleotide synthesis to facilitate homologous recombination repair and genome stability in fission yeast2014
Author(s)
Blaikley, E. J., Tinline-Purvis, H., Kasparek, T. R., Marguerat, S., Sarkar, S., Hulme, L., Hussey, S., Wee, B. Y., Deegan, R. S., Walker, C. A., Pai, C. C., Bahler, J., Nakagawa, T., and Humphrey, T. C.
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Journal Title
Nucleic Acids Res
Volume: 42
Pages: 5644-5656
DOI
Peer Reviewed
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[Journal Article] Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast2012
Author(s)
Tazumi, A., Fukuura, M., Nakato, R., Kishimoto, A., Takenaka, T., Ogawa, S., Song, J. H., Takahashi, T. S., Nakagawa, T., Shirahige, K., and Masukata, H.
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Journal Title
Genes Dev
Volume: 26
Pages: 2050-2062
DOI
Peer Reviewed
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[Journal Article] The prereplication complex recruits XEco2 to chromatin to promote cohesin acetylation in Xenopus egg extracts2012
Author(s)
Higashi, T. L., Ikeda, M., Tanaka, H., Nakagawa, T., Bando, M., Shirahige, K., Kubota, Y., Takisawa, H., Masukata, H., and Takahashi, T. S.
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Journal Title
Curr Biol
Volume: 22
Pages: 977-988
DOI
Peer Reviewed
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[Journal Article] Abundance of prereplicative complexes (Pre-RCs) facilitates recombinational repair under replication stress in fission yeast2011
Author(s)
Maki, K., Inoue, T., Onaka, A., Hashizume, H., Somete, N., Kobayashi, Y., Murakami, S., Shigaki, C., Takahashi, T. S., Masukata, H., and Nakagawa, T.
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 41701-41710
DOI
Peer Reviewed
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[Journal Article] Auxin-inducible protein depletion system in fission yeast2011
Author(s)
Kanke, M., Nishimura, K., Kanemaki, M., Kakimoto, T., Takahashi, T. S., Nakagawa, T., and Masukata, H.
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Journal Title
BMC Cell Biol
Volume: 12
Pages: 8
DOI
Peer Reviewed
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[Journal Article] CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast2011
Author(s)
Fukuura, M., Nagao, K., Obuse, C., Takahashi, T. S., Nakagawa, T., and Masukata, H.
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Journal Title
Mol Biol Cell
Volume: 22
Pages: 2620-2633
DOI
Peer Reviewed
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